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DEC-205/CD205 Protein, Human (HEK293, His)

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Based on 1 Customer Validation

DEC-205/CD205 Protein, an endocytic receptor, transports captured antigens to a specialized processing compartment, placing it at the forefront of antigen presentation. This role is crucial for immune surveillance and initiating immune responses. DEC-205/CD205's involvement also downregulates B-lymphocyte proliferation, showcasing its regulatory impact on immune cell dynamics and responses. DEC-205/CD205 Protein, Human (HEK293, His) is the recombinant human-derived DEC-205/CD205 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for DEC-205/CD205 Protein, Human (HEK293, His)
  • Experimental Validation Results for DEC-205/CD205 Protein, Human (HEK293, His)
  • DEC-205/CD205 Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

DEC-205/CD205 Protein, an endocytic receptor, transports captured antigens to a specialized processing compartment, placing it at the forefront of antigen presentation. This role is crucial for immune surveillance and initiating immune responses. DEC-205/CD205's involvement also downregulates B-lymphocyte proliferation, showcasing its regulatory impact on immune cell dynamics and responses. DEC-205/CD205 Protein, Human (HEK293, His) is the recombinant human-derived DEC-205/CD205 protein, expressed by HEK293 , with C-His labeled tag.

Background

DEC-205/CD205 protein serves as an endocytic receptor, facilitating the transportation of captured antigens from the extracellular space to a specialized antigen-processing compartment. This function positions DEC-205/CD205 at the forefront of antigen presentation, playing a crucial role in immune surveillance and the initiation of immune responses. Moreover, its involvement leads to the downregulation of B-lymphocyte proliferation, highlighting its regulatory impact on immune cell dynamics and responses.

Verified Bioactivity

Immobilized Recombinant Rat CD302 Protein Protein at 5 µg/mL (100 µL/well) can bind Biotinylated Human CD205 protein. The ED50 for this effect is 4.075 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Immobilized Recombinant Rat CD302 Protein Protein at 5 µg/mL (100 µL/well) can bind Biotinylated Human CD205 protein. The ED50 for this effect is 4.075 μg/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-10*His
  • Accession

    O60449-1/NP_002340.2 (A32-G1664)

  • Gene ID
    4065  [NCBI]
  • Molecular Construction
    • N-term
    • CD205 (A32-G1664)
      Accession # O60449-1/NP_002340.2
    • His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    LY75; Alternative Protein LY75; Lymphocyte Antigen 75; CD205 Antigen; DEC-205; GP200-MR6; CLEC13B; Gp200-MR6; CD205; LY-75; C-Type Lectin Domain Family 13 Member B; Ly-75

  • AA Sequence

    ANDPFTIVHGNTGKCIKPVYGWIVADDCDETEDKLWKWVSQHRLFHLHSQKCLGLDITKSVNELRMFSCDSSAMLWWKCEHHSLYGAARYRLALKDGHGTAISNASDVWKKGGSEESLCDQPYHEIYTRDGNSYGRPCEFPFLIDGTWHHDCILDEDHSGPWCATTLNYEYDRKWGICLKPENGCEDNWEKNEQFGSCYQFNTQTALSWKEAYVSCQNQGADLLSINSAAELTYLKEKEGIAKIFWIGLNQLYSARGWEWSDHKPLNFLNWDPDRPSAPTIGGSSCARMDAESGLWQSFSCEAQLPYVCRKPLNNTVELTDVWTYSDTRCDAGWLPNNGFCYLLVNESNSWDKAHAKCKAFSSDLISIHSLADVEVVVTKLHNEDIKEEVWIGLKNINIPTLFQWSDGTEVTLTYWDENEPNVPYNKTPNCVSYLGELGQWKVQSCEEKLKYVCKRKGEKLNDASSDKMCPPDEGWKRHGETCYKIYEDEVPFGTNCNLTITSRFEQEYLNDLMKKYDKSLRKYFWTGLRDVDSCGEYNWATVGGRRRAVTFSNWNFLEPASPGGCVAMSTGKSVGKWEVKDCRSFKALSICKKMSGPLGPEEASPKPDDPCPEGWQSFPASLSCYKVFHAERIVRKRNWEEAERFCQALGAHLSSFSHVDEIKEFLHFLTDQFSGQHWLWIGLNKRSPDLQGSWQWSDRTPVSTIIMPNEFQQDYDIRDCAAVKVFHRPWRRGWHFYDDREFIYLRPFACDTKLEWVCQIPKGRTPKTPDWYNPDRAGIHGPPLIIEGSEYWFVADLHLNYEEAVLYCASNHSFLATITSFVGLKAIKNKIANISGDGQKWWIRISEWPIDDHFTYSRYPWHRFPVTFGEECLYMSAKTWLIDLGKPTDCSTKLPFICEKYNVSSLEKYSPDSAAKVQCSEQWIPFQNKCFLKIKPVSLTFSQASDTCHSYGGTLPSVLSQIEQDFITSLLPDMEATLWIGLRWTAYEKINKWTDNRELTYSNFHPLLVSGRLRIPENFFEEESRYHCALILNLQKSPFTGTWNFTSCSERHFVSLCQKYSEVKSRQTLQNASETVKYLNNLYKIIPKTLTWHSAKRECLKSNMQLVSITDPYQQAFLSVQALLHNSSLWIGLFSQDDELNFGWSDGKRLHFSRWAETNGQLEDCVVLDTDGFWKTVDCNDNQPGAICYYSGNETEKEVKPVDSVKCPSPVLNTPWIPFQNCCYNFIITKNRHMATTQDEVHTKCQKLNPKSHILSIRDEKENNFVLEQLLYFNYMASWVMLGITYRNKSLMWFDKTPLSYTHWRAGRPTIKNEKFLAGLSTDGFWDIQTFKVIEEAVYFHQHSILACKIEMVDYKEEYNTTLPQFMPYEDGIYSVIQKKVTWYEALNMCSQSGGHLASVHNQNGQLFLEDIVKRDGFPLWVGLSSHDGSESSFEWSDGSTFDYIPWKGQTSPGNCVLLDPKGTWKHEKCNSVKDGAICYKPTKSKKLSRLTYSSRCPAAKENGSRWIQYKGHCYKSDQALHSFSEAKKLCSKHDHSATIVSIKDEDENKFVSRLMRENNNITMRVWLGLSQHSVDQSWSWLDGSEVTFVKWENKSKSGVGRCSMLIASNETWKKVECEHGFGRVVCKVPLG

  • Molecular Weight

    Approximately 190 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Immobilized Recombinant Rat CD302 Protein Protein at 5 µg/mL (100 µL/well) can bind Biotinylated Human CD205 protein. The ED50 for this effect is 4.075 μg/mL.

  • DEC-205/CD205 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for DEC-205/CD205 Protein, Human (HEK293, His)
  • Experimental Validation Results for DEC-205/CD205 Protein, Human (HEK293, His)
  • DEC-205/CD205 Protein, Human (HEK293, His) Product Image