Copy Link Print Information

CD52 Protein, Mouse (HEK293, Fc)

Customer Review

Based on 1 Customer Validation

CD52 protein carries and orients carbohydrates, suggesting involvement in molecular transport and arrangement.Its multifaceted role includes a specific function that is not fully understood.Further exploration into CD52's mechanisms and contributions can shed light on its broader significance.Understanding its targeted role provides valuable insights into its functions and implications.CD52 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived CD52 protein, expressed by HEK293 , with C-hFc labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for CD52 Protein, Mouse (HEK293, Fc)
  • Experimental Validation Results for CD52 Protein, Mouse (HEK293, Fc)
  • CD52 Protein, Mouse (HEK293, Fc) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

CD52 protein carries and orients carbohydrates, suggesting involvement in molecular transport and arrangement.Its multifaceted role includes a specific function that is not fully understood.Further exploration into CD52's mechanisms and contributions can shed light on its broader significance.Understanding its targeted role provides valuable insights into its functions and implications.CD52 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived CD52 protein, expressed by HEK293 , with C-hFc labeled tag.

Background

The CD52 protein appears to have a multifaceted role, involving both the carrying and orienting of carbohydrates, indicating potential functions in molecular transport and arrangement. Beyond these general activities, CD52 may also harbor a more specific role, the details of which are yet to be fully understood. The versatility suggested by CD52's dual functions prompts further exploration into its precise mechanisms and contributions, shedding light on its broader significance in cellular and molecular contexts. Unraveling the specifics of CD52's more targeted role could provide valuable insights, advancing our understanding of its intricate functions and potential implications.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Human Siglec-10 is immobilized at 2.5 µg/mL (100 µL/well) can bind Recombinant Mouse CD52 Protein. The ED50 for this effect is 1.71 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. When Recombinant Human Siglec-10 is immobilized at 2.5 µg/mL (100 µL/well) can bind Recombinant Mouse CD52 Protein. The ED50 for this effect is 1.71 μg/mL.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-hFc
  • Accession

    Q64389 (G24-K46)

  • Gene ID
    23833  [NCBI]
  • Molecular Construction
    • N-term
    • CD52 (M1-K46)
      Accession # Q64389
    • hFc
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    CD52; Cambridge Pathology 1 Antigen; Prev. CDW52; CDW52 Antigen (CAMPATH-1 Antigen); HE5; CD52 Antigen; CAMPATH-1 Antigen; CD52 Protein; EDDM5; HEL-S-171mP; Human Epididymis-Specific Protein 5; CDw52; CD52 Antigen (CAMPATH-1 Antigen); He5; Epididymal Secr

  • AA Sequence

    GQATTAASGTNKNSTSTKKTPLK

  • Molecular Weight

    Approximately 35-45 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. When Recombinant Human Siglec-10 is immobilized at 2.5 µg/mL (100 µL/well) can bind Recombinant Mouse CD52 Protein. The ED50 for this effect is 1.71 μg/mL.

  • CD52 Protein, Mouse (HEK293, Fc) Product Image
    1/16
  • Experimental Validation Results for CD52 Protein, Mouse (HEK293, Fc)
  • Experimental Validation Results for CD52 Protein, Mouse (HEK293, Fc)
  • CD52 Protein, Mouse (HEK293, Fc) Product Image