Heat Stable FGF-basic Protein, Human

FGF basic, or bFGF (fibroblast growth factor basic), initiates at an alternative CUG codon, marking a distinctive feature in translational initiation. This alternative start codon plays a pivotal role in regulating the expression and functional properties of FGF basic. Heat Stable FGF-basic Protein, Human is the recombinant human-derived Heat Stable FGF-basic protein, expressed by E. coli , with tag free. The total length of Heat Stable FGF-basic Protein, Human is 154 a.a., with molecular weight of ~17.1 kDa.

Initiation of FGF basic, also known as bFGF (fibroblast growth factor basic), occurs at an alternative CUG codon. This alternative start codon represents a distinctive feature in the translational initiation of the protein, contributing to the unique regulatory mechanisms governing the expression and functional properties of FGF basic.

Measure by its ability by a dose-response proliferation assay using human 3T3 cells. The ED₅₀ for this effect is <0.25 ng/mL. The specific activity of this protein is > 4.0 × 10⁶ IU/mg. (It is recommended to experimentally determine the optimal concentration for each specific application by performing a dose response assay.)

Human

E. coli

Tag Free

P09038-4 (A135-S288) with an N-terminal M and several site mutations

2247

/

Approximately 17.1 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

< 0.2 EU/μg of protein by gel clotting method

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.