HLA-A*02:01&B2M&P53 WT(HMTEVVRRC) Monomer Protein, Human (Biotinylated, HEK293, His-Avi)
Based on 1 Customer Validation
HLA-A*0201 P53 WT Complex, Human (Biotinylated, HEK293, His-Avi) is a biotinylated recombinant HLA-A*0201 P53 WT complex expressed by HEK293 and tagged with C-8*His and C-Avi.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
HLA-A*0201 P53 WT Complex, Human (Biotinylated, HEK293, His-Avi) is a biotinylated recombinant HLA-A*0201 P53 WT complex expressed by HEK293 and tagged with C-8*His and C-Avi.
Background
HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen so that they can be recognized by cytotoxic T cells. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. More than 6000 HLA-A alleles have been described. The HLA system plays an important role in the occurrence and outcome of infectious diseases, including those caused by the malaria parasite, the human immunodeficiency virus (HIV), and the severe acute respiratory syndrome coronavirus (SARS-CoV).
Technical Parameters
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Species Human
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Source HEK293
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Tag C-Avi;C-8*His
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Accession
A0A140T913 (G25-T305)&P61769 (I21-M119)&HMTEVVRRC
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Conjugation
Biotin
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Synonyms
MHC; HLA-A; P53; TP53; Antigen NY-CO-13; BCC7; FLJ92943; LFS1; TRP53; Antigen NY-CO13
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AA Sequence
GSHSMRYFFTSVSRPGRGEPRFIAVGYVDDTQFVRFDSDAASQRMEPRAPWIEQEGPEYWDGETRKVKAHSQTHRVDLGTLRGYYNQSEAGSHTVQRMYGCDVGSDWRFLRGYHQYAYDGKDYIALKEDLRSWTAADMAAQTTKHKWEAAHVAEQLRAYLEGTCVEWLRRYLENGKETLQRTDAPKTHMTHHAVSDHEATLRCWALSFYPAEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVVPSGQEQRYTCHVQHEGLPKPLTLRWEPSSQPT&IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM&HMTEVVRRC
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Predicted Molecular Mass
50.5 kDa
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Molecular Weight
Approximately 51-60 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by Bis-Tris PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (255 KB)
- English - EN (255 KB)
- Français - FR (255 KB)
- Deutsch - DE (255 KB)
- Norwegian - NO (255 KB)
- Español - ES (255 KB)
- Swedish - SV (255 KB)
- Italian - IT (255 KB)
- Korean - KR (255 KB)
- Portuguese - PT (255 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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