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DKK-3 Protein, Mouse (HEK293, His)

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Based on 1 Customer Validation

DKK-3 protein antagonizes canonical Wnt signaling by inhibiting LRP5/6-Wnt interaction. It forms a ternary complex with KREMEN and promotes LRP5/6 internalization. DKK-3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived DKK-3 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for DKK-3 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for DKK-3 Protein, Mouse (HEK293, His)
  • DKK-3 Protein, Mouse (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

DKK-3 protein antagonizes canonical Wnt signaling by inhibiting LRP5/6-Wnt interaction. It forms a ternary complex with KREMEN and promotes LRP5/6 internalization. DKK-3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived DKK-3 protein, expressed by HEK293 , with C-His labeled tag.

Background

The DKK-3 protein is involved in antagonizing canonical Wnt signaling by inhibiting the interaction between LRP5/6 and Wnt. It achieves this through the formation of a ternary complex with the transmembrane protein KREMEN, which facilitates the internalization of LRP5/6. DKKs, including DKK-3, are crucial in vertebrate development as they locally inhibit Wnt-regulated processes such as antero-posterior axial patterning, limb development, somitogenesis, and eye formation. In adult organisms, DKKs are implicated in bone formation, bone diseases, cancer, and Alzheimer's disease. Additionally, DKK-3 interacts with LRP5 and LRP6 proteins. It is important to note that DKK-3 does not possess carboxypeptidase activity or dipeptidyl-peptidase IV type activity.

Verified Bioactivity

Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. The ED50 for this effect is 4.628 μg/mL, corresponding to a specific activity is 216.076 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. The ED50 for this effect is 4.628 μg/mL, corresponding to a specific activity is 216.076 units/mg.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q9QUN9 (P23-I349)

  • Gene ID
    50781  [NCBI]
  • Molecular Construction
    • N-term
    • DKK-3 (P23-I349)
      Accession # Q9QUN9
    • His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    DKK3; HDkk-3; Dickkopf Wnt Signaling Pathway Inhibitor 3; Dkk-3; REIC; Dickkopf WNT Signaling Pathway Inhibitor 3; Dickkopf-Related Protein 3; Dickkopf (Xenopus Laevis) Homolog 3; CRRL; Dickkopf 3 Homolog (Xenopus Laevis); RIG; Dickkopf 3 Homolog; Cardiom

  • AA Sequence

    PSPTVTWTPAEPGPALNYPQEEATLNEMFREVEELMEDTQHKLRSAVEEMEAEEAAAKTSSEVNLASLPPNYHNETSTETRVGNNTVHVHQEVHKITNNQSGQVVFSETVITSVGDEEGKRSHECIIDEDCGPTRYCQFSSFKYTCQPCRDQQMLCTRDSECCGDQLCAWGHCTQKATKGGNGTICDNQRDCQPGLCCAFQRGLLFPVCTPLPVEGELCHDPTSQLLDLITWELEPEGALDRCPCASGLLCQPHSHSLVYMCKPAFVGSHDHSEESQLPREAPDEYEDVGFIGEVRQELEDLERSLAQEMAFEGPAPVESLGGEEEI

  • Molecular Weight

    Approximately 55-66 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. The ED50 for this effect is 4.628 μg/mL, corresponding to a specific activity is 216.076 units/mg.

  • DKK-3 Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for DKK-3 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for DKK-3 Protein, Mouse (HEK293, His)
  • DKK-3 Protein, Mouse (HEK293, His) Product Image