HLA-A*02:01&B2M&p53(HMTEVVRHC) Monomer Protein, Human (Biotinylated, HEK293, His-Avi)
Based on 1 Customer Validation
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Verified Bioactivity
1.Measured by its binding ability in a functional ELISA. Immobilized Biotinylated Human HLAA*02:01&B2M&p53 (HMTEVVRHC) Complex Protein at 1 μg/mL (100 μL/well) on a Nickel Coated plate can bind Anti-HLA-A2&p53R175H Antibody, Human IgG1. The EC50 for this effect is 0.2-4 ng/mL.
2.Measured by its binding ability in a functional ELISA. Immobilized Biotinylated Human HLA-A*02:01&B2M&p53 (HMTEVVRHC) Complex Protein at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Anti-HLA-A2&p53R175H Antibody, Human IgG1. The EC50 for this effect is 0.05-2 ng/mL.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-Avi;C-His
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Accession
AAA59606.1 (G25-I308)&P61769 (I21-M119)&HMTEVVRHC
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Gene ID/&567
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Conjugation
Biotin
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Conjugate Method
The single lysine residue in Avitag is biotinylated through an enzymatic reaction.
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Synonyms
MHC; HLA-A; P53; TP53; Antigen NY-CO-13; BCC7; FLJ92943; LFS1; TRP53; Antigen NY-CO13
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Predicted Molecular Mass
49.3 kDa
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Molecular Weight
Approximately 58-65 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from 0.22 μm filtered solution of PBS, pH 7.4 with 10% trehalose as protectant.
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It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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