CD73/5'-Nucleotidase Protein, Cynomolgus (HEK293, His)
Based on 1 Customer Validation
CD73/5'-Nucleotidase Protein, Cynomolgus (HEK 293, His) is a CD73 protein with a His-flag. CD73/5'-Nucleotidase Protein a zinc-dependent, 70 kDa homodimeric enzyme that binds to the cell membrane through a glycosyl phophatidylinositol (GPI) anchor.
- Species: Cynomolgus
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
CD73/5'-Nucleotidase Protein, Cynomolgus (HEK 293, His) is a CD73 protein with a His-flag. CD73/5'-Nucleotidase Protein a zinc-dependent, 70 kDa homodimeric enzyme that binds to the cell membrane through a glycosyl phophatidylinositol (GPI) anchor[1].
Background
CD73/5'-Nucleotidase is a zinc-dependent, 70 kDa homodimeric enzyme bound to the cell membrane through a glycosyl phophatidylinositol (GPI) anchor. Vertebrate ecto-5'-nucleotidase (eN, EC 3.1.3.5) hydrolyzes ribo- and deoxyribonucleoside 5′-monophosphates including AMP, CMP, UMP, IMP, and GMP, whereby AMP generally is the most effectively hydrolyzed nucleotide. CD73 is thought to function as a co-signaling molecule on T lymphocytes and an adhesion molecule that is required for lymphocyte binding to endothelium[1].[2]
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Cynomolgus
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Source HEK293
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Tag C-6*His
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Accession
G7P3J1 (W27-K547)
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Gene ID/
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Molecular Construction
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N-term
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CD73 (W27-K547)
Accession # G7P3J1 -
6*His
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C-term
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Protein Length
Partial
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Synonyms
NT5E; ENT; Prev. NT5; 5'-NT; IMP-Specific 5'-Nucleotidase; NTE; Thymidylate 5'-Phosphatase; 5'-Nucleotidase, Ecto (CD73); 5'-Deoxynucleotidase; Purine 5-Prime-Nucleotidase; Ecto-5'-Nucleotidase; 5' Nucleotidase (CD73); 5'-Nucleotidase; CD73 Antigen; CALJA
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AA Sequence
WELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFETDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHADEMFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEIYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRIK
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Predicted Molecular Mass
58.8 kDa
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Molecular Weight
Approximately 58-80 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 120 mM NaCl, 4 mM CaCl2, 20% glycerol, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (265 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Herbert Zimmermann, ET AL. Cellular function and molecular structure of ecto-nucleotidases. Purinergic Signal. 2012 Sep;8(3):437-502. [Content Brief]
[2]. Dachuan Jin, et al. CD73 on tumor cells impairs antitumor T-cell responses: a novel mechanism of tumor-induced immune suppression. Cancer Res. 2010 Mar 15;70(6):2245-55. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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