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  3. HLA-A*0201 P53 WT Complex Protein, Human (Biotinylated, HEK293, His-Avi)

HLA-A*0201 P53 WT Complex Protein, Human (Biotinylated, HEK293, His-Avi)

Cat. No.: HY-P77779
COA Handling Instructions

HLA-A*0201 MAGE-A4 Complex Protein, a member of the major histocompatibility complex (MHC) class I family, is highlighted. Additionally, the Chimeric HLA-A*0201 WT-1 Complex Tetramer is recognized within the MHC class I family. HLA-A*0201 P53 WT Complex Protein, Human (Biotinylated, HEK293, His-Avi) is a recombinant protein dimer complex containing human-derived HLA-A*0201 P53 WT Complex protein, expressed by HEK293 , with C-Avi, C-His labeled tag. HLA-A*0201 P53 WT Complex Protein, Human (Biotinylated, HEK293, His-Avi), has molecular weight of 51-60 kDa.

For research use only. We do not sell to patients.

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Description

HLA-A*0201 MAGE-A4 Complex Protein, a member of the major histocompatibility complex (MHC) class I family, is highlighted. Additionally, the Chimeric HLA-A*0201 WT-1 Complex Tetramer is recognized within the MHC class I family. HLA-A*0201 P53 WT Complex Protein, Human (Biotinylated, HEK293, His-Avi) is a recombinant protein dimer complex containing human-derived HLA-A*0201 P53 WT Complex protein, expressed by HEK293 , with C-Avi, C-His labeled tag. HLA-A*0201 P53 WT Complex Protein, Human (Biotinylated, HEK293, His-Avi), has molecular weight of 51-60 kDa.

Background

The Chimeric HLA-A*0201 WT-1 Complex belongs to the major histocompatibility complex (MHC) class I family. The Chimeric HLA-A*0201 WT-1 Complex Tetramer is also a member of the MHC class I family.

Species

Human

Source

HEK293

Tag

C-Avi;C-His

Accession

A0A140T913 (G25-T305,HLA-A*02:01) & P61769 (I21-M119,B2M) & HMTEVVRRC

Gene ID

/&567  [NCBI]&/

Synonyms
MHC; HLA-A; P53; TP53; Antigen NY-CO-13; BCC7; FLJ92943; LFS1; TRP53
AA Sequence

GSHSMRYFFTSVSRPGRGEPRFIAVGYVDDTQFVRFDSDAASQRMEPRAPWIEQEGPEYWDGETRKVKAHSQTHRVDLGTLRGYYNQSEAGSHTVQRMYGCDVGSDWRFLRGYHQYAYDGKDYIALKEDLRSWTAADMAAQTTKHKWEAAHVAEQLRAYLEGTCVEWLRRYLENGKETLQRTDAPKTHMTHHAVSDHEATLRCWALSFYPAEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVVPSGQEQRYTCHVQHEGLPKPLTLRWEPSSQPT&IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM&HMTEVVRRC

Molecular Weight

51-60 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

HLA-A*0201 P53 WT Complex Protein, Human (Biotinylated, HEK293, His-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HLA-A*0201 P53 WT Complex Protein, Human (Biotinylated, HEK293, His-Avi)
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HY-P77779
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