HNF4A Protein, Human (HEK293, His, solution)

HNF 4 alpha, a nuclear transcription factor, forms homodimers to bind DNA. It regulates gene expression, including hepatocyte nuclear factor 1 alpha, impacting hepatic genes. Its involvement extends to liver, kidney, and intestinal development. Mutations associate with autosomal dominant non-insulin-dependent diabetes mellitus type I. The gene exhibits biased expression in the duodenum (RPKM 37.7) and colon (RPKM 32.8), among other tissues. HNF4A Protein, Human (HEK293, His, solution) is the recombinant human-derived HNF 4 alpha, expressed by HEK293 , with C-10*His labeled tag.

HNF 4 alpha, the protein product of this gene, functions as a nuclear transcription factor, forming homodimers to bind DNA and exerting control over the expression of various genes, including hepatocyte nuclear factor 1 alpha, a key regulator of hepatic gene expression. Its involvement extends to the development of vital organs such as the liver, kidney, and intestines. Mutations in HNF 4 alpha have been linked to monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I, underscoring its significance in metabolic regulation. Additionally, alternative splicing yields multiple transcript variants, generating diverse isoforms. Notably, HNF 4 alpha displays biased expression, with higher levels observed in the duodenum (RPKM 37.7), colon (RPKM 32.8), and seven other tissues, emphasizing its specialized role in diverse physiological contexts.

Human

HEK293

C-10*His

P41235-1/NP_000448 (M1-I474)

3172

Full Length of Isoform-1

MRLSKTLVDMDMADYSAALDPAYTTLEFENVQVLTMGNDTSPSEGTNLNAPNSLGVSALCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTRRSSYEDSSLPSINALLQAEVLSRQITSPVSGINGDIRAKKIASIADVCESMKEQLLVLVEWAKYIPAFCELPLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLLGNDYIVPRHCPELAEMSRVSIRILDELVLPFQELQIDDNEYAYLKAIIFFDPDAKGLSDPGKIKRLRSQVQVSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQMIEQIQFIKLFGMAKIDNLLQEMLLGGSPSDAPHAHHPLHPHLMQEHMGTNVIVANTMPTHLSNGQMCEWPRPRGQAATPETPQPSPPGGSGSEPYKLLPGAVATIVKPLSAIPQPTITKQEVI

Approximately 70-80 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 10% glycerol, pH 7.3.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.