DDR1 Protein, Human (Biotinylated, HEK293, His-Avi)
The DDR1 protein is a tyrosine kinase and cell surface receptor for fibrillar collagen that tightly regulates cell attachment and affects extracellular matrix processes. Upon collagen binding, DDR1 initiates a signaling cascade involving SRC, activating MAP kinase. DDR1, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived DDR1 protein, expressed by HEK293, with C-Avi;C-hFc labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The DDR1 protein is a tyrosine kinase and cell surface receptor for fibrillar collagen that tightly regulates cell attachment and affects extracellular matrix processes. Upon collagen binding, DDR1 initiates a signaling cascade involving SRC, activating MAP kinase. DDR1, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived DDR1 protein, expressed by HEK293, with C-Avi;C-hFc labeled tag.
Background
DDR1 protein serves as a tyrosine kinase and functions as a cell surface receptor for fibrillar collagen, playing a pivotal role in regulating cell attachment to the extracellular matrix and influencing processes such as extracellular matrix remodeling, cell migration, differentiation, survival, and proliferation. Upon collagen binding, DDR1 initiates a signaling cascade involving SRC and activates MAP kinases. This activation, in turn, leads to the up-regulation of matrix metalloproteinases MMP2, MMP7, and MMP9, facilitating extracellular matrix remodeling and promoting cell migration and wound healing. DDR1's significance extends to various physiological processes, including normal blastocyst implantation during pregnancy, mammary gland differentiation, lactation, and the maintenance of ear morphology and hearing. Additionally, DDR1 contributes to arterial wound healing by promoting smooth muscle cell migration and plays a role in tumor cell invasion, further underscoring its diverse functional repertoire. Notably, DDR1 phosphorylates PTPN11 as part of its regulatory activities.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-Avi;C-His
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Accession
Q08345-1 (D21-A417)
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Gene ID780
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Molecular Construction
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N-term
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DDR1 (D21-A417)
Accession # Q08345-1 -
His-Avi
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C-term
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Protein Length
Extracellular Domain
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Conjugation
Biotin
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Synonyms
DDR1; HGK2; Prev. EDDR1; Neurotrophic Tyrosine Kinase, Receptor, Type 4; Prev. NTRK4; Discoidin Domain Receptor Family, Member 1; Prev. PTK3A; Epithelial Discoidin Domain Receptor 1; Prev. CAK; PTK3A Protein Tyrosine Kinase 3A; Prev. NEP; Receptor Protein-Tyrosine Kinase; RTK6; Neuroepithelial Tyrosine Kinase; Epithelial Discoidin Domain-Containing Receptor 1; Discoidin Domain Receptor DDR1e; CD167 Antigen-Like Family Member A; Discoidin Domain Receptor DDR1d; Tyrosine Kinase DDR; Protein-Tyrosine Kinase 3A; CD167; CD167a Antigen; Discoidin Receptor Tyrosine Kinase; MCK-10; Protein-Tyrosine Kinase RTK-6; MCK10; Mammary Carcinoma Kinase 10; TRK E; Tyrosine-Protein Kinase CAK; PTK3; Cell Adhesion Kinase; DDR; Discoidin Domain Receptor Tyrosine Kinase 1; TRKE
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AA Sequence
DMKGHFDPAKCRYALGMQDRTIPDSDISASSSWSDSTAARHSRLESSDGDGAWCPAGSVFPKEEEYLQVDLQRLHLVALVGTQGRHAGGLGKEFSRSYRLRYSRDGRRWMGWKDRWGQEVISGNEDPEGVVLKDLGPPMVARLVRFYPRADRVMSVCLRVELYGCLWRDGLLSYTAPVGQTMYLSEAVYLNDSTYDGHTVGGLQYGGLGQLADGVVGLDDFRKSQELRVWPGYDYVGWSNHSFSSGYVEMEFEFDRLRAFQAMQVHCNNMHTLGARLPGGVECRFRRGPAMAWEGEPMRHNLGGNLGDPRARAVSVPLGGRVARFLQCRFLFAGPWLLFSEISFISDVVNNSSPALGGTFPPAPWWPPGPPPTNFSSLELEPRGQQPVAKAEGSPTA
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Predicted Molecular Mass
47.0 kDa.
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Molecular Weight
Approximately 60-75 kDa, based on Bis-Tris PAGEunder reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by Bis-Tris PAGE.
Product Properties
Lyophilized powder.
< 1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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