CNTN5/Contactin-5 Protein, Mouse (HEK293, His)

The CNTN5/Contactin-5 protein is a member of the contactin family and plays a crucial role in mediating cell surface interactions during nervous system development. Although it exhibits neurite growth-promoting activity in cerebral cortical neurons, it does not exhibit the same effect in hippocampal neurons, highlighting its cell type-specific function in neuronal growth. CNTN5/Contactin-5 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CNTN5/Contactin-5 protein, expressed by HEK293 , with C-His labeled tag. The total length of CNTN5/Contactin-5 Protein, Mouse (HEK293, His) is 1058 a.a., with molecular weight of 110-130 kDa.

Contactin-5 (CNTN5) is a protein crucial for mediating cell surface interactions during nervous system development, emphasizing its role in the establishment of neural connections. It exhibits neurite outgrowth-promoting activity, particularly in cerebral cortical neurons, highlighting its involvement in the extension of neuronal processes during brain development (By similarity). Interestingly, this neurite outgrowth-promoting activity is not observed in hippocampal neurons, suggesting a context-dependent function. CNTN5 is also implicated in neuronal activity within the auditory system, underlining its diverse roles in various regions of the nervous system. Furthermore, CNTN5 interacts with PTPRG, suggesting potential involvement in signaling pathways or cellular adhesion during neural development. The multifaceted functions and interactions of Contactin-5 underscore its importance in orchestrating complex processes during nervous system development.

Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells. When 5×10⁴ cells/well are added to CNTN5 coated plates (2.5μg/mL and 100 μL/well), approximately 51.30% will adhere specifically after 60 minutes at 37°C.

Mouse

HEK293

C-10*His

P68500 (L24-Q1058)

244682  [NCBI]

LSGLSTSYAALLRIKKSSTSPFGSKSRPRFSSPSLGTISVSPPSWRGAAQQYHSPGNLYHSSDAFRQDESVDYGPVFVQEPDDVIFPTDSDEKKVALNCEVRGNPSPSYRWLRNGTEIALESDYRYSLIDGTFIISNPSELRDSGLYQCLATNSFGSILSREATLQFAYLGNFSGRTRSAVSVREGQGVVLMCSPPPHSPEIIYSWVFNEFPSFVAEDSRRFISQETGNLYISKVQTSDVGSYICLVKNAVTNARVLSPPTPLTLRNDGVMGEYEPKIEVHFPFTVTAAKGTTVKMECFALGNPVPTITWMKVNGYIPSKSRLRKSQAVLEIPNLQLDDAGIYECTAENSRGKNSFRGQLQIFTYPHWVQKLNDTQLDSGSPLQWECKATGKPRPTYRWLKNGAPLLPQSRVDTVNGILAIQSVNQSDAGMYQCLAENKYGAIYASAELKILASPPSFELNQVKKSIIVTKDRGVLIECEPQGSPKPAISWRKGDKAVRANKRIAILPDGSLRILNASKADEGKYICQGVNIFGSAEIIASLSVKEPTRIELTPKRTELTVGESIVLNCKAIHDASLDVTFYWTLKGQPIDFEKEGGHFENIRAQASSADLMIRNILLMHAGRYGCRVQTTADSVSDEAELLVRGPPGPPGVVIVEEITESTATLSWSPATDNHSPISSYNLQARSPFSLGWQTVKTVPEVITGDMESAMAVDLNPWVEYEFRVVATNPIGTGDPSIPSRMIRTNEAVPKTAPSNVSGRSGRRHELVIAWEPVSEEFQNGEGFGYIVAFRPNGTRGWKEKMVTSSEASKFIYRDESVPPLTPFEVKVGVYNNKGDGPFSQIVVICSAEGEPTAAPTDVTATSVSVSEIFVVWKHVKESLGRPQGFEISYWKDTEPEDSVETVRTRGNESFVMLTGLEGNTLYHLTVRAYNGAGYGPPSREASTTTKRHPPREPPGNLRWEQQGSQVSLGWEPVRPLANESEVMGYKVFYRQEGHSEGQVIETQKPQAVVPLPEAGVYIIEVRAYSEGGDGTASSQ

Approximately 110-135 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.