Glutathione synthetase/GSS Protein, Human (His)

Glutathione synthase/GSS protein catalyzes the ATP-dependent synthesis of glutathione from gamma-glutamylcysteine and glycine. Glutathione synthetase/GSS Protein, Human (His) is the recombinant human-derived Glutathione synthetase/GSS protein, expressed by E. coli , with C-6*His labeled tag. The total length of Glutathione synthetase/GSS Protein, Human (His) is 473 a.a., with molecular weight of ~55.0 kDa.

The Glutathione synthetase (GSS) protein plays a pivotal role in cellular antioxidant defense by catalyzing the synthesis of glutathione from gamma-glutamylcysteine and glycine in an ATP-dependent manner, as supported by various studies. Glutathione (GSH), composed of gamma-glutamylcysteinylglycine, stands as the most abundant intracellular thiol in aerobic cells, and its synthesis by GSS is essential for diverse cellular processes. These include safeguarding cells against oxidative damage, facilitating amino acid transport, detoxifying foreign compounds, maintaining protein sulfhydryl groups in a reduced state, and acting as a cofactor for numerous enzymes. Moreover, GSS's involvement in ophthalmate biosynthesis in hepatocytes underscores its versatile role in cellular metabolism and redox homeostasis.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

C-6*His

P48637 (A2-V474)

2937  [NCBI]

ATNWGSLLQDKQQLEELARQAVDRALAEGVLLRTSQEPTSSEVVSYAPFTLFPSLVPSALLEQAYAVQMDFNLLVDAVSQNAAFLEQTLSSTIKQDDFTARLFDIHKQVLKEGIAQTVFLGLNRSDYMFQRSADGSPALKQIEINTISASFGGLASRTPAVHRHVLSVLSKTKEAGKILSNNPSKGLALGIAKAWELYGSPNALVLLIAQEKERNIFDQRAIENELLARNIHVIRRTFEDISEKGSLDQDRRLFVDGQEIAVVYFRDGYMPRQYSLQNWEARLLLERSHAAKCPDIATQLAGTKKVQQELSRPGMLEMLLPGQPEAVARLRATFAGLYSLDVGEEGDQAIAEALAAPSRFVLKPQREGGGNNLYGEEMVQALKQLKDSEERASYILMEKIEPEPFENCLLRPGSPARVVQCISELGIFGVYVRQEKTLVMNKHVGHLLRTKAIEHADGGVAAGVAVLDNPYPV

Approximately 55.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 200 mM NaCl, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.