HER2/CD340 Protein, Rhesus Macaque (HEK293, His)

The HER2/CD340 protein is a multifunctional tyrosine kinase that is a component of the neuregulin receptor complex and regulates microtubule dynamics. Activated HER2/CD340 triggers the MEMO1-RHOA-DIAPH1 pathway, inhibits GSK3B on the cell membrane and promotes the binding of APC and CLASP2, which is critical for microtubule stability. HER2/CD340 Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived HER2/CD340 protein, expressed by HEK293 , with C-His labeled tag.

HER2/CD340 protein, a multifaceted protein tyrosine kinase, serves as an essential component within various cell surface receptor complexes, requiring a coreceptor for ligand binding. Integral to neuregulin-receptor complexes, it collaborates with neuregulins, and GP30 emerges as a potential ligand for this receptor. Beyond its receptor roles, HER2/CD340 plays a pivotal role in regulating the outgrowth and stabilization of peripheral microtubules (MTs). Upon activation, the MEMO1-RHOA-DIAPH1 signaling pathway, triggered by ERBB2 activation, leads to GSK3B phosphorylation and subsequent inhibition at the cell membrane. This orchestrated inhibition prevents the phosphorylation of APC and CLASP2, facilitating their association with the cell membrane. The membrane-bound APC, in turn, facilitates the localization of MACF1 to the cell membrane, a critical step for microtubule capture and stabilization. Inside the nucleus, HER2/CD340 is involved in transcriptional regulation, associating with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activating its transcription. Additionally, it participates in the transcription of rRNA genes by RNA Pol I, enhancing protein synthesis and promoting cell growth. The multifaceted functions of HER2/CD340 underscore its central role in diverse cellular processes, from receptor signaling to microtubule dynamics and transcriptional regulation.

1.This product does not contain protein kinase domain.
2.Measured by its binding ability in a functional ELISA Immobilized Rhesus Macaque HER2 at 2 μg/mL (100 μL/well) can bind Anti-Erbb2 antibody, The ED₅₀ for this effect is 14.33 ng/mL.

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  Measured by its binding ability in a functional ELISA Immobilized Rhesus Macaque HER2 at 2 μg/mL (100 μL/well) can bind Anti-Erbb2 antibody, The ED₅₀ for this effect is 14.33 ng/mL.

Rhesus Macaque

HEK293

C-His

XP_001090430.1 (Q24-T652)

697573  [NCBI]

Partial

QVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDLLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPVCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAGCKKIFGSLAFLPESFDGDPASNTAPLQPEQLRVFETLEEITGYLYISAWPDSLPDLSVLQNLQVIRGRILHNGAYSLTLQGLGISWLGLRSLRELGSGLALIHHNTRLCFVHTVPWDQLFRNPHQALLHTANRPEDECVGEGLACHQLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGPEADQCVACAHKDPPFCVARCPSGVKPDLSYMPIWKFPDEEGTCQSCPINCTHSCVDLDDKGCPAEQRASPLT

Approximately 90-110 kDa

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. /biology-dictionary/nci-h1395-cell.html