1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. FDPS Protein, Mouse (His)

FDPS Protein, Mouse (His)

Cat. No.: HY-P75196
COA Handling Instructions

FDPS proteins are key enzymes in isoprenoid biosynthesis and play a crucial role in the formation of farnesyl diphosphate (FPP). FPP is a precursor of essential metabolites involved in protein farnesylation and geranylgeranylation. FDPS Protein, Mouse (His) is the recombinant mouse-derived FDPS protein, expressed by E. coli , with N-His labeled tag. The total length of FDPS Protein, Mouse (His) is 353 a.a., with molecular weight of ~42.8 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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  • Documentation

Description

FDPS proteins are key enzymes in isoprenoid biosynthesis and play a crucial role in the formation of farnesyl diphosphate (FPP). FPP is a precursor of essential metabolites involved in protein farnesylation and geranylgeranylation. FDPS Protein, Mouse (His) is the recombinant mouse-derived FDPS protein, expressed by E. coli , with N-His labeled tag. The total length of FDPS Protein, Mouse (His) is 353 a.a., with molecular weight of ~42.8 kDa.

Background

Farnesyl diphosphate synthase (FDPS) emerges as a pivotal enzyme in isoprenoid biosynthesis, driving the crucial formation of farnesyl diphosphate (FPP). FPP, a key precursor for various essential metabolites such as sterols, dolichols, carotenoids, and ubiquinones, also serves as a substrate for protein farnesylation and geranylgeranylation. The catalytic activity of FDPS involves the sequential condensation of isopentenyl pyrophosphate with the allylic pyrophosphates, first with dimethylallyl pyrophosphate and then with the resulting geranylpyrophosphate, ultimately yielding the essential product, farnesyl pyrophosphate. This intricate enzymatic process underscores FDPS's central role in the synthesis of fundamental cellular components and post-translational modifications critical for diverse cellular functions.

Biological Activity

Measured in a cell proliferation assay using H4 human neuroglioma cells. The ED50 for this effect is 2.560 ng/mL. Corresponding to a specific activity is 3.906×10[5] Unit/mg.

Species

Mouse

Source

E. coli

Tag

N-His

Accession

Q920E5 (M1-K353)

Gene ID

110196  [NCBI]

Molecular Construction
N-term
His
FDPS (M1-K353)
Accession # Q920E5
C-term
Synonyms
Farnesyl pyrophosphate synthase; FPP synthase; CR 39; Fdps
AA Sequence

MNGNQKLDAYNQEKQNFIQHFSQIVKVLTEKELGHPEIGDAIARLKEVLEYNALGGKYNRGLTVVQAFQELVEPKKQDAESLQRALTVGWCVELLQAFFLVSDDIMDSSLTRRGQICWYQKPGIGLDAINDALLLEASIYRLLKFYCREQPYYLNLLELFLQSSYQTEIGQTLDLMTAPQGHVDLGRYTEKRYKSIVKYKTAFYSFYLPIAAAMYMAGIDGEKEHANALKILMEMGEFFQVQDDYLDLFGDPSVTGKVGTDIQDNKCSWLVVQCLLRASPQQRQILEENYGQKDPEKVARVKALYEALDLQSAFFKYEEDSYNRLKSLIEQCSAPLPPSIFMELANKIYKRRK

Molecular Weight

Approximately 42.8 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

FDPS Protein, Mouse (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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FDPS Protein, Mouse (His)
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HY-P75196
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