HER3 Protein, Mouse (HEK293, His)
Based on 1 Customer Validation
HER3 Protein, a vital tyrosine-protein kinase and neuregulin cell surface receptor, activates upon binding to neuregulin-1 (NRG1), enhancing phosphorylation on tyrosine residues and associating with the p85 subunit of phosphatidylinositol 3-kinase. It is also activated by CSPG5 and plays a crucial role in regulating myeloid cell differentiation. HER3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived HER3 protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Mouse
- Source: HEK293
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
HER3 Protein, a vital tyrosine-protein kinase and neuregulin cell surface receptor, activates upon binding to neuregulin-1 (NRG1), enhancing phosphorylation on tyrosine residues and associating with the p85 subunit of phosphatidylinositol 3-kinase. It is also activated by CSPG5 and plays a crucial role in regulating myeloid cell differentiation. HER3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived HER3 protein, expressed by HEK293 , with C-6*His labeled tag.
Background
HER3 protein is a crucial tyrosine-protein kinase functioning as a cell surface receptor for neuregulins. Upon binding neuregulin-1 (NRG1), it undergoes activation, leading to increased phosphorylation on tyrosine residues and subsequent association with the p85 subunit of phosphatidylinositol 3-kinase. Additionally, HER3 protein can be activated by CSPG5. Notably, it plays a significant role in regulating myeloid cell differentiation.
Verified Bioactivity
1.This product does not contain protein kinase domain.
2.Immobilized Mouse Her3, His Tag at 2 μg/mL (100 μL/well) on the plate. Dose response curve for Human NRG1 Beta 1, hFc Tag with the EC50 of 30-80 ng/mL determined by ELISA.
Technical Parameters
-
Species Mouse
-
Source HEK293
-
Tag C-6*His
-
Accession
Q61526 (S20-H641)
-
Molecular Construction
-
N-term
-
HER3 (S20-H641)
Accession # Q61526 -
6*His
-
C-term
-
-
Protein Length
Extracellular Domain
-
Synonyms
ERBB3; Receptor Protein-Tyrosine Kinase; Prev. LCCS2; P45-SErbB3; HER3; P85-SErbB3; V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncogene Homolog 3; ErbB-3 R31; Tyrosine Kinase-Type Cell Surface Receptor HER3; MDA-BF-1; Human Epidermal Growth Factor Receptor 3; C-ErbB3; Receptor Tyrosine-Protein Kinase ErbB-3; ErbB3-S; Proto-Oncogene-Like Protein C-ErbB-3; ErbB-3; C-ErbB-3; FERLK; CDNA FLJ43038 Fis, Clone BRTHA3002955, Highly Similar To Receptor Tyrosine-Protein Kinase ErbB-3; VSCN1; Lethal Congenital Contracture Syndrome 2; Erb-B2 Receptor Tyrosine Kinase 3; P180-ErbB3
-
AA Sequence
SEMGNSQAVCPGTLNGLSVTGDADNQYQTLYKLYEKCEVVMGNLEIVLTGHNADLSFLQWIREVTGYVLVAMNEFSVLPLPNLRVVRGTQVYDGKFAIFVMLNYNTNSSHALRQLRFTQLTEILLGGVYIEKNDKLCHMDTIDWRDIVRVPDAEIVVKNNGGNCPPCHEVCKGRCWGPGPEDCQILTKTICAPQCNGRCFGPNPNQCCHDECAGGCSGPQDTDCFACRHFNDSGACVPRCPAPLVYNKLTFQLEPNPHIKYQYGGVCVASCPHNFVVDQTFCVRACPADKMEVDKNGLKMCEPCRGLCPKACEGTGSGSRYQTVDSSNIDGFVNCTKILGNLDFLITGLNGDPWHKIPALDPEKLNVFRTVREITGYLNIQSWPPHMHNFSVFSNLTTIGGRSLYNRGFSLLIMKNLNVTSLGFRSLKEISAGRVYISANQQLCYHHSLNWTRLLRGPAEERLDIKYNRPLGECVAEGKVCDPLCSSGGCWGPGPGQCLSCRNYSREGVCVTHCNVLQGEPREFVHEAHCFSCHPECQPMEGTSTCNGSGSDACARCAHFRDGPHCVNSCPHGILGAKGPIYKYPDAQNECRPCHENCTQGCKGPELQDCLGQAEVLMSKPH
-
Predicted Molecular Mass
69.3 kDa
-
Molecular Weight
Approximately 80-120 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
-
Glycosylation
Yes
-
Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 8% trehalose.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
