CSF1R Protein, Human (HEK293, hFc, SPR verified)
Based on 1 Customer Validation
CSF1R protein is ubiquitously found in mononuclear phagocytes, especially macrophages and monocytes, and is involved in a variety of biological processes. It induces the release of proinflammatory chemokines in response to IL34 and CSF1, contributing to innate immunity and inflammation. CSF1R Protein, Human (HEK293, hFc) is the recombinant human-derived CSF1R protein, expressed by HEK293 , with C-hFc labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
CSF1R protein is ubiquitously found in mononuclear phagocytes, especially macrophages and monocytes, and is involved in a variety of biological processes. It induces the release of proinflammatory chemokines in response to IL34 and CSF1, contributing to innate immunity and inflammation. CSF1R Protein, Human (HEK293, hFc) is the recombinant human-derived CSF1R protein, expressed by HEK293 , with C-hFc labeled tag.
Background
The CSF1R protein, particularly found in mononuclear phagocytes like macrophages and monocytes, is involved in various biological processes. It promotes the release of pro-inflammatory chemokines in response to IL34 and CSF1, contributing to innate immunity and inflammatory processes. Additionally, CSF1R is crucial for regulating osteoclast proliferation and differentiation, bone resorption, normal bone and tooth development. It is also required for normal fertility in both males and females, as well as for the development of milk ducts and acinar structures in the mammary gland during pregnancy.
Verified Bioactivity
1.This product does not contain protein kinase domain.
2.Immobilized Human M-CSF Protein at 5 μg/mL (100 μL/well) can bind Human M-CSF R Protein. The ED50 for this effect is 26.94 ng/mL.
3.Immobilized Human M-CSF R Protein at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-34 Protein. The ED50 for this effect is 14.96 ng/mL.
4.Human Human M-CSF R captured on CM5 Chip can bind M-CSF Protein with an affinity constant of 0.49 nM as determined in SPR assay. (Biacore 8K)
5.Human M-CSF R Protein captured on CM5 Chip can bind Biotinylated Human IL-34 Protein with an affinity constant of 44 nM as determined in SPR assay. (Biacore 8K)
Technical Parameters
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Species Human
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Source HEK293
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Tag C-hFc
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Accession
P07333-1 (I20-E512)
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Gene ID1436
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Molecular Construction
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N-term
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CSF1R (I20-E512)
Accession # P07333-1 -
hFc
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C-term
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Protein Length
Partial Extracellular Domain
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Synonyms
Macrophage colony-stimulating factor 1 receptor; CSF-1R; M-CSF-R; CD115; CSF1R; FMS
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AA Sequence
IPVIEPSVPELVVKPGATVTLRCVGNGSVEWDGPPSPHWTLYSDGSSSILSTNNATFQNTGTYRCTEPGDPLGGSAAIHLYVKDPARPWNVLAQEVVVFEDQDALLPCLLTDPVLEAGVSLVRVRGRPLMRHTNYSFSPWHGFTIHRAKFIQSQDYQCSALMGGRKVMSISIRLKVQKVIPGPPALTLVPAELVRIRGEAAQIVCSASSVDVNFDVFLQHNNTKLAIPQQSDFHNNRYQKVLTLNLDQVDFQHAGNYSCVASNVQGKHSTSMFFRVVESAYLNLSSEQNLIQEVTVGEGLNLKVMVEAYPGLQGFNWTYLGPFSDHQPEPKLANATTKDTYRHTFTLSLPRLKPSEAGRYSFLARNPGGWRALTFELTLRYPPEVSVIWTFINGSGTLLCAASGYPQPNVTWLQCSGHTDRCDEAQVLQVWDDPYPEVLSQEPFHKVTVQSLLTVETLEHNQTYECRAHNSVGSGSWAFIPISAGAHTHPPDE
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Predicted Molecular Mass
80.6 kDa
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Molecular Weight
Approximately 95-130 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.; ≥ 95%, as determined by HPLC.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
<0.01 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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