Copine-1/CPNE1 Protein, Human (His)
Based on 1 Customer Validation
Copine-1/CPNE1 protein is a calcium-dependent phospholipid-binding protein that participates in the TNF-α receptor signaling pathway in a calcium-dependent manner. CPNE1 induces AKT-dependent neurite outgrowth and is also involved in TNF-α-induced repression of NF-κ-B transcription. Copine-1/CPNE1 Protein, Human (His) is the recombinant human-derived Copine-1/CPNE1 protein, expressed by E. coli , with N-6*His, C-6*His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Copine-1/CPNE1 protein is a calcium-dependent phospholipid-binding protein that participates in the TNF-α receptor signaling pathway in a calcium-dependent manner. CPNE1 induces AKT-dependent neurite outgrowth and is also involved in TNF-α-induced repression of NF-κ-B transcription. Copine-1/CPNE1 Protein, Human (His) is the recombinant human-derived Copine-1/CPNE1 protein, expressed by E. coli , with N-6*His, C-6*His labeled tag.
Background
Copine-1, also known as CPNE1, is a calcium-dependent phospholipid-binding protein that actively participates in calcium-mediated intracellular processes. Involved in the TNF-alpha receptor signaling pathway, Copine-1 exhibits calcium-dependent phospholipid binding properties. Additionally, it plays a pivotal role in neuronal progenitor cell differentiation by inducing neurite outgrowth through an AKT-dependent signaling cascade, operating in a calcium-independent manner. Copine-1 may also contribute to the recruitment of target proteins to the cell membrane in a calcium-dependent fashion and is implicated in membrane trafficking. Furthermore, it is involved in TNF-alpha-induced NF-kappa-B transcriptional repression by inducing endoprotease processing of various NF-kappa-B subunits, including p65/RELA, p50/NFKB1, p52/NFKB2, RELB, and REL. The multifaceted functions of Copine-1 highlight its versatile role in cellular processes, ranging from neuronal development to membrane dynamics and signal transduction pathways.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-6*His;C-6*His
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Accession
Q99829 (M1-A537)
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Molecular Construction
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N-term
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6*His
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Copine-1 (M1-A537)
Accession # Q99829 -
6*His
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C-term
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Protein Length
Full Length
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Synonyms
CPNE1; Copine-1; Copine 1; Chromobindin 17; Copine I; COPN1; CPN1
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AA Sequence
MAHCVTLVQLSISCDHLIDKDIGSKSDPLCVLLQDVGGGSWAELGRTERVRNCSSPEFSKTLQLEYRFETVQKLRFGIYDIDNKTPELRDDDFLGGAECSLGQIVSSQVLTLPLMLKPGKPAGRGTITVSAQELKDNRVVTMEVEARNLDKKDFLGKSDPFLEFFRQGDGKWHLVYRSEVIKNNLNPTWKRFSVPVQHFCGGNPSTPIQVQCSDYDSDGSHDLIGTFHTSLAQLQAVPAEFECIHPEKQQKKKSYKNSGTIRVKICRVETEYSFLDYVMGGCQINFTVGVDFTGSNGDPSSPDSLHYLSPTGVNEYLMALWSVGSVVQDYDSDKLFPAFGFGAQVPPDWQVSHEFALNFNPSNPYCAGIQGIVDAYRQALPQVRLYGPTNFAPIINHVARFAAQAAHQGTASQYFMLLLLTDGAVTDVEATREAVVRASNLPMSVIIVGVGGADFEAMEQLDADGGPLHTRSGQAAARDIVQFVPYRRFQNAPREALAQTVLAEVPTQLVSYFRAQGWAPLKPLPPSAKDPAQAPQA
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Predicted Molecular Mass
62.3 kDa
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Molecular Weight
Approximately 58-66 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 20 mM PB，150 mM NaCl, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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