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HNRNPM Protein, Human (N-His)

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Based on 1 Customer Validation

The HNRNPM protein is a key pre-mRNA binding protein that exhibits strong affinity for poly(G) and poly(U) RNA homopolymers. It is central to the splicing process, complexly regulates RNA maturation, and serves as a receptor for carcinoembryonic antigen in Kupffer cells. HNRNPM Protein, Human (N-His) is the recombinant human-derived HNRPM protein, expressed by E. coli , with N-10*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for HNRNPM Protein, Human (N-His)
  • HNRNPM Protein, Human (N-His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The HNRNPM protein is a key pre-mRNA binding protein that exhibits strong affinity for poly(G) and poly(U) RNA homopolymers. It is central to the splicing process, complexly regulates RNA maturation, and serves as a receptor for carcinoembryonic antigen in Kupffer cells. HNRNPM Protein, Human (N-His) is the recombinant human-derived HNRPM protein, expressed by E. coli , with N-10*His labeled tag.

Background

The HNRNPM protein emerges as a crucial pre-mRNA binding protein in vivo, exhibiting a strong affinity for poly(G) and poly(U) RNA homopolymers in vitro. Functionally implicated in splicing processes, HNRNPM plays a significant role in the intricate regulation of RNA maturation. Beyond its involvement in splicing, it acts as a receptor for carcinoembryonic antigen in Kupffer cells, potentially initiating a cascade of signaling events leading to the tyrosine phosphorylation of proteins and the induction of cytokines such as IL-1 alpha, IL-6, IL-10, and tumor necrosis factor alpha. Notably, HNRNPM is identified as a component of the spliceosome C complex, underscoring its association with fundamental RNA processing machinery. Moreover, the protein interacts with PPIA/CYPA, further expanding its network of molecular interactions and highlighting its multifaceted role in cellular processes.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-10*His
  • Accession

    P52272-2 (M1-A691)

  • Gene ID
    4670
  • Protein Length

    Full Length of Isoform-2

  • Synonyms

    HNRNPM; CEAR; Prev. HNRPM; HnRNP M; Prev. NAGR1; Heterogenous Nuclear Ribonucleoprotein M4; HNRNPM4; N-Acetylglucosamine Receptor 1; HNRPM4; HnRNA-Binding Protein M4; HTGR1; Heterogeneous Nuclear Ribonucleoprotein M; CEA Receptor

  • AA Sequence

    MAAGVEAAAEVAATEIKMEEESGAPGVPSGNGAPGPKGEGERPAQNEKRKEKNIKRGGNRFEPYANPTKRYRAFITNIPFDVKWQSLKDLVKEKVGEVTYVELLMDAEGKSRGCAVVEFKMEESMKKAAEVLNKHSLSGRPLKVKEDPDGEHARRAMQKAGRLGSTVFVANLDYKVGWKKLKEVFSMAGVVVRADILEDKDGKSRGIGTVTFEQSIEAVQAISMFNGQLLFDRPMHVKMDERALPKGDFFPPERPQQLPHGLGGIGMGLGPGGQPIDANHLNKGIGMGNIGPAGMGMEGIGFGINKMGGMEGPFGGGMENMGRFGSGMNMGRINEILSNALKRGEIIAKQGGGGGGGSVPGIERMGPGIDRLGGAGMERMGAGLGHGMDRVGSEIERMGLVMDRMGSVERMGSGIERMGPLGLDHMASSIERMGQTMERIGSGVERMGAGMGFGLERMAAPIDRVGQTIERMGSGVERMGPAIERMGLSMERMVPAGMGAGLERMGPVMDRMATGLERMGANNLERMGLERMGANSLERMGLERMGANSLERMGPAMGPALGAGIERMGLAMGGGGGASFDRAIEMERGNFGGSFAGSFGGAGGHAPGVARKACQIFVRNLPFDFTWKMLKDKFNECGHVLYADIKMENGKSKGCGVVKFESPEVAERACRMMNGMKLSGREIDVRIDRNA

  • Predicted Molecular Mass

    73.6 kDa

  • Molecular Weight

    Approximately 75 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • HNRNPM Protein, Human (N-His) Product Image
    1/16
  • Experimental Validation Results for HNRNPM Protein, Human (N-His)
  • HNRNPM Protein, Human (N-His) Product Image