HEXB/Hexosaminidase B Protein, Human (HEK293, His)

HEXB (hexosaminidase B) proteolytically hydrolyzes N-acetyl-D-hexosamine and/or sulfated residues in glycoconjugates, including oligosaccharides, glycolipids, and mucopolysaccharides. B isoenzymes efficiently hydrolyze neutral oligosaccharides, making them unique. HEXB/Hexosaminidase B Protein, Human (HEK293, His) is the recombinant human-derived HEXB/Hexosaminidase B protein, expressed by HEK293 , with C-His labeled tag.

HEXB (Hexosaminidase B) protein exhibits hydrolytic activity towards the non-reducing end N-acetyl-D-hexosamine and/or sulfated N-acetyl-D-hexosamine residues found in various glycoconjugates, including oligosaccharide moieties from proteins, neutral glycolipids, and specific mucopolysaccharides. Notably, the B isozyme demonstrates efficient hydrolysis of neutral oligosaccharides, distinguishing it from other substrates. While isozyme A is responsible for the degradation of GM2 gangliosides in the presence of GM2A, isozyme B does not participate in this process. During fertilization, HEXB, specifically isozyme B, plays a crucial role in the zona block to polyspermy. It is localized in the cortical granules of non-activated oocytes and is exocytosed during the cortical reaction triggered by oocyte activation. This exocytosis results in the inactivation of the sperm galactosyltransferase-binding site, contributing to the prevention of sperm binding to the zona pellucida and ensuring the integrity of the zona block to polyspermy.

Measured by its ability to hydrolyze 4-Methylumbelliferyl-N-acetyl-β-D-glucosaminide (4-MU-GlcNAC) and the specific activity is > 3000 pmols/min/μg.

Human

HEK293

C-His

P07686 (A43-M556)

3074  [NCBI]

Full Length of Mature Protein

ARAPSVSAKPGPALWPLPLLVKMTPNLLHLAPENFYISHSPNSTAGPSCTLLEEAFRRYHGYIFGFYKWHHEPAEFQAKTQVQQLLVSITLQSECDAFPNISSDESYTLLVKEPVAVLKANRVWGALRGLETFSQLVYQDSYGTFTINESTIIDSPRFSHRGILIDTSRHYLPVKIILKTLDAMAFNKFNVLHWHIVDDQSFPYQSITFPELSNKGSYSLSHVYTPNDVRMVIEYARLRGIRVLPEFDTPGHTLSWGKGQKDLLTPCYSRQNKLDSFGPINPTLNTTYSFLTTFFKEISEVFPDQFIHLGGDEVEFKCWESNPKIQDFMRQKGFGTDFKKLESFYIQKVLDIIATINKGSIVWQEVFDDKAKLAPGTIVEVWKDSAYPEELSRVTASGFPVILSAPWYLDLISYGQDWRKYYKVEPLDFGGTQKQKQLFIGGEACLWGEYVDATNLTPRLWPRASAVGERLWSSKDVRDMDDAYDRLTRHRCRMVERGIAAQPLYAGYCNHENM

Approximately 60-66 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.