HEXB/Hexosaminidase B Protein, Human (HEK293, His)

HEXB (hexosaminidase B) proteolytically hydrolyzes N-acetyl-D-hexosamine and/or sulfated residues in glycoconjugates, including oligosaccharides, glycolipids, and mucopolysaccharides. B isoenzymes efficiently hydrolyze neutral oligosaccharides, making them unique. HEXB/Hexosaminidase B Protein, Human (HEK293, His) is the recombinant human-derived HEXB/Hexosaminidase B protein, expressed by HEK293 , with C-His labeled tag. The total length of HEXB/Hexosaminidase B Protein, Human (HEK293, His) is 514 a.a., with molecular weight of 60-66 kDa.

HEXB (Hexosaminidase B) protein exhibits hydrolytic activity towards the non-reducing end N-acetyl-D-hexosamine and/or sulfated N-acetyl-D-hexosamine residues found in various glycoconjugates, including oligosaccharide moieties from proteins, neutral glycolipids, and specific mucopolysaccharides. Notably, the B isozyme demonstrates efficient hydrolysis of neutral oligosaccharides, distinguishing it from other substrates. While isozyme A is responsible for the degradation of GM2 gangliosides in the presence of GM2A, isozyme B does not participate in this process. During fertilization, HEXB, specifically isozyme B, plays a crucial role in the zona block to polyspermy. It is localized in the cortical granules of non-activated oocytes and is exocytosed during the cortical reaction triggered by oocyte activation. This exocytosis results in the inactivation of the sperm galactosyltransferase-binding site, contributing to the prevention of sperm binding to the zona pellucida and ensuring the integrity of the zona block to polyspermy.

Measured by its ability to hydrolyze 4methylumbelliferylNacetylβDglucosaminide (4MUGlcNAc) and the specific activity is > 3000 pmols/min/μg.

Human

HEK293

C-His

P07686 (A43-M556)

3074  [NCBI]

60-66 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.