EP300 Protein, Human (379a.a, His)
Based on 1 Customer Validation
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Technical Parameters
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Species Human
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Source E. coli
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Tag C-6*His
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Accession
Q09472 (N1286-D1664)
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Gene ID2033
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Molecular Construction
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N-term
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EP300 (N1286-D1664)
Accession # Q09472 -
6*His
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C-term
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Protein Length
Full Length of CBP/p300-type HAT Domain
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Synonyms
EP300; Histone Crotonyltransferase P300; EP300 Lysine Acetyltransferase; Histone Butyryltransferase P300; P300; Protein Lactyltransferas P300; Histone Acetyltransferase P300; E1A-Associated Protein P300; E1A Binding Protein P300; P300 HAT; KAT3B; E1A-Binding Protein, 300kD; Protein 2-Hydroxyisobutyryltransferase P300; Histone Acetyltransferase; Protein Isonicotinyltransferase P300; MKHK2; Protein Propionyltransferase P300; RSTS2
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AA Sequence
NKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHASDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPPPNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDYIFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFEGDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLSRGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDLMDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD
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Predicted Molecular Mass
44.58 kDa
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Molecular Weight
Approximately 48 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)