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HGF Protein, Mouse (HEK293, His)

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HGF Protein, a potent mitogen, stimulates mature hepatocyte cell growth and acts as a hepatotrophic factor for various tissues. As an activating ligand for MET receptor tyrosine kinase, HGF binds and promotes dimerization, activating MAPK signaling through TMPRSS13 cleavage. Structurally, HGF comprises alpha and beta chains connected by a disulfide bond. Interaction with SRPX2 enhances its mitogenic activity. HGF Protein, Mouse (HEK293, His) is the recombinant mouse-derived HGF protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for HGF Protein, Mouse (HEK293, His)
  • Experimental Validation Results for HGF Protein, Mouse (HEK293, His)
  • HGF Protein, Mouse (HEK293, His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

HGF Protein, a potent mitogen, stimulates mature hepatocyte cell growth and acts as a hepatotrophic factor for various tissues. As an activating ligand for MET receptor tyrosine kinase, HGF binds and promotes dimerization, activating MAPK signaling through TMPRSS13 cleavage. Structurally, HGF comprises alpha and beta chains connected by a disulfide bond. Interaction with SRPX2 enhances its mitogenic activity. HGF Protein, Mouse (HEK293, His) is the recombinant mouse-derived HGF protein, expressed by HEK293 , with C-6*His labeled tag.

Background

HGF protein is a potent mitogen that stimulates the growth of mature parenchymal hepatocyte cells and acts as a hepatotrophic factor for a wide range of tissues and cell types. It functions as an activating ligand for the receptor tyrosine kinase MET, binding to it and facilitating its dimerization. HGF also activates MAPK signaling through the cleavage and activation of TMPRSS13. Structurally, HGF is composed of an alpha chain and a beta chain joined by a disulfide bond. Additionally, it interacts with SRPX2, promoting increased mitogenic activity of HGF.

Verified Bioactivity

Measured in a cell proliferation assay using mIMCD-3 mouse epithelial cells. The ED50 this effect is 5.0-15.0 ng/mL.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q08048-1 (Q33-L728)

  • Gene ID
    15234  [NCBI]
  • Molecular Construction
    • N-term
    • HGF (Q33-L728)
      Accession # Q848-1
    • His
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    HGF; Hepatocyte Growth Factor (Hepapoietin A; Scatter Factor); Prev. DFNB39; Lung Fibroblast-Derived Mitogen; HPTA; Hepatopoietin-A; SF; Scatter Factor; F-TCF; Deafness, Autosomal Recessive 39; HGFB; Hepatopoietin A; Hepatocyte Growth Factor; Fibroblast-D

  • AA Sequence

    QKKRRNTLHEFKKSAKTTLTKEDPLLKIKTKKVNSADECANRCIRNRGFTFTCKAFVFDKSRKRCYWYPFNSMSSGVKKGFGHEFDLYENKDYIRNCIIGKGGSYKGTVSITKSGIKCQPWNSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGPMDHTESGKTCQRWDQQTPHRHKFLPERYPDKGFDDNYCRNPDGKPRPWCYTLDPDTPWEYCAIKTCAHSAVNETDVPMETTECIQGQGEGYRGTSNTIWNGIPCQRWDSQYPHKHDITPENFKCKDLRENYCRNPDGAESPWCFTTDPNIRVGYCSQIPKCDVSSGQDCYRGNGKNYMGNLSKTRSGLTCSMWDKNMEDLHRHIFWEPDASKLNKNYCRNPDDDAHGPWCYTGNPLIPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTQTTVGWMVSLKYRNKHICGGSLIKESWVLTARQCFPARNKDLKDYEAWLGIHDVHERGEEKRKQILNISQLVYGPEGSDLVLLKLARPAILDNFVSTIDLPSYGCTIPEKTTCSIYGWGYTGLINADGLLRVAHLYIMGNEKCSQHHQGKVTLNESELCAGAEKIGSGPCEGDYGGPLICEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKVILTYKL

  • Predicted Molecular Mass

    79.3 kDa

  • Molecular Weight

    Approximately 94-99 kDa (α+β subunit) & 63-65 kDa (α subunit) & 31-34 kDa (β subunit), based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
3.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured in a cell proliferation assay using mIMCD‑3 mouse epithelial cells. The ED50 for this effect is 11.8 ng/mL, corresponding to a specific activity is 8.47×104 units/mg.

  • HGF Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for HGF Protein, Mouse (HEK293, His)
  • Experimental Validation Results for HGF Protein, Mouse (HEK293, His)
  • HGF Protein, Mouse (HEK293, His) Product Image