FPG Protein, E.coli (His)
FPG is a key base excision repair enzyme that recognizes and eliminates damaged bases, especially oxidized purines such as 8-oxoG. It acts on a variety of pathologies, including guanidinohydantoin: C, spiroiminodiahydantoin: C, thymine glycol: G, 5,6-dihydrouracil: G, and 5-hydroxy Uracil: G. FPG Protein, E.coli (His) is the recombinant E. coli-derived FPG protein, expressed by E. coli , with His tag. FPG Protein, E.coli (His), has molecular weight of ~30.3 kDa.
- Species: E.coli
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
FPG is a key base excision repair enzyme that recognizes and eliminates damaged bases, especially oxidized purines such as 8-oxoG. It acts on a variety of pathologies, including guanidinohydantoin: C, spiroiminodiahydantoin: C, thymine glycol: G, 5,6-dihydrouracil: G, and 5-hydroxy Uracil: G. FPG Protein, E.coli (His) is the recombinant E. coli-derived FPG protein, expressed by E. coli , with His tag. FPG Protein, E.coli (His), has molecular weight of ~30.3 kDa.
Background
FPG, an essential player in base excision repair, addresses DNA damage resulting from oxidation or exposure to mutagenic agents. Functioning as a DNA glycosylase, FPG recognizes and eliminates damaged bases, exhibiting a preference for oxidized purines like 7,8-dihydro-8-oxoguanine (8-oxoG) and its derivatives, including guanidinohydantoin:C and spiroiminodihydantoin:C. Additionally, FPG acts on thymine glycol:G, 5,6-dihydrouracil:G, and 5-hydroxyuracil:G. Notably, FPG possesses AP (apurinic/apyrimidinic) lyase activity, introducing nicks in the DNA strand. Through beta-delta elimination, it cleaves the DNA backbone, generating a single-strand break at the site of the removed base, encompassing both 3'- and 5'-phosphates. Moreover, FPG efficiently cleaves single-stranded DNA containing an AP site, contributing to the repair of DNA lesions.
Technical Parameters
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Species E.coli
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Source E. coli
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Tag His
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Accession
P05523 Sequence information is not available or disclosed for this product.
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Gene ID946765 [NCBI]
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Synonyms
Formamidopyrimidine-DNA glycosylase; EC:3.2.2.23; Fapy-DNA glycosylase; DNA-(apurinic or apyrimidinic site) lyase MutM; AP lyase MutM; EC:4.2.99.18; mutM; fpg; b3635; JW3610
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AA Sequence
MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRLSDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKVLRYTDPRRFGAWLWTKELEGHNVLTHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMDNKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLKDFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK
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Molecular Weight
Approximately 30 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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