FPG Protein, E.coli (His)

FPG is a key base excision repair enzyme that recognizes and eliminates damaged bases, especially oxidized purines such as 8-oxoG. It acts on a variety of pathologies, including guanidinohydantoin: C, spiroiminodiahydantoin: C, thymine glycol: G, 5,6-dihydrouracil: G, and 5-hydroxy Uracil: G. FPG Protein, E.coli (His) is the recombinant E. coli-derived FPG protein, expressed by E. coli , with His, tag free. FPG Protein, E.coli (His), has molecular weight of ~30.3 kDa.

FPG, an essential player in base excision repair, addresses DNA damage resulting from oxidation or exposure to mutagenic agents. Functioning as a DNA glycosylase, FPG recognizes and eliminates damaged bases, exhibiting a preference for oxidized purines like 7,8-dihydro-8-oxoguanine (8-oxoG) and its derivatives, including guanidinohydantoin:C and spiroiminodihydantoin:C. Additionally, FPG acts on thymine glycol:G, 5,6-dihydrouracil:G, and 5-hydroxyuracil:G. Notably, FPG possesses AP (apurinic/apyrimidinic) lyase activity, introducing nicks in the DNA strand. Through beta-delta elimination, it cleaves the DNA backbone, generating a single-strand break at the site of the removed base, encompassing both 3'- and 5'-phosphates. Moreover, FPG efficiently cleaves single-stranded DNA containing an AP site, contributing to the repair of DNA lesions.

E.coli

E. coli

His

P05523

946765  [NCBI]

MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRLSDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKVLRYTDPRRFGAWLWTKELEGHNVLTHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMDNKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLKDFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK

Approximately 30.3 kDa

Greater than 95% as determined by reducing SDS-PAGE.