HPD/HPPDase Protein, Human (His)

HPD is a key enzyme in tyrosine catabolism and plays a key role in catalyzing the conversion of 4-hydroxyphenylpyruvate into homogentisate. This enzymatic process represents a key step in the tyrosine metabolic pathway and contributes to the breakdown of this amino acid. HPD/HPPDase Protein, Human (His) is the recombinant human-derived HPD/HPPDase protein, expressed by E. coli , with N-6*His labeled tag. The total length of HPD/HPPDase Protein, Human (His) is 393 a.a., with molecular weight of 40-50 kDa.

HPD, also known as 4-hydroxyphenylpyruvate dioxygenase or HPPDase, is a crucial enzyme in tyrosine catabolism. It plays a pivotal role in the conversion of 4-hydroxyphenylpyruvic acid to homogentisic acid, representing a key step in the degradation pathway of tyrosine. Through its catalytic activity, HPD facilitates the transformation of 4-hydroxyphenylpyruvic acid into homogentisic acid, contributing to the breakdown of tyrosine and the subsequent metabolism of aromatic amino acids. This enzymatic process is essential for maintaining the balance of tyrosine-derived metabolites and is integral to various physiological processes within the cell (adapted from the provided passage).

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

P32754 (M1-M393)

3242  [NCBI]

MTTYSDKGAKPERGRFLHFHSVTFWVGNAKQAASFYCSKMGFEPLAYRGLETGSREVVSHVIKQGKIVFVLSSALNPWNKEMGDHLVKHGDGVKDIAFEVEDCDYIVQKARERGAKIMREPWVEQDKFGKVKFAVLQTYGDTTHTLVEKMNYIGQFLPGYEAPAFMDPLLPKLPKCSLEMIDHIVGNQPDQEMVSASEWYLKNLQFHRFWSVDDTQVHTEYSSLRSIVVANYEESIKMPINEPAPGKKKSQIQEYVDYNGGAGVQHIALKTEDIITAIRHLRERGLEFLSVPSTYYKQLREKLKTAKIKVKENIDALEELKILVDYDEKGYLLQIFTKPVQDRPTLFLEVIQRHNHQGFGAGNFNSLFKAFEEEQNLRGNLTNMETNGVVPGM

40-50 kDa

Greater than 95% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 20% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.