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Histone H3 Protein, Xenopus laevis (135a.a)

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Histone H3 Protein constitutes a core element in the nucleosome, an octamer comprising H2A, H2B, H3, and H4. The assembly forms a histone octamer with one H3-H4 heterotetramer and two H2A-H2B heterodimers, serving as a molecular spool that wraps around 147 base pairs of DNA. This compact organization contributes to chromatin structure, and Histone H3, as part of this assembly, plays a crucial role in chromatin structure and gene regulation. Histone H3 Protein, Xenopus laevis (135a.a) is the recombinant Xenopus laevis-derived Histone H3 protein, expressed by E. coli , with tag free.

For research use only. We do not sell to patients.
  • Species: Xenopus laevis
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Histone H3 Protein, Xenopus laevis (135a.a)
  • Histone H3 Protein, Xenopus laevis (135a.a) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
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Biological Activity

Description

Histone H3 Protein constitutes a core element in the nucleosome, an octamer comprising H2A, H2B, H3, and H4. The assembly forms a histone octamer with one H3-H4 heterotetramer and two H2A-H2B heterodimers, serving as a molecular spool that wraps around 147 base pairs of DNA. This compact organization contributes to chromatin structure, and Histone H3, as part of this assembly, plays a crucial role in chromatin structure and gene regulation. Histone H3 Protein, Xenopus laevis (135a.a) is the recombinant Xenopus laevis-derived Histone H3 protein, expressed by E. coli , with tag free.

Background

The Histone H3 Protein is a fundamental component of the nucleosome, which comprises a histone octamer containing two molecules each of H2A, H2B, H3, and H4. This assembly consists of one H3-H4 heterotetramer and two H2A-H2B heterodimers, collectively forming the octameric core. Functioning as a molecular spool, the histone octamer wraps approximately 147 base pairs of DNA around itself, contributing to the compact organization of chromatin. Histone H3, as part of this assembly, belongs to the histone H3 family, playing a pivotal role in chromatin structure and gene regulation.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Xenopus laevis
  • Source E. coli
  • Tag Tag Free
  • Accession

    Q92133 (A2-A136)

  • Gene ID
    399088  [NCBI]
  • Molecular Construction
    • N-term
    • Histone H3 (A2-A136)
      Accession # Q92133
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    Histone H3; h3c8.S; h3; h3.2a ; h3c8; H3l; h3r; Hist1h3f; hist1h3g; hist2h3; hist2h3c; hist1h3g.L

  • AA Sequence

    ARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEASEAYLVGLFEDTNLCGIHAKRVTIMPKDIQLARRIRGERA

  • Predicted Molecular Mass

    15.2 kDa

  • Molecular Weight

    Approximately 20 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCL, 200 mM arginine, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • Histone H3 Protein, Xenopus laevis (135a.a) Product Image
    1/16
  • Experimental Validation Results for Histone H3 Protein, Xenopus laevis (135a.a)
  • Histone H3 Protein, Xenopus laevis (135a.a) Product Image