EXO I Protein, E. coli (His)

EXO I Protein, documented with high processivity, degrades single-stranded DNA (ssDNA). It also acts as a DNA deoxyribophosphodiesterase, releasing deoxyribose-phosphate moieties post-cleavage at apurinic/apyrimidinic (AP) sites by AP endonuclease or AP lyase. EXO I's dual functionality in ssDNA degradation and deoxyribose-phosphate removal at AP sites underscores its crucial role in DNA repair processes, maintaining genome integrity. EXO I Protein, E. coli (His) is the recombinant E. coli-derived EXO I protein, expressed by E. coli , with N-6*His labeled tag. EXO I Protein, E. coli (His), has molecular weight of ~54.5 kDa.

EXO I Protein, as the subject, exhibits a high processivity in the degradation of single-stranded DNA (ssDNA), as documented in various studies. Additionally, it functions as a DNA deoxyribophosphodiesterase, playing a role in the release of deoxyribose-phosphate moieties. This activity occurs following the cleavage of DNA at an apurinic/apyrimidinic (AP) site, catalyzed by either an AP endonuclease or AP lyase. The dual functionality of EXO I in ssDNA degradation and the removal of deoxyribose-phosphate moieties at AP sites reflects its crucial role in DNA repair processes and maintenance of genome integrity.

E.coli

E. coli

N-6*His

P04995

946529  [NCBI]

MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNIFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGLPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLVQQYADDKEKVALLKALWQYAEEIV

Approximately 54.5 kDa

≥ 95%, as determined by reducing SDS-PAGE.