FLG Protein, Human (His)
FLG Protein, essential in the mammalian epidermis, plays a key role in terminal differentiation. It aggregates keratin intermediate filaments and promotes disulfide bond formation, emphasizing its crucial contribution to the structural organization and integrity of the epidermis in the concluding stages of cellular differentiation. FLG, Human (His) is the recombinant human-derived FLG protein, expressed by E. coli, with N-6*His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
FLG Protein, essential in the mammalian epidermis, plays a key role in terminal differentiation. It aggregates keratin intermediate filaments and promotes disulfide bond formation, emphasizing its crucial contribution to the structural organization and integrity of the epidermis in the concluding stages of cellular differentiation. FLG, Human (His) is the recombinant human-derived FLG protein, expressed by E. coli, with N-6*His labeled tag.
Background
FLG, a crucial protein in the mammalian epidermis, plays a pivotal role in the terminal differentiation process by aggregating keratin intermediate filaments and facilitating the formation of disulfide bonds among these filaments. This function underscores the significance of FLG in the structural organization and integrity of the epidermis during the final stages of cellular differentiation.
Technical Parameters
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Species Human
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Source E. coli
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Tag C-6*His
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Accession
P20930 (M1-D300)
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Gene ID2312
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Molecular Construction
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N-term
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FLG (M1-D300)
Accession # P20930 -
6*His
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C-term
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Protein Length
Partial
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Synonyms
FLG; Truncated Profilaggrin; Filaggrin; Epidermal Filaggrin; FLG-1; Profilaggrin; FLG1; Protein S100; S100 Calcium-Binding Protein; ATOD2; Alternative Protein FLG
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AA Sequence
MSTLLENIFAIINLFKQYSKKDKNTDTLSKKELKELLEKEFRQILKNPDDPDMVDVFMDHLDIDHNKKIDFTEFLLMVFKLAQAYYESTRKENLPISGHKHRKHSHHDKHEDNKQEENKENRKRPSSLERRNNRKGNKGRSKSPRETGGKRHESSSEKKERKGYSPTHREEEYGKNHHNSSKKEKNKTENTRLGDNRKRLSERLEEKEDNEEGVYDYENTGRMTQKWIQSGHIATYYTIQDEAYDTTDSLLEENKIYERSRSSDGKSSSQVNRSRHENTSQVPLQESRTRKRRGSRVSQD
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Predicted Molecular Mass
42.5 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE .
Product Properties
Lyophilized powder
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O . For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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