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CDCP1 Protein, Human (HEK293, His)

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Based on 1 Customer Validation

CDCP1 protein may be involved in cell adhesion and extracellular matrix association. Phosphorylation of CDCP1 may regulate the balance between anchorage and migration, proliferation, and differentiation. CDCP1 Protein, Human (HEK293, His) is the recombinant human-derived CDCP1 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for CDCP1 Protein, Human (HEK293, His)
  • Experimental Validation Results for CDCP1 Protein, Human (HEK293, His)
  • CDCP1 Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

CDCP1 protein may be involved in cell adhesion and extracellular matrix association. Phosphorylation of CDCP1 may regulate the balance between anchorage and migration, proliferation, and differentiation. CDCP1 Protein, Human (HEK293, His) is the recombinant human-derived CDCP1 protein, expressed by HEK293 , with C-His labeled tag.

Background

CDCP1 protein is potentially involved in cell adhesion and the association with the extracellular matrix. Its phosphorylation may play a role in regulating the balance between anchorage and migration, as well as proliferation and differentiation. Additionally, CDCP1 has shown promise as a novel marker for leukemia diagnosis and for identifying immature hematopoietic stem cell subsets. It belongs to the tetraspanin web, which is implicated in tumor progression and metastasis. CDCP1 interacts with several proteins including CDH2/N-cadherin, CDH3/P-cadherin, SDC1/syndecan-1, SDC4/syndecan-4, the serine protease ST14/MT-SP1, as well as SRC and PRKCG/protein kinase C gamma, suggesting its involvement in various cellular processes and signaling pathways.

Verified Bioactivity

1.Immobilized Human CDCP1 at 2 μg/mL (100μL/Well). Dose response curve for Anti-CDCP1 Antibody with the EC50 < 8.0 ng/mL determined by ELISA.
2.Measured by its binding ability in a functional ELISA. When Recombinant Human CDCP1 is immobilized at 1 µg/mL (100 µL/well) can bind Human P-Cadherin . The ED50 for this effect is 1.046 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. When Recombinant Human CDCP1 is immobilized at 1 µg/mL (100 µL/well) can bind Human P-Cadherin. The ED50 for this effect is 1.046 μg/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    Q9H5V8-1 (F30-L666)

  • Gene ID
    64866  [NCBI]
  • Molecular Construction
    • N-term
    • CDCP1 (F30-L666)
      Accession # Q9H5V8-1
    • His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    CDCP1; Subtractive Immunization M Plus HEp3-Associated 135 KDa Protein; CUB Domain Containing Protein 1; Transmembrane And Associated With Src Kinases; SIMA135; Membrane Glycoprotein Gp140; CUB Domain-Containing Protein 1; TRASK; CD318; CD318 Antigen

  • AA Sequence

    FEIALPRESNITVLIKLGTPTLLAKPCYIVISKRHITMLSIKSGERIVFTFSCQSPENHFVIEIQKNIDCMSGPCPFGEVQLQPSTSLLPTLNRTFIWDVKAHKSIGLELQFSIPRLRQIGPGESCPDGVTHSISGRIDATVVRIGTFCSNGTVSRIKMQEGVKMALHLPWFHPRNVSGFSIANRSSIKRLCIIESVFEGEGSATLMSANYPEGFPEDELMTWQFVVPAHLRASVSFLNFNLSNCERKEERVEYYIPGSTTNPEVFKLEDKQPGNMAGNFNLSLQGCDQDAQSPGILRLQFQVLVQHPQNESNKIYVVDLSNERAMSLTIEPRPVKQSRKFVPGCFVCLESRTCSSNLTLTSGSKHKISFLCDDLTRLWMNVEKTISCTDHRYCQRKSYSLQVPSDILHLPVELHDFSWKLLVPKDRLSLVLVPAQKLQQHTHEKPCNTSFSYLVASAIPSQDLYFGSFCPGGSIKQIQVKQNISVTLRTFAPSFQQEASRQGLTVSFIPYFKEEGVFTVTPDTKSKVYLRTPNWDRGLPSLTSVSWNISVPRDQVACLTFFKERSGVVCQTGRAFMIIQEQRTRAEEIFSLDEDVLPKPSFHHHSFWVNISNCSPTSGKQLDLLFSVTLTPRTVDL

  • Molecular Weight

    Approximately 100-130 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5-8% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. When Recombinant Human CDCP1 is immobilized at 1 µg/mL (100 µL/well) can bind Human P-Cadherin. The ED50 for this effect is 1.046 μg/mL.

  • CDCP1 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for CDCP1 Protein, Human (HEK293, His)
  • Experimental Validation Results for CDCP1 Protein, Human (HEK293, His)
  • CDCP1 Protein, Human (HEK293, His) Product Image