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  5. HER2/CD340 Protein, Rhesus Macaque (HEK293, His)

HER2/CD340 Protein, Rhesus Macaque (HEK293, His)

Cat. No.: HY-P75807
COA Handling Instructions

The HER2/CD340 protein is a multifunctional tyrosine kinase that is a component of the neuregulin receptor complex and regulates microtubule dynamics. Activated HER2/CD340 triggers the MEMO1-RHOA-DIAPH1 pathway, inhibits GSK3B on the cell membrane and promotes the binding of APC and CLASP2, which is critical for microtubule stability. HER2/CD340 Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived HER2/CD340 protein, expressed by HEK293 , with C-His labeled tag. The total length of HER2/CD340 Protein, Rhesus Macaque (HEK293, His) is 629 a.a., with molecular weight of ~90-110 kDa.

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Description

The HER2/CD340 protein is a multifunctional tyrosine kinase that is a component of the neuregulin receptor complex and regulates microtubule dynamics. Activated HER2/CD340 triggers the MEMO1-RHOA-DIAPH1 pathway, inhibits GSK3B on the cell membrane and promotes the binding of APC and CLASP2, which is critical for microtubule stability. HER2/CD340 Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived HER2/CD340 protein, expressed by HEK293 , with C-His labeled tag. The total length of HER2/CD340 Protein, Rhesus Macaque (HEK293, His) is 629 a.a., with molecular weight of ~90-110 kDa.

Background

HER2/CD340 protein, a multifaceted protein tyrosine kinase, serves as an essential component within various cell surface receptor complexes, requiring a coreceptor for ligand binding. Integral to neuregulin-receptor complexes, it collaborates with neuregulins, and GP30 emerges as a potential ligand for this receptor. Beyond its receptor roles, HER2/CD340 plays a pivotal role in regulating the outgrowth and stabilization of peripheral microtubules (MTs). Upon activation, the MEMO1-RHOA-DIAPH1 signaling pathway, triggered by ERBB2 activation, leads to GSK3B phosphorylation and subsequent inhibition at the cell membrane. This orchestrated inhibition prevents the phosphorylation of APC and CLASP2, facilitating their association with the cell membrane. The membrane-bound APC, in turn, facilitates the localization of MACF1 to the cell membrane, a critical step for microtubule capture and stabilization. Inside the nucleus, HER2/CD340 is involved in transcriptional regulation, associating with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activating its transcription. Additionally, it participates in the transcription of rRNA genes by RNA Pol I, enhancing protein synthesis and promoting cell growth. The multifaceted functions of HER2/CD340 underscore its central role in diverse cellular processes, from receptor signaling to microtubule dynamics and transcriptional regulation.

Biological Activity

Measured by its binding ability in a functional ELISA Immobilized Rhesus Macaque HER2 at 2 μg/mL (100 μL/well) can bind Anti-Erbb2 antibody, The ED50 for this effect is 14.33 ng/mL.

Species

Rhesus Macaque

Source

HEK293

Tag

C-His

Accession

XP_001090430 (Q24-T652)

Gene ID
Molecular Construction
N-term
HER2 (Q24-T652)
Accession # XP_001090430
His
C-term
Synonyms
Receptor tyrosine-protein kinase erbB-2; p185neu; CD340; Erbb2; Neu
AA Sequence

QVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDLLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPVCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAGCKKIFGSLAFLPESFDGDPASNTAPLQPEQLRVFETLEEITGYLYISAWPDSLPDLSVLQNLQVIRGRILHNGAYSLTLQGLGISWLGLRSLRELGSGLALIHHNTRLCFVHTVPWDQLFRNPHQALLHTANRPEDECVGEGLACHQLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGPEADQCVACAHYKDPPFCVARCPSGVKPDLSYMPIWKFPDEEGTCQSCPINCTHSCVDLDDKGCPAEQRASPLT

Molecular Weight

Approximately 90-110 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HER2/CD340 Protein, Rhesus Macaque (HEK293, His)
Cat. No.:
HY-P75807
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