FDH Protein, Pseudomonas sp.
Based on 1 Customer Validation
FDH Protein, Pseudomonas sp. is the recombinant FDH, expressed by E. coli , with tag Free labeled tag.
- Species: Others
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Biological Activity
Description
FDH Protein, Pseudomonas sp. is the recombinant FDH, expressed by E. coli , with tag Free labeled tag.
Background
FDH is an enzyme that catalyzes the NAD(+)-dependent oxidation of formate to carbon dioxide. In methylotrophic microorganisms, formate oxidation represents the final step in the methanol oxidation pathway. In non-methylotrophic organisms, FDH also plays a role in the detoxification of exogenous formate.
Technical Parameters
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Species Others
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Source E. coli
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Tag Tag Free
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Accession
P33160 (A2-V401)
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Gene ID/
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Protein Length
Full Length
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Synonyms
Formate dehydrogenase; EC:1.17.1.9; FDH; NAD-dependent formate dehydrogenase
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AA Sequence
AKVLCVLYDDPVDGYPKTYARDDLPKIDHYPGGQTLPTPKAIDFTPGQLLGSVSGELGLRKYLESNGHTLVVTSDKDGPDSVFERELVDADVVISQPFWPAYLTPERIAKAKNLKLALTAGIGSDHVDLQSAIDRNVTVAEVTYCNSISVAEHVVMMILSLVRNYLPSHEWARKGGWNIADCVSHAYDLEAMHVGTVAAGRIGLAVLRRLAPFDVHLHYTDRHRLPESVEKELNLTWHATREDMYPVCDVVTLNCPLHPETEHMINDETLKLFKRGAYIVNTARGKLCDRDAVARALESGRLAGYAGDVWFPQPAPKDHPWRTMPYNGMTPHISGTTLTAQARYAAGTREILECFFEGRPIRDEYLIVQGGALAGTGAHSYSKGNATGGSEEAAKFKKAV
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Predicted Molecular Mass
44.2 kDa
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Molecular Weight
Approximately 44 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 20 mM HEPES, pH 7.5, 100 mM NaCl.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (236 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)