Copy Link Print Information

HGF Protein, Mouse (HEK293)

1 Cited Publications
Customer Review

Based on 1 publication(s) in Google Scholar

HGF Protein, a potent mitogen, stimulates mature hepatocyte cell growth and acts as a hepatotrophic factor for various tissues. As an activating ligand for MET receptor tyrosine kinase, HGF binds and promotes dimerization, activating MAPK signaling through TMPRSS13 cleavage. Structurally, HGF comprises alpha and beta chains connected by a disulfide bond. Interaction with SRPX2 enhances its mitogenic activity. HGF Protein, Mouse (HEK293) is the recombinant mouse-derived HGF protein, expressed by HEK293 , with tag free.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for HGF Protein, Mouse (HEK293)
  • Experimental Validation Results for HGF Protein, Mouse (HEK293)
  • Experimental Validation Results for HGF Protein, Mouse (HEK293)
  • HGF Protein, Mouse (HEK293) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

HGF Protein, a potent mitogen, stimulates mature hepatocyte cell growth and acts as a hepatotrophic factor for various tissues. As an activating ligand for MET receptor tyrosine kinase, HGF binds and promotes dimerization, activating MAPK signaling through TMPRSS13 cleavage. Structurally, HGF comprises alpha and beta chains connected by a disulfide bond. Interaction with SRPX2 enhances its mitogenic activity. HGF Protein, Mouse (HEK293) is the recombinant mouse-derived HGF protein, expressed by HEK293 , with tag free.

Background

HGF protein is a potent mitogen that stimulates the growth of mature parenchymal hepatocyte cells and acts as a hepatotrophic factor for a wide range of tissues and cell types. It functions as an activating ligand for the receptor tyrosine kinase MET, binding to it and facilitating its dimerization. HGF also activates MAPK signaling through the cleavage and activation of TMPRSS13. Structurally, HGF is composed of an alpha chain and a beta chain joined by a disulfide bond. Additionally, it interacts with SRPX2, promoting increased mitogenic activity of HGF.

Verified Bioactivity

1.Immobilized mouse HGF at 10 μg/mL (100 μL/well) can bind mouse MET-Fc and the EC50 is 0.05-0.12 μg/mL.
2.Measured by its ability to inhibit TGFß1 activity on Mv-1-lu mink lung epithelial cells and the ED50 is typically <4 ng/mL.
3.Measured in a cell proliferation assay using mIMCD‑3 mouse epithelial cells. The ED50 for this effect is < 20 ng/mL.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag Tag Free
  • Accession

    Q08048-1 (Q33-L728)

  • Gene ID
    15234  [NCBI]
  • Molecular Construction
    • N-term
    • HGF (Q33-L728)
      Accession # Q08048-1
    • C-term
  • Protein Length

    Full Length of Isoform-1 Mature Protein

  • Synonyms

    HGF; Hepatocyte Growth Factor (Hepapoietin A; Scatter Factor); Prev. DFNB39; Lung Fibroblast-Derived Mitogen; HPTA; Hepatopoietin-A; SF; Scatter Factor; F-TCF; Deafness, Autosomal Recessive 39; HGFB; Hepatopoietin A; Hepatocyte Growth Factor; Fibroblast-D

  • AA Sequence

    QKKRRNTLHEFKKSAKTTLTKEDPLLKIKTKKVNSADECANRCIRNRGFTFTCKAFVFDKSRKRCYWYPFNSMSSGVKKGFGHEFDLYENKDYIRNCIIGKGGSYKGTVSITKSGIKCQPWNSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGPMDHTESGKTCQRWDQQTPHRHKFLPERYPDKGFDDNYCRNPDGKPRPWCYTLDPDTPWEYCAIKTCAHSAVNETDVPMETTECIQGQGEGYRGTSNTIWNGIPCQRWDSQYPHKHDITPENFKCKDLRENYCRNPDGAESPWCFTTDPNIRVGYCSQIPKCDVSSGQDCYRGNGKNYMGNLSKTRSGLTCSMWDKNMEDLHRHIFWEPDASKLNKNYCRNPDDDAHGPWCYTGNPLIPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTQTTVGWMVSLKYRNKHICGGSLIKESWVLTARQCFPARNKDLKDYEAWLGIHDVHERGEEKRKQILNISQLVYGPEGSDLVLLKLARPAILDNFVSTIDLPSYGCTIPEKTTCSIYGWGYTGLINADGLLRVAHLYIMGNEKCSQHHQGKVTLNESELCAGAEKIGSGPCEGDYGGPLICEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKVILTYKL

  • Predicted Molecular Mass

    79.3 kDa

  • Molecular Weight

    Approximately 90-95 kDa & 60-62 kDa & 30-33 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    HGF Protein, Mouse (HEK293) purchased from MedChemExpress. Usage Cited in: Small. 2024 Jun;20(25):e2308265. [Abstract]

    Customer Validation
     Western blot analysis was performed on HeLa cells treated with HGF protein (Mouse, HEK293) and varying concentrations of CD47-Met nanobody to detect the expression of Met, Akt, and Erk1/2.

  • 1/16

    HGF Protein, Mouse (HEK293) purchased from MedChemExpress. Usage Cited in: Small. 2024 Jun;20(25):e2308265. [Abstract]

    Customer Validation
     Images show HGF-induced migration of HeLa cells to the lower chamber of the Transwell system following a 24-hour treatment with CD47 nanobodies, Met aptamers, or CD47-Met nanobodies. The concentration for all treatments was 50 nM.

  • HGF Protein, Mouse (HEK293) Product Image
    1/16
  • Experimental Validation Results for HGF Protein, Mouse (HEK293)
  • Experimental Validation Results for HGF Protein, Mouse (HEK293)
  • Experimental Validation Results for HGF Protein, Mouse (HEK293)
  • HGF Protein, Mouse (HEK293) Product Image

  • HGF Protein, Mouse (HEK293) purchased from MedChemExpress. Usage Cited in: Small. 2024 Jun;20(25):e2308265. [Abstract]

    Western blot analysis was performed on HeLa cells treated with HGF protein (Mouse, HEK293) and varying concentrations of CD47-Met nanobody to detect the expression of Met, Akt, and Erk1/2.

  • HGF Protein, Mouse (HEK293) purchased from MedChemExpress. Usage Cited in: Small. 2024 Jun;20(25):e2308265. [Abstract]

    Images show HGF-induced migration of HeLa cells to the lower chamber of the Transwell system following a 24-hour treatment with CD47 nanobodies, Met aptamers, or CD47-Met nanobodies. The concentration for all treatments was 50 nM.