Collagen alpha-1(II) chain/COL2A1 Protein, Human (HEK293, His)

COL2A1, encoding the alpha-1(II) chain of type II collagen, is specific to cartilaginous tissues, essential for embryonic skeletal development, linear growth, and cartilage resilience against compressive forces. As a fundamental component of homotrimers, COL2A1 maintains the structural integrity and function of cartilage, crucial for skeletal form and function during development and in resisting mechanical stress. Collagen alpha-1 (II) chain/COL2A1 Protein, Human (HEK293, His) is the recombinant human-derived Collagen alpha-1(II) chain/COL2A1 protein, expressed by HEK293 , with N-His labeled tag.

The COL2A1 protein, encoding the alpha-1(II) chain of type II collagen, exhibits specificity for cartilaginous tissues, playing a crucial role in normal embryonic skeletal development, linear growth, and the resilience of cartilage against compressive forces. As a fundamental component of homotrimers, COL2A1 contributes to the structural integrity and function of cartilage, highlighting its indispensable role in maintaining skeletal form and function throughout development and in resisting mechanical stress.

Measured by its binding ability in a functional ELISA. When Recombinant Human COL2A1 is present at 2 μg/mL, can bind anti-COL2A1 Antibody.The ED50 for this effect is 188.4 ng/mL.

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  Measured by its binding ability in a functional ELISA. When Recombinant Human COL2A1 is present at 2 μg/mL, can bind anti-Collagen II Antibody.The ED₅₀ for this effect is 188.4 ng/mL.

Human

HEK293

N-His

P02458-2 (D1242-L1487)

1280  [NCBI]

Partial

DQAAGGLRQHDAEVDATLKSLNNQIESIRSPEGSRKNPARTCRDLKLCHPEWKSGDYWIDPNQGCTLDAMKVFCNMETGETCVYPNPANVPKKNWWSSKSKEKKHIWFGETINGGFHFSYGDDNLAPNTANVQMTFLRLLSTEGSQNITYHCKNSIAYLDEAAGNLKKALLIQGSNDVEIRAEGNSRFTYTALKDGCTKHTGKWGKTVIEYRSQKTSRLPIIDIAPMDIGGPEQEFGVDIGPVCFL

Approximately 32-37 kDa

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.