eIF3K Protein, Human (sf9, His-GST)

The eIF3K protein is an important component of the eukaryotic translation initiation factor 3 (eIF-3) complex and plays a crucial role in various steps of protein synthesis initiation. It stimulates recruitment of mRNA to the 43S preinitiation complex (43S PIC) and promotes AUG recognition scanning. eIF3K Protein, Human (sf9, His-GST) is the recombinant human-derived eIF3K protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of eIF3K Protein, Human (sf9, His-GST) is 217 a.a., with molecular weight of ~47 kDa.

eIF3K protein is an integral component of the eukaryotic translation initiation factor 3 (eIF-3) complex, a crucial assembly required for various steps in protein synthesis initiation. This complex associates with the 40S ribosome, facilitating the recruitment of essential factors to form the 43S pre-initiation complex (43S PIC). eIF-3K within the eIF-3 complex stimulates mRNA recruitment to the 43S PIC and assists in scanning the mRNA for AUG recognition. Furthermore, the eIF-3 complex plays a pivotal role in disassembling and recycling post-termination ribosomal complexes, preventing premature joining of the 40S and 60S ribosomal subunits before initiation. Notably, eIF-3K contributes to the translation initiation of specific mRNAs involved in cell proliferation, including those related to cell cycling, differentiation, and apoptosis. Through different modes of RNA stem-loop binding, eIF-3K can exert either translational activation or repression. The eIF-3 complex, to which eIF-3K belongs, comprises 13 subunits and is organized into three stable modules (A, B, and C), with eIF-3K situated in module C. Interactions with other subunits, such as EIF3J and CCND3, highlight the intricate regulatory network in which eIF3K is involved, providing further insights into its multifaceted role in translation initiation and cellular processes.

Human

Sf9 insect cells

N-His;N-GST

Q9UBQ5 (A2-Q218)

27335  [NCBI]

Approximately 47 kDa

Greater than 90% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM Nacl, 3 mM DTT, pH 7.4, 10% Glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.