CD40 Protein, Canine (HEK293)

Cat. No.: HY-P75413: FAQs
Data Sheet Handling Instructions

CD40 Protein, the receptor for TNFSF5/CD40LG, transduces signals via TRAF6 and MAP3K8, activating ERK in macrophages and B cells, leading to immunoglobulin secretion. Existing as a monomer and homodimer, CD40 interacts with TRAF proteins (TRAF1, TRAF2, TRAF3, TRAF5, and TRAF6), with its interaction with TRAF6 and MAP3K8 crucial for ERK activation. CD40 Protein, Canine (HEK293) is the recombinant canine-derived CD40 protein, expressed by HEK293 , with tag free. The total length of CD40 Protein, Canine (HEK293) is 194 a.a., with molecular weight of 28-32 kDa.

For research use only. We do not sell to patients.

Protein Expression Service

Size		Stock
50 μg		Get quote
100 μg		Get quote
Other size		Get quote

* Please select Quantity before adding items.

Biological Activity
Technical Parameters
Properties
Documentation

Description	CD40 Protein, the receptor for TNFSF5/CD40LG, transduces signals via TRAF6 and MAP3K8, activating ERK in macrophages and B cells, leading to immunoglobulin secretion. Existing as a monomer and homodimer, CD40 interacts with TRAF proteins (TRAF1, TRAF2, TRAF3, TRAF5, and TRAF6), with its interaction with TRAF6 and MAP3K8 crucial for ERK activation. CD40 Protein, Canine (HEK293) is the recombinant canine-derived CD40 protein, expressed by HEK293 , with tag free. The total length of CD40 Protein, Canine (HEK293) is 194 a.a., with molecular weight of 28-32 kDa.
Background	CD40 Protein acts as the receptor for TNFSF5/CD40LG, transducing signals mediated by TRAF6 and MAP3K8 to activate ERK in macrophages and B cells, ultimately inducing immunoglobulin secretion. Existing as both a monomer and homodimer, CD40 Protein interacts with various TRAF proteins, including TRAF1, TRAF2, TRAF3, TRAF5, and TRAF6. Furthermore, its interaction with TRAF6 and MAP3K8 is essential for the activation of ERK.
Species	Canine
Source	HEK293
Tag	Tag Free
Accession	Q7YRL5 (M1-A194)
Gene ID	403469 [NCBI]
Molecular Construction	N-term CD40 (M1-A194) Accession # Q7YRL5 C-term
Synonyms	Tumor Necrosis Factor Receptor Superfamily member 5; Bp50; CD40L Receptor; CDw40; TNFRSF5
Molecular Weight	28-32 kDa
Purity	Greater than 95% as determined by reducing SDS-PAGE
Endotoxin Level	<1 EU/μg, determined by LAL method.
Documentation	Data Sheet (232 KB) Handling Instructions (2659 KB)

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Reconstitution Calculator
Dilution Calculator
Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial)	=	Mass (in vial)	÷	Desired Reconstitution Concentration
	=		÷

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)	×	Volume _(start)	=	Concentration _(final)	×	Volume _(final)
	×		=		×
C1		V1		C2		V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 10⁶ ÷ Biological Activity (ED₅₀)

Specific Activity (Unit/mg)	=	10⁶	÷	Biological Activity (ED₅₀)
Unit/mg	=	10⁶	÷	ng/mL

Your Recently Viewed Products:

Product Name

Salutation

Applicant Name *

Email Address *

Phone Number *

Organization Name *

Department *

Requested quantity *

Country or Region *

Remarks

Product Name			Lot #
Applicant Name *			Country or Region *
Email Address *
Phone Number *			Organization Name *
Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

CD40 Protein, Canine (HEK293)

Biological Activity

Technical Parameters

Properties

Documentation