1. Recombinant Proteins
  2. CD73/5'-Nucleotidase Protein, Cynomolgus (HEK 293, His)

CD73/5'-Nucleotidase Protein, Cynomolgus (HEK 293, His)

Cat. No.: HY-P7836
Handling Instructions

CD73/5'-Nucleotidase Protein, Cynomolgus (HEK 293, His) is a CD73 protein with a His-flag. CD73/5'-Nucleotidase Protein a zinc-dependent, 70 kDa homodimeric enzyme that binds to the cell membrane through a glycosyl phophatidylinositol (GPI) anchor[1].

For research use only. We do not sell to patients.

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  • Technical Parameters

  • Properties

  • Documentation

Synonyms
rCynEcto-5'-nucleotidase/CD73, His; 5'-Nucleotidase; 5'-NT; Ecto-5'-Nucleotidase; CD73; NT5E; NT5; NTE
Species

Cynomolgus

Source

HEK 293

Accession

G7P3J1 (W27-K547)

Molecular Weight

58-80 kDa

AA Sequence

WELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFETDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHADEMFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEIYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRIK

Purity

Greater than 95% as determined by reducing SDS-PAGE

Biological Activity
2 μg/mL (100 μL/well) of immobilized recombinant Cynomolgus Ecto-5'-nucleotidase/CD73-His can bind Anti-Human CD73 mAb with an ED50 value of 9.68 ng/mL.
Appearance

Solution

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 120 mM NaCl, 4 mM CaCl2, 20% Glycerol, pH 7.5.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C. It is stable at -20℃ for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Background

CD73/5'-Nucleotidase is a zinc-dependent, 70 kDa homodimeric enzyme bound to the cell membrane through a glycosyl phophatidylinositol (GPI) anchor
. Vertebrate ecto-5'-nucleotidase (eN, EC 3.1.3.5) hydrolyzes ribo- and deoxyribonucleoside 5′-monophosphates including AMP, CMP, UMP, IMP, and GMP, whereby AMP generally is the most effectively hydrolyzed nucleotide
. CD73 is thought to function as a co-signaling molecule on T lymphocytes and an adhesion molecule that is required for lymphocyte binding to endothelium[1].[2]

Documentation
References
  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Product Name:
CD73/5'-Nucleotidase Protein, Cynomolgus (HEK 293, His)
Cat. No.:
HY-P7836
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