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HGF Protein, Human (HEK293, His)

2 Cited Publications
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Based on 2 publication(s) in Google Scholar

HGF Protein is a multifunctional glycoprotein. By binding to the transmembrane tyrosine kinase receptor Met, HGF Protein plays a key role in cell proliferation, migration, differentiation and tissue repair. Abnormalities of HGF Protein are closely associated with tumor growth, metastasis and organ fibrosis. HGF Protein, Human (HEK293, His) is a recombinant HGF protein expressed by HEK293 and tagged with C-His.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for HGF Protein, Human (HEK293, His)
  • Experimental Validation Results for HGF Protein, Human (HEK293, His)
  • Experimental Validation Results for HGF Protein, Human (HEK293, His)
  • HGF Protein, Human (HEK293, His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
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Biological Activity

Description

HGF Protein is a multifunctional glycoprotein. By binding to the transmembrane tyrosine kinase receptor Met, HGF Protein plays a key role in cell proliferation, migration, differentiation and tissue repair. Abnormalities of HGF Protein are closely associated with tumor growth, metastasis and organ fibrosis. HGF Protein, Human (HEK293, His) is a recombinant HGF protein expressed by HEK293 and tagged with C-His[1][2][3].

Background

HGF is a multifunctional cytokine mainly secreted by mesenchymal cells. HGF activates downstream signaling pathways by binding to the transmembrane tyrosine kinase receptor Met. HGF is indispensable for embryonic development, and knockout of related genes leads to embryonic lethality. HGF has multiple biological functions, including inducing cell proliferation, migration, morphogenesis, and angiogenesis. HGF is involved in the regeneration and repair of multiple organs such as the liver, kidney, and lung, and can improve organ damage through anti-apoptotic, anti-fibrotic, and anti-inflammatory effects. Meanwhile, abnormal activation of the HGF/Met signaling pathway is closely associated with tumor invasion and metastasis. HGF has dual functions: it can promote tissue repair and inhibit tumor progression through antagonistic mechanisms[1][2][3].

In Vitro

HGF Protein (Human; 0-10 ng/mL; 72 h) promotes DNA synthesis in primary rat and human hepatocytes[4].

In Vivo

HGF Protein (Human; 500 μg; intra-arterial injection; 2 times or 3 mg; intravenous injection; 5 times) induces angiogenesis in a rabbit hindlimb ischemia model, significantly promoting collateral vessel formation and improving angiographic scores[5].

Verified Bioactivity

1.The ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells has an ED50 value of <2 ng/mL.
2.Immobilized Human HGF, His Tag at 1 μg/mL (100 μl/Well) on the plate. Dose response curve for Human HGF R, hFc Tag with the EC50 of ≤28.2 ng/mL determined by ELISA.
3. Measured by its binding ability in a functional ELISA. Immobilized Human HGF at 2 μg/mL (100 μL/well) can bind Human HGFR. The ED50 for this effect is 14.09-20 ng/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized Human HGF at 2 μg/mL (100 μL/well) can bind Human HGFR. The ED50 for this effect is 19.58 ng/mL.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells. The ED50 for this effect is 1.6 ng/mL, corresponding to a specific activity is 6.25×105 U/mg.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    P14210-1 (Q32-S728)

  • Gene ID
    3082  [NCBI]
  • Molecular Construction
    • N-term
    • HGF (Q32-S728)
      Accession # P14210
    • His
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    HGF; Hepatocyte Growth Factor (Hepapoietin A; Scatter Factor); Prev. DFNB39; Lung Fibroblast-Derived Mitogen; HPTA; Hepatopoietin-A; SF; Scatter Factor; F-TCF; Deafness, Autosomal Recessive 39; HGFB; Hepatopoietin A; Hepatocyte Growth Factor; Fibroblast-Derived Tumor Cytotoxic Factor

  • AA Sequence

    QRKRRNTIHEFKKSAKTTLIKIDPALKIKTKKVNTADQCANRCTRNKGLPFTCKAFVFDKARKQCLWFPFNSMSSGVKKEFGHEFDLYENKDYIRNCIIGKGRSYKGTVSITKSGIKCQPWSSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGLMDHTESGKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGQPRPWCYTLDPHTRWEYCAIKTCADNTMNDTDVPLETTECIQGQGEGYRGTVNTIWNGIPCQRWDSQYPHEHDMTPENFKCKDLRENYCRNPDGSESPWCFTTDPNIRVGYCSQIPNCDMSHGQDCYRGNGKNYMGNLSQTRSGLTCSMWDKNMEDLHRHIFWEPDASKLNENYCRNPDDDAHGPWCYTGNPLIPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTRTNIGWMVSLRYRNKHICGGSLIKESWVLTARQCFPSRDLKDYEAWLGIHDVHGRGDEKCKQVLNVSQLVYGPEGSDLVLMKLARPAVLDDFVSTIDLPNYGCTIPEKTSCSVYGWGYTGLINYDGLLRVAHLYIMGNEKCSQHHRGKVTLNESEICAGAEKIGSGPCEGDYGGPLVCEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKIILTYKVPQS

  • Predicted Molecular Mass

    80.5 kDa

  • Molecular Weight

    Approximately 30-40 kDa (β subunit) & 50-65 kDa (α subunit) & 80-100 kDa (α subunit+β subunit),based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Structure/Form

    disulfide-linked heterodimer

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 500 mM NaCl, pH 8.0.
2.Lyophilized from a 0.22 μm filtered solution of PBS, 200mM L-arginine, pH 7.4.
3.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol, 0.01% Tween 80.
4.Lyophilized from a 0.22 μm filtered solution of PBS, pH 6.5, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Cecchi F, et al. Targeting the HGF/Met signalling pathway in cancer. Eur J Cancer. 2010 May;46(7):1260-70. [Content Brief]

[2]. Stella MC, et al. HGF: a multifunctional growth factor controlling cell scattering. Int J Biochem Cell Biol. 1999 Dec;31(12):1357-62. [Content Brief]

[3]. Nakamura T, et al. The discovery of hepatocyte growth factor (HGF) and its significance for cell biology, life sciences and clinical medicine. Proc Jpn Acad Ser B Phys Biol Sci. 2010;86(6):588-610. [Content Brief]

[4]. Strain AJ, et al. Native and recombinant human hepatocyte growth factors are highly potent promoters of DNA synthesis in both human and rat hepatocytes. J Clin Invest. 1991 May;87(5):1853-7. [Content Brief]

[5]. Morishita R, et al. Therapeutic angiogenesis induced by human recombinant hepatocyte growth factor in rabbit hind limb ischemia model as cytokine supplement therapy. Hypertension. 1999 Jun;33(6):1379-84. [Content Brief]

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Calculators

Reconstitution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized Human HGF at 2 μg/mL (100 μL/well) can bind Human HGFR. The ED50 for this effect is 19.58 ng/mL.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells. The ED50 for this effect is 1.6 ng/mL, corresponding to a specific activity is 6.25×105 U/mg.

  • HGF Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for HGF Protein, Human (HEK293, His)
  • Experimental Validation Results for HGF Protein, Human (HEK293, His)
  • Experimental Validation Results for HGF Protein, Human (HEK293, His)
  • HGF Protein, Human (HEK293, His) Product Image