Copy Link Print Information

Wnt3a Protein, Mouse (His)

5 Cited Publications
Customer Review

Based on 5 publication(s) in Google Scholar

Wnt-3a protein is a Frizzled receptor ligand that activates TCF/LEF transcription factors in the canonical Wnt pathway, which is critical for embryonic mesoderm development, somite formation, neural tube morphogenesis, and stem cell self-renewal in vitro. Formation of a 1:1 complex with AFM ensures prolonged biological activity and may represent its physiological form. Wnt3a Protein, Mouse (His) is the recombinant mouse-derived Wnt3a, expressed by E. coli, with N-10*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Wnt3a Protein, Mouse (His)
  • Experimental Validation Results for Wnt3a Protein, Mouse (His)
  • Experimental Validation Results for Wnt3a Protein, Mouse (His)
  • Experimental Validation Results for Wnt3a Protein, Mouse (His)
  • Experimental Validation Results for Wnt3a Protein, Mouse (His)
  • Experimental Validation Results for Wnt3a Protein, Mouse (His)
  • Experimental Validation Results for Wnt3a Protein, Mouse (His)
  • Experimental Validation Results for Wnt3a Protein, Mouse (His)
  • Wnt3a Protein, Mouse (His) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

Wnt-3a protein is a Frizzled receptor ligand that activates TCF/LEF transcription factors in the canonical Wnt pathway, which is critical for embryonic mesoderm development, somite formation, neural tube morphogenesis, and stem cell self-renewal in vitro. Formation of a 1:1 complex with AFM ensures prolonged biological activity and may represent its physiological form. Wnt3a Protein, Mouse (His) is the recombinant mouse-derived Wnt3a, expressed by E. coli, with N-10*His labeled tag.

Background

The Wnt-3a protein functions as a ligand for members of the frizzled family of seven transmembrane receptors, likely operating in the canonical Wnt signaling pathway, leading to the activation of transcription factors of the TCF/LEF family. It is crucial for normal embryonic mesoderm development, the formation of caudal somites, and the morphogenesis of the developing neural tube. Wnt-3a plays a key role in mediating the self-renewal of stem cells at the base of intestinal crypts in vitro. Additionally, the protein forms a soluble 1:1 complex with AFM, preventing oligomerization and ensuring prolonged biological activity; this complex may represent the physiological form in body fluids. As a homooligomer, Wnt-3a is disulfide-linked, leading to inactivation. It interacts with APCDD1, WLS, LRP6, PORCN, glypican GPC3, and PKD1, highlighting its involvement in diverse signaling and developmental processes.

Verified Bioactivity

1. Measured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 3.463 ng/mL.
2. Measured by its binding ability in a functional ELISA. Immobilized LRP-5, Human at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse Wnt3a. The ED50 for this effect is ≤6.017 ng/mL.
3. Measured by its binding ability in a functional ELISA. Immobilized Mouse Wnt3a at 2 µg/mL (100 µL/well) can bind Human LRP-6. The ED50 for this effect is 1.0-10.0 ng/mL.

Technical Parameters

  • Species Mouse
  • Source E. coli
  • Tag N-10*His
  • Accession

    P27467 (S19-K352)

  • Gene ID
    22416
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    WNT3A; Wnt Family Member 3A; Wingless‑Type MMTV Integration Site Family, Member 3A; Protein Wnt‑3a

  • AA Sequence

    SYPIWWSLAVGPQYSSLSTQPILCASIPGLVPKQLRFCRNYVEIMPSVAEGVKAGIQECQHQFRGRRWNCTTVSNSLAIFGPVLDKATRESAFVHAIASAGVAFAVTRSCAEGSAAICGCSSRLQGSPGEGWKWGGCSEDIEFGGMVSREFADARENRPDARSAMNRHNNEAGRQAIASHMHLKCKCHGLSGSCEVKTCWWSQPDFRTIGDFLKDKYDSASEMVVEKHRESRGWVETLRPRYTYFKVPTERDLVYYEASPNFCEPNPETGSFGTRDRTCNVSSHGIDGCDLLCCGRGHNARTERRREKCHCVFHWCCYVSCQECTRVYDVHTCK

  • Predicted Molecular Mass

    40.9 kDa

  • Molecular Weight

    Approximately 39 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 3.463 ng/mL, corresponding to a specific activity is 2.888 ×105 U/mg units/mg.

  • 1/16

    Wnt3a Protein, Mouse (His) purchased from MedChemExpress. Usage Cited in: CNS Neurosci Ther. 2025 Dec;31(12):e70714. [Abstract]

    Customer Validation
     The effect of intrahippocampal administration of recombinant WNT3a (200 ng/μl in 0.3 μL, on day 10 after SNI or sham operation, and then daily for 4 days) in the contralateral dorsal DG on 50% paw withdrawal threshold, dynamic score, and novel object recognition index (n = 5–6/group, Mann–Whitney nonparametric test) was evaluated.

  • 1/16

    Wnt3a Protein, Mouse (His) purchased from MedChemExpress. Usage Cited in: CNS Neurosci Ther. 2025 Dec;31(12):e70714. [Abstract]

    Customer Validation
     WNT3a treatment in SNI mice enhanced the expression of NeuroD1 and DCX. WNT3a (200 ng/μl in 0.3 μL) was administered on day 10 after SNI or sham operation and then daily for 4 days.

  • 1/16

    Wnt3a Protein, Mouse (His) purchased from MedChemExpress. Usage Cited in: CNS Neurosci Ther. 2025 Dec;31(12):e70714. [Abstract]

    Customer Validation
     WNT3a treatment in SNI mice enhanced the expression of NeuroD1 and DCX. Western blotting showed that WNT3a treatment in SNI mice enhanced the expression of p-AKT, but not total AKT.

  • 1/16

    Wnt3a Protein, Mouse (His) purchased from MedChemExpress. Usage Cited in: CNS Neurosci Ther. 2025 Dec;31(12):e70714. [Abstract]

    Customer Validation
     WNT3a treatment in SNI mice enhanced the expression of NeuroD1 and DCX. LY294002, but not JB-1, completely abolished the effects of WNT3a on the 50% paw withdrawal threshold, dynamic score, and novel object recognition index.

  • 1/16

    Wnt3a Protein, Mouse (His) purchased from MedChemExpress. Usage Cited in: J Inflamm Res. 2025 Dec 18:18:17715-17730. [Abstract]

    Customer Validation
     Wnt3a inhibited apoptosis and promoted proliferation and migration in iSCs under inflammatory conditions. TUNEL assay, microscope magnification 100x, scale bar 150μm;

  • 1/16

    Wnt3a Protein, Mouse (His) purchased from MedChemExpress. Usage Cited in: J Inflamm Res. 2025 Dec 18:18:17715-17730. [Abstract]

    Customer Validation
     Wound healing assay and quantitative analysis of the results were conducted.

  • Wnt3a Protein, Mouse (His) Product Image
    1/16
  • Experimental Validation Results for Wnt3a Protein, Mouse (His)
  • Experimental Validation Results for Wnt3a Protein, Mouse (His)
  • Experimental Validation Results for Wnt3a Protein, Mouse (His)
  • Experimental Validation Results for Wnt3a Protein, Mouse (His)
  • Experimental Validation Results for Wnt3a Protein, Mouse (His)
  • Experimental Validation Results for Wnt3a Protein, Mouse (His)
  • Experimental Validation Results for Wnt3a Protein, Mouse (His)
  • Experimental Validation Results for Wnt3a Protein, Mouse (His)
  • Wnt3a Protein, Mouse (His) Product Image

  • Wnt3a Protein, Mouse (His) purchased from MedChemExpress. Usage Cited in: CNS Neurosci Ther. 2025 Dec;31(12):e70714. [Abstract]

    The effect of intrahippocampal administration of recombinant WNT3a (200 ng/μl in 0.3 μL, on day 10 after SNI or sham operation, and then daily for 4 days) in the contralateral dorsal DG on 50% paw withdrawal threshold, dynamic score, and novel object recognition index (n = 5–6/group, Mann–Whitney nonparametric test) was evaluated.

  • Wnt3a Protein, Mouse (His) purchased from MedChemExpress. Usage Cited in: CNS Neurosci Ther. 2025 Dec;31(12):e70714. [Abstract]

    WNT3a treatment in SNI mice enhanced the expression of NeuroD1 and DCX. WNT3a (200 ng/μl in 0.3 μL) was administered on day 10 after SNI or sham operation and then daily for 4 days.

  • Wnt3a Protein, Mouse (His) purchased from MedChemExpress. Usage Cited in: CNS Neurosci Ther. 2025 Dec;31(12):e70714. [Abstract]

    WNT3a treatment in SNI mice enhanced the expression of NeuroD1 and DCX. Western blotting showed that WNT3a treatment in SNI mice enhanced the expression of p-AKT, but not total AKT.

  • Wnt3a Protein, Mouse (His) purchased from MedChemExpress. Usage Cited in: CNS Neurosci Ther. 2025 Dec;31(12):e70714. [Abstract]

    WNT3a treatment in SNI mice enhanced the expression of NeuroD1 and DCX. LY294002, but not JB-1, completely abolished the effects of WNT3a on the 50% paw withdrawal threshold, dynamic score, and novel object recognition index.

  • Wnt3a Protein, Mouse (His) purchased from MedChemExpress. Usage Cited in: J Inflamm Res. 2025 Dec 18:18:17715-17730. [Abstract]

    Wnt3a inhibited apoptosis and promoted proliferation and migration in iSCs under inflammatory conditions. TUNEL assay, microscope magnification 100x, scale bar 150μm;

  • Wnt3a Protein, Mouse (His) purchased from MedChemExpress. Usage Cited in: J Inflamm Res. 2025 Dec 18:18:17715-17730. [Abstract]

    Wound healing assay and quantitative analysis of the results were conducted.