glycoprotein B/gB Protein, HHV-7 (Cell-Free, His)
Glycoprotein B (gB) is an important envelope glycoprotein that forms spikes on the viral envelope and facilitates initial attachment to the heparan sulfate moiety on the host cell surface. gB is essential for viral entry and is involved in mediating fusion between the viral and cell membranes. glycoprotein B/gB Protein, HHV-7 (Cell-Free, His) is the recombinant Virus-derived glycoprotein B/gB protein, expressed by E. coli Cell-free , with N-10*His labeled tag.
- Species: Virus
- Source: E. coli Cell-free
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Glycoprotein B (gB) is an important envelope glycoprotein that forms spikes on the viral envelope and facilitates initial attachment to the heparan sulfate moiety on the host cell surface. gB is essential for viral entry and is involved in mediating fusion between the viral and cell membranes. glycoprotein B/gB Protein, HHV-7 (Cell-Free, His) is the recombinant Virus-derived glycoprotein B/gB protein, expressed by E. coli Cell-free , with N-10*His labeled tag.
Background
The glycoprotein B (gB) protein is an envelope glycoprotein that forms spikes on the surface of the viral envelope. It is essential for the initial attachment to heparan sulfate moieties found on the host cell surface proteoglycans. Furthermore, gB is involved in the fusion of viral and cellular membranes, facilitating the entry of the virus into the host cell. This fusion process is mediated by a fusion machinery consisting of at least gB and the heterodimer gH/gL. Additionally, gB may play a role in the fusion between the virion envelope and the outer nuclear membrane during virion egress. It exists as a homotrimer connected by disulfide bonds and is capable of binding to heparan sulfate proteoglycans. Moreover, gB interacts with the gH/gL heterodimer.
Technical Parameters
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Species Virus
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Source E. coli Cell-free
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Tag N-10*His
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Accession
P52352 (D23-L822)
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Gene ID/
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Molecular Construction
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N-term
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10*His
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HHV-7 gB (D23-L822)
Accession # P52352 -
C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
Envelope glycoprotein B; gB; UL27
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AA Sequence
DFVMTGHNQHLPFRICSIATGTDLVRFDREVSCASYGSNIKTTEGILIIYKTKIEAHTFSVRTFKKELTFQTTYRDVGTVYFLDRTVTTLPMPIEEVHMVNTEARCLSSISVKRSEEEEYVAYHKDEYVNKTLDLIPLNFKSDTVRRYITTKEPFLRNGPLWFYSTSTSINCIVTDCIAKTKYPFDFFALSTGETVEGSPFYNGINSKTFNEPTEKILFRNNYTMLKTFDDGSKGNFVTLTKMAFLEKGNTIFSWEVQNEESSICLLKHWMTIPHALRAENANSFHFIAQELTASFVTGKSNYTLSDSKYNCINSNYTSILDEIYQTQYNNSHDKNGSYEIFKTEGDLILIWQPLIQRKLTVLENFSNASRKRRKRELETNKDIVYVQLQYLYDTLKDYINTALGKLAEAWCLNQKRTITVLHELSKISPSGIISAVYGKPMSAKLIGDVLAVSKCIEVNQTSVQLHKSMRLTKDSSYDALRCYSRPLLTYSFANSSKETYLGQLGLDNEILLGNHRTEECEQSNTKIFLSGKFAHIFKDYTYVNSSLITEIEALDAFVDLNIDPLENADFTLLELYTKDELSKANVFDLETILREYNSYKSALHHIETKIATVTPTYIGGIDTFFKGLGALGLGLGAVLGVTAGALGDVVNGVFSFLKNPFGGALTILLTLGVIGLVIFLFLRHKRLAQTPIDILFPYTSKSTNSVLQATQSVQAQVKEPLDSSPPYLKTNKDTEPQGDDITHTNEYSQVEALKMLKAIKLLDESYKKAEIAEAKKSQRPSLLERIQYRGYQKLSTEEL
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Predicted Molecular Mass
93.5 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add 5-50% of glycerol (final concentration). Our default final concentration of glycerol is 50%. Customers could use it as reference.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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