EXO I Protein, E. coli (His)
EXO I Protein, documented with high processivity, degrades single-stranded DNA (ssDNA). It also acts as a DNA deoxyribophosphodiesterase, releasing deoxyribose-phosphate moieties post-cleavage at apurinic/apyrimidinic (AP) sites by AP endonuclease or AP lyase. EXO I's dual functionality in ssDNA degradation and deoxyribose-phosphate removal at AP sites underscores its crucial role in DNA repair processes, maintaining genome integrity. EXO I Protein, E. coli (His) is the recombinant E. coli-derived EXO I protein, expressed by E. coli , with N-6*His labeled tag. EXO I Protein, E. coli (His), has molecular weight of ~54.5 kDa.
- Species: E.coli
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
EXO I Protein, documented with high processivity, degrades single-stranded DNA (ssDNA). It also acts as a DNA deoxyribophosphodiesterase, releasing deoxyribose-phosphate moieties post-cleavage at apurinic/apyrimidinic (AP) sites by AP endonuclease or AP lyase. EXO I's dual functionality in ssDNA degradation and deoxyribose-phosphate removal at AP sites underscores its crucial role in DNA repair processes, maintaining genome integrity. EXO I Protein, E. coli (His) is the recombinant E. coli-derived EXO I protein, expressed by E. coli , with N-6*His labeled tag. EXO I Protein, E. coli (His), has molecular weight of ~54.5 kDa.
EXO I Protein, as the subject, exhibits a high processivity in the degradation of single-stranded DNA (ssDNA), as documented in various studies. Additionally, it functions as a DNA deoxyribophosphodiesterase, playing a role in the release of deoxyribose-phosphate moieties. This activity occurs following the cleavage of DNA at an apurinic/apyrimidinic (AP) site, catalyzed by either an AP endonuclease or AP lyase. The dual functionality of EXO I in ssDNA degradation and the removal of deoxyribose-phosphate moieties at AP sites reflects its crucial role in DNA repair processes and maintenance of genome integrity.
Technical Parameters
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Species E.coli
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Source E. coli
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Tag N-6*His
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Accession
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Gene ID946529 [NCBI]
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Molecular Construction
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N-term
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6*His
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EXO I
Accession # P04995 -
C-term
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Synonyms
Exodeoxyribonuclease I; EC:3.1.11.1; ExoI; DNA deoxyribophosphodiesterase; dRPase; Exonuclease I; sbcB; cpeA; xonA; b2011; JW1993
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AA Sequence
MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNIFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGLPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLVQQYADDKEKVALLKALWQYAEEIV
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Molecular Weight
Approximately 55 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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