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Chordin-like 1/CHRDL1 Protein, Human (N-His)

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The CHRDL1 protein acts as a BMP4 antagonist, blocking its receptor interaction and redirecting neural stem cells from gliogenesis to neurogenesis. The role of CHRDL1 in preventing glial cell fate adoption supports neuronal differentiation in the brain. Chordin-like 1/CHRDL1 Protein, Human (N-His) is the recombinant human-derived Chordin-like 1/CHRDL1 protein, expressed by E. coli , with N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Chordin-like 1/CHRDL1 Protein, Human (N-His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

The CHRDL1 protein acts as a BMP4 antagonist, blocking its receptor interaction and redirecting neural stem cells from gliogenesis to neurogenesis. The role of CHRDL1 in preventing glial cell fate adoption supports neuronal differentiation in the brain. Chordin-like 1/CHRDL1 Protein, Human (N-His) is the recombinant human-derived Chordin-like 1/CHRDL1 protein, expressed by E. coli , with N-His labeled tag.

Background

The Chordin-like 1 (CHRDL1) protein serves as an antagonist to BMP4 by binding to it, thus impeding its interaction with receptors. This interaction leads to a shift in the fate commitment of neural stem cells, steering them away from gliogenesis towards neurogenesis. CHRDL1's involvement in preventing the adoption of a glial fate contributes to the neuronal differentiation of neural stem cells in the brain. Beyond its role in neural development, CHRDL1 may play a crucial part in dorsoventral axis formation and embryonic bone development (By similarity). Additionally, CHRDL1 is implicated in regulating retinal angiogenesis by modulating BMP4 actions in endothelial cells. Its participation in anterior segment eye development underscores its multifaceted role in various developmental processes.

Verified Bioactivity

Immobilized Human CHRDL1, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CHRDL1 Antibody, hFc Tag with the EC50 of 9.4 ng/ml determined by ELISA.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-8*His
  • Accession

    Q9BU40-1 (E28-C456)

  • Gene ID
    91851
  • Molecular Construction
    • N-term
    • 8*His
    • CHRDL1 (E28-C456)
      Accession # Q9BU40-1
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    CHRDL1; Neuralin-1; Prev. MGC1; Megalocornea 1 (X-Linked); NRLN1; DA141H5.1; CHL; MGCN; Chordin-Like Protein 1; VOPT; Neurogenesin-1; Chordin Like 1; Ventroptin

  • AA Sequence

    EQVKHSETYCMFQDKKYRVGERWHPYLEPYGLVYCVNCICSENGNVLCSRVRCPNVHCLSPVHIPHLCCPRCPDSLPPVNNKVTSKSCEYNGTTYQHGELFVAEGLFQNRQPNQCTQCSCSEGNVYCGLKTCPKLTCAFPVSVPDSCCRVCRGDGELSWEHSDGDIFRQPANREARHSYHRSHYDPPPSRQAGGLSRFPGARSHRGALMDSQQASGTIVQIVINNKHKHGQVCVSNGKTYSHGESWHPNLRAFGIVECVLCTCNVTKQECKKIHCPNRYPCKYPQKIDGKCCKVCPGKKAKELPGQSFDNKGYFCGEETMPVYESVFMEDGETTRKIALETERPPQVEVHVWTIRKGILQHFHIEKISKRMFEELPHFKLVTRTTLSQWKIFTEGEAQISQMCSSRVCRTELEDLVKVLYLERSEKGHC

  • Predicted Molecular Mass

    50 kDa

  • Molecular Weight

    Approximately 50 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 4 mM HCl, pH 4.0, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

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