Wnt3a Protein, Mouse (His)

Wnt-3a protein is a Frizzled receptor ligand that activates TCF/LEF transcription factors in the canonical Wnt pathway, which is critical for embryonic mesoderm development, somite formation, neural tube morphogenesis, and stem cell self-renewal in vitro. Formation of a 1:1 complex with AFM ensures prolonged biological activity and may represent its physiological form. Wnt3a Protein, Mouse (His) is the recombinant mouse-derived Wnt3a, expressed by E. coli, with N-10*His labeled tag.

The Wnt-3a protein functions as a ligand for members of the frizzled family of seven transmembrane receptors, likely operating in the canonical Wnt signaling pathway, leading to the activation of transcription factors of the TCF/LEF family. It is crucial for normal embryonic mesoderm development, the formation of caudal somites, and the morphogenesis of the developing neural tube. Wnt-3a plays a key role in mediating the self-renewal of stem cells at the base of intestinal crypts in vitro. Additionally, the protein forms a soluble 1:1 complex with AFM, preventing oligomerization and ensuring prolonged biological activity; this complex may represent the physiological form in body fluids. As a homooligomer, Wnt-3a is disulfide-linked, leading to inactivation. It interacts with APCDD1, WLS, LRP6, PORCN, glypican GPC3, and PKD1, highlighting its involvement in diverse signaling and developmental processes.

1. Measured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED₅₀ for this effect is 3.463 ng/mL.
2. Measured by its binding ability in a functional ELISA. Immobilized LRP-5, Human at 2 μg/mL (100μL/well) can bind Biotinylated Mouse Wnt3a. The ED₅₀ for this effect is ≤6.017 ng/mL.

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  Measured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED₅₀ for this effect is 3.463 ng/mL, corresponding to a specific activity is 2.888 ×10⁵ U/mg units/mg.

Mouse

E. coli

N-10*His

P27467 (S19-K352)

22416

Full Length of Mature Protein

SYPIWWSLAVGPQYSSLSTQPILCASIPGLVPKQLRFCRNYVEIMPSVAEGVKAGIQECQHQFRGRRWNCTTVSNSLAIFGPVLDKATRESAFVHAIASAGVAFAVTRSCAEGSAAICGCSSRLQGSPGEGWKWGGCSEDIEFGGMVSREFADARENRPDARSAMNRHNNEAGRQAIASHMHLKCKCHGLSGSCEVKTCWWSQPDFRTIGDFLKDKYDSASEMVVEKHRESRGWVETLRPRYTYFKVPTERDLVYYEASPNFCEPNPETGSFGTRDRTCNVSSHGIDGCDLLCCGRGHNARTERRREKCHCVFHWCCYVSCQECTRVYDVHTCK

Approximately 39 kDa, based on SDS-PAGE under reducing conditions.

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  ≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.