gyaR Protein, Thermoanaerobacter ethanolicus (His, StrepⅡ)
GyaR Proteinas, an alcohol dehydrogenase, efficiently oxidizes primary long-chain alcohols, with pentan-1-ol as the optimal substrate in vitro. It displays versatile dehydrogenase activity on alcohols like propanol, hexanol, and ethanol, highlighting its potential in diverse metabolic pathways by catalyzing the oxidation of various primary alcohols. gyaR Protein, Thermoanaerobacter ethanolicus (His, Strep) is the recombinant gyaR protein, expressed by E. coli , with N-Strep, N-6*His labeled tag.
- Species: Others
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Biological Activity
Description
GyaR Proteinas, an alcohol dehydrogenase, efficiently oxidizes primary long-chain alcohols, with pentan-1-ol as the optimal substrate in vitro. It displays versatile dehydrogenase activity on alcohols like propanol, hexanol, and ethanol, highlighting its potential in diverse metabolic pathways by catalyzing the oxidation of various primary alcohols. gyaR Protein, Thermoanaerobacter ethanolicus (His, Strep) is the recombinant gyaR protein, expressed by E. coli , with N-Strep, N-6*His labeled tag.
Background
GyaR, identified as an alcohol dehydrogenase, exhibits enzymatic activity against primary long-chain alcohols. While pentan-1-ol serves as the optimal substrate in vitro, GyaR displays efficient dehydrogenase activity on a range of alcohols, including propanol, hexanol, and ethanol. This versatility underscores the enzyme's capability to catalyze the oxidation of various primary alcohols, emphasizing its potential role in diverse metabolic pathways.
Technical Parameters
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Species Others
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Source E. coli
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Tag N-StrepⅡ;N-6*His
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Accession
Q9F282 (W2-F399)
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Gene ID/
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Molecular Construction
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N-term
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StrepⅡ-6*His
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gyaR (W2-F399)
Accession # Q9F282 -
C-term
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Protein Length
Full Length
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Synonyms
Long-chain primary alcohol dehydrogenase AdhA; EC:1.1.1.3; adhA
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AA Sequence
WETKINPNKVFELRCKNTTYFGIGSIKKIKDILEVLKNKGINNVILVTGKGSYKASGAWDVVKPALETLGFKYSLYDKVGPNPTVDMIDEAAKIGRETGAKAVIGIGGGSPIDTAKSVAVLLEYTDKNARELYEQKFIPEKAAPIIAINLTHGTGTEVDRFAVATIPEKNYKPAIAYDCLYPMYAIDDPSLMTKLDKKQTIAVTIDALNHVTEAATTLVASPYSVLMAKETVRLIVRYLPAAVNDPENLVARYYLLYASALAGISFDNGLLHLTHALEHPLSAVKPEIAHGLGLGAILPAVVKAIYPSVAEVLAEVYSPIVPGLKGLPAEAEYVAKKVEEWLFKVGCTQKLSDFGFTKEDIPTLVRLAKTTPSLDGLLSNAPVEATEAVIAKIYEESF
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)