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Collagen alpha-1(II) chain/COL2A1 Protein, Human (HEK293, His)

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Based on 1 publication(s) in Google Scholar

COL2A1, encoding the alpha-1(II) chain of type II collagen, is specific to cartilaginous tissues, essential for embryonic skeletal development, linear growth, and cartilage resilience against compressive forces. As a fundamental component of homotrimers, COL2A1 maintains the structural integrity and function of cartilage, crucial for skeletal form and function during development and in resisting mechanical stress. Collagen alpha-1 (II) chain/COL2A1 Protein, Human (HEK293, His) is the recombinant human-derived Collagen alpha-1(II) chain/COL2A1 protein, expressed by HEK293 , with N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Collagen alpha-1(II) chain/COL2A1 Protein, Human (HEK293, His)
  • Experimental Validation Results for Collagen alpha-1(II) chain/COL2A1 Protein, Human (HEK293, His)
  • Collagen alpha-1(II) chain/COL2A1 Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Use of MCE Products

Biological Activity

Description

COL2A1, encoding the alpha-1(II) chain of type II collagen, is specific to cartilaginous tissues, essential for embryonic skeletal development, linear growth, and cartilage resilience against compressive forces. As a fundamental component of homotrimers, COL2A1 maintains the structural integrity and function of cartilage, crucial for skeletal form and function during development and in resisting mechanical stress. Collagen alpha-1 (II) chain/COL2A1 Protein, Human (HEK293, His) is the recombinant human-derived Collagen alpha-1(II) chain/COL2A1 protein, expressed by HEK293 , with N-His labeled tag.

Background

The COL2A1 protein, encoding the alpha-1(II) chain of type II collagen, exhibits specificity for cartilaginous tissues, playing a crucial role in normal embryonic skeletal development, linear growth, and the resilience of cartilage against compressive forces. As a fundamental component of homotrimers, COL2A1 contributes to the structural integrity and function of cartilage, highlighting its indispensable role in maintaining skeletal form and function throughout development and in resisting mechanical stress.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Human COL2A1 is present at 2 μg/mL, can bind anti-COL2A1 Antibody.The ED50 for this effect is 188.4 ng/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. When Recombinant Human COL2A1 is present at 2 μg/mL, can bind anti-Collagen II Antibody.The ED50 for this effect is 188.4 ng/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag N-His
  • Accession

    P02458-2 (D1242-L1487)

  • Gene ID
    1280  [NCBI]
  • Molecular Construction
    • N-term
    • His
    • COL2A1 (D1242-L1487)
      Accession # P02458-2
    • C-term
  • Protein Length

    Full Length of Chondrocalcin Chain

  • Synonyms

    COL2A1; Alpha-1 Collagen Type II; Prev. SEDC; Chondrocalcin; Prev. AOM; SEMDSTWK; STL1; COL11A3; Arthroophthalmopathy, Progressive (Stickler Syndrome); SEDSTN; Collagen, Type II, Alpha 1; SMDALG; Collagen Alpha-1(II) Chain; ANFH1; Alpha-1 Type II Collagen

  • AA Sequence

    DQAAGGLRQHDAEVDATLKSLNNQIESIRSPEGSRKNPARTCRDLKLCHPEWKSGDYWIDPNQGCTLDAMKVFCNMETGETCVYPNPANVPKKNWWSSKSKEKKHIWFGETINGGFHFSYGDDNLAPNTANVQMTFLRLLSTEGSQNITYHCKNSIAYLDEAAGNLKKALLIQGSNDVEIRAEGNSRFTYTALKDGCTKHTGKWGKTVIEYRSQKTSRLPIIDIAPMDIGGPEQEFGVDIGPVCFL

  • Molecular Weight

    Approximately 37 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. When Recombinant Human COL2A1 is present at 2 μg/mL, can bind anti-Collagen II Antibody.The ED50 for this effect is 188.4 ng/mL.

  • Collagen alpha-1(II) chain/COL2A1 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Collagen alpha-1(II) chain/COL2A1 Protein, Human (HEK293, His)
  • Experimental Validation Results for Collagen alpha-1(II) chain/COL2A1 Protein, Human (HEK293, His)
  • Collagen alpha-1(II) chain/COL2A1 Protein, Human (HEK293, His) Product Image