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  3. DKK-1 Protein, Rat (HEK293, His)

DKK-1 Protein, Rat (HEK293, His)

Cat. No.: HY-P73305
COA Handling Instructions

For research use only. We do not sell to patients.

Size Price Stock Quantity
Free Sample   Apply now  
5 μg $88 In-stock
10 μg $150 In-stock
50 μg $420 In-stock
100 μg   Get quote  
250 μg   Get quote  

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Publications Citing Use of MCE DKK-1 Protein, Rat (HEK293, His)

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Biological Activity

Measured by its ability to inhibit Wnt3a induced alkaline phosphatase production by C3H10 T1/2 cells and the ED50 for this effect is typically 0.2 μg/mL.

Species

Rat

Source

HEK293

Tag

C-His

Accession

NP_001099820.1 (M1-H270)

Gene ID

293897  [NCBI]

Synonyms
dickkopf WNT signaling pathway inhibitor 1; Dickkopf-1; dickkopf-related protein 1; Dkk1; Dkk-1; hDkk-1
AA Sequence

MTVVRAVAAVRFLVVLSTMALCSLPPLGVSATLNSVLINSNAIKNLPPPLGGAGGQPGSAVSVAPGVLYEGGNKYQTLDNYQPYPCAEDEECGTDEYCSSPSRGAAGVGGVQICLACRKRRKRCMRHAMCCPGNYCKNGICMPSDHSHLPRGEIEEGIIENLGNDHGAGDGYPRRTTLTSKIYHTKGQEGSVCLRSSDCATGLCCARHFWSKICKPVLKEGQVCTKHRRKGSHGLEIFQRCYCGEGLACRIQKDHHQTSNSSRLHTCQRH

Molecular Weight

Approximately 27.4 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

DKK-1 Protein, Rat (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
DKK-1 Protein, Rat (HEK293, His)
Cat. No.:
HY-P73305
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