Hyaluronidase 2/HYAL2 Protein, Mouse (HEK293, His)
Hyaluronidase 2/HYAL2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Hyaluronidase 2/HYAL2 protein, expressed by HEK293, with C-His tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Biological Activity
Description
Hyaluronidase 2/HYAL2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Hyaluronidase 2/HYAL2 protein, expressed by HEK293, with C-His tag.
Background
Hyaluronidase 2 (HYAL2) protein is involved in the hydrolysis of high molecular weight hyaluronic acid, generating intermediate-sized products that are subsequently processed by sperm hyaluronidase to produce small oligosaccharides. Although HYAL2 exhibits very low levels of activity, its role in the breakdown of hyaluronic acid suggests a potential contribution to the modulation of extracellular matrix dynamics. Additionally, HYAL2 associates with and negatively regulates MST1R, indicating a potential regulatory role in cellular processes mediated by MST1R.
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-8*His
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Accession
O35632 (L23-N448)
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Gene ID15587
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Protein Length
Full Length of Mature Protein
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Synonyms
HYAL2; Hyaluronidase-2; Hyaluronidase 2; PH-20 Homolog; LUCA2; Hyal-2; Hyaluronoglucosaminidase 2; Hyaluronoglucosaminidase-2; LuCa-2; Hyaluronidase; Lung Carcinoma Protein 2; PH20 Homolog; Lysosomal Hyaluronidase; MCCS
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AA Sequence
LKPTAPPIFTGRPFVVAWNVPTQECAPRHKVPLDLRAFDVKATPNEGFFNQNITTFYYDRLGLYPRFDAAGTSVHGGVPQNGSLCAHLPMLKESVERYIQTQEPGGLAVIDWEEWRPVWVRNWQEKDVYRQSSRQLVASRHPDWPSDRVMKQAQYEFEFAARQFMLNTLRYVKAVRPQHLWGFYLFPDCYNHDYVQNWESYTGRCPDVEVARNDQLAWLWAESTALFPSVYLDETLASSVHSRNFVSFRVREALRVAHTHHANHALPVYVFTRPTYTRGLTGLSQVDLISTIGESAALGSAGVIFWGDSEDASSMETCQYLKNYLTQLLVPYIVNVSWATQYCSWTQCHGHGRCVRRNPSANTFLHLNASSFRLVPGHTPSEPQLRPEGQLSEADLNYLQKHFRCQCYLGWGGEQCQRNYKGAAGN
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Predicted Molecular Mass
49.9 kDa
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Molecular Weight
Approximately 52-68 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)