ICAM-1/CD54 Protein, Rhesus Macaque (HEK293, His)
The ICAM-1/CD54 protein is a ligand of the leukocyte adhesion protein LFA-1 and is critical for leukocyte transendothelial migration. Its involvement activates ARHGEF26/SGEF and RHOG, coordinating endothelial apical cup assembly. ICAM-1/CD54 Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived ICAM-1/CD54 protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Rhesus Macaque
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
The ICAM-1/CD54 protein is a ligand of the leukocyte adhesion protein LFA-1 and is critical for leukocyte transendothelial migration. Its involvement activates ARHGEF26/SGEF and RHOG, coordinating endothelial apical cup assembly. ICAM-1/CD54 Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived ICAM-1/CD54 protein, expressed by HEK293 , with C-6*His labeled tag.
Background
ICAM-1/CD54 Protein, as a ligand for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2), plays a crucial role in leukocyte trans-endothelial migration. Engagement of ICAM-1 during this process promotes the assembly of endothelial apical cups through the activation of ARHGEF26/SGEF and RHOG, revealing its involvement in orchestrating dynamic cellular events (By similarity). ICAM-1 also forms homodimers and interacts with MUC1, fostering cell aggregation in epithelial cells. Furthermore, its interaction with ARHGEF26/SGEF underscores its participation in signaling pathways that regulate cellular dynamics. On the T cell side, ICAM-1 interacts with CD81, CD247, and CD9 at immunological synapses between antigen-presenting cells and T cells, emphasizing its pivotal role in immune cell interactions during the formation of immunological synapses. The multifaceted interactions of ICAM-1 highlight its significance in mediating diverse cellular processes and facilitating cell-cell communication.
Technical Parameters
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Species Rhesus Macaque
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Source HEK293
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Tag C-6*His
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Accession
Q5NKV6 (Q28-E480)
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Molecular Construction
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N-term
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ICAM-1 (Q28-E480)
Accession # Q5NKV6 -
6*His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
ICAM1; Epididymis Secretory Sperm Binding Protein; Intercellular Adhesion Molecule 1; Intercellular Adhesion Molecule-1; CD54; Cell Surface Glycoprotein P3.58; BB2; Human Rhinovirus Receptor; Major Group Rhinovirus Receptor; CD54 Antigen; ICAM-1; P3.58; I
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AA Sequence
QTSVFPPEVILPRGGSVKVNCSASCDQPISLGMETPLPKKEILPGGNNWKMYELSNVQEDSQPMCYSNCPDGQSSAKTLLTVYWTPERVELAPLPPWQPVGKNLTLRCQVEGGAPRANLTVMLLRGEKELSRQSAVGEPAEVTTTVPVGRDDHGANFSCRTELDLRPYVLKLFENTSAPHQLQTFDLPATPPQLVSPQVLEVDTQGTVVCSLDGLFPVSEAQVSLALGDQKLNPTITYGNNSLSAKASVKVTAEEEGTQQLLCGVMLGNQTQETRQTVTIYSFPAPNVNLTKPEVSEGTEVIVECEAHPRAKVMLNGVPAQPPGPRAQFLLKATPEDNGRSFSCSATLEVAGQLVHKNQTRELRVLYGPRLDEKDCPGNWTWPENSQQTPMCQAWGNPLPQLKCLKDGTFPLPIGQSVTVTRDLEGTYLCQARSTRGEVTREVTVNVLSPRYE
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Predicted Molecular Mass
50.2 kDa
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Molecular Weight
Approximately 70-115 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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