ICAM-5 Protein, Mouse (HEK293, His)
Based on 1 Customer Validation
ICAM-5 protein, acting as a ligand for LFA-1, plays a crucial role in cell adhesion, specifically facilitating leukocyte binding to target cells. This interaction is integral to immune responses and inflammation, influencing immune cell recruitment and system modulation. ICAM-5's specificity for LFA-1 underscores its involvement in regulating leukocyte adhesion, a fundamental aspect of immune cell behavior and system functionality. ICAM-5 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ICAM-5 protein, expressed by HEK293 , with C-10*His labeled tag and P47R mutation.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
ICAM-5 protein, acting as a ligand for LFA-1, plays a crucial role in cell adhesion, specifically facilitating leukocyte binding to target cells. This interaction is integral to immune responses and inflammation, influencing immune cell recruitment and system modulation. ICAM-5's specificity for LFA-1 underscores its involvement in regulating leukocyte adhesion, a fundamental aspect of immune cell behavior and system functionality. ICAM-5 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ICAM-5 protein, expressed by HEK293 , with C-10*His labeled tag and P47R mutation.
Background
ICAM-5 protein holds significance as a ligand for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). This interaction underscores ICAM-5's crucial role in mediating cell adhesion processes, particularly facilitating the binding of leukocytes to target cells. The engagement between ICAM-5 and LFA-1 is integral to immune responses and inflammatory reactions, where proper cell-cell interactions are vital for immune cell recruitment and modulation of immune system functions. The specificity of ICAM-5 for LFA-1 highlights its involvement in regulating leukocyte adhesion, a fundamental aspect of immune cell behavior and immune system functionality.
Verified Bioactivity
Measured by its ability of the immobilized protein to support the adhesion of PMA-stimulated Jurkat cells. When 5×104 cells/well are added to ICAM-5 coated plates (25 μg/mL,100 μL/well), 46.215% will adhere after 1 hour at 37°C.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-10*His
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Accession
Q60625 (L31-R828, P47R)
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Gene ID15898
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Molecular Construction
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N-term
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ICAM-5 (L31-R828, P47R)
Accession # Q60625 -
10*His
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C-term
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Protein Length
Partial Extracellular Domain
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Synonyms
ICAM5; TLN; Prev. TLCN; Intercellular Adhesion Molecule 5, Telencephalin; Telencephalin; ICAM5 Protein; Intercellular Adhesion Molecule 5; ICAM-5
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AA Sequence
LEPFWADLQPRVALVERGGSLWLNCSTNCPRPERGGLETSLRRNGTQRGLRWLARQLVDIREPETQPVCFFRCARRTLQARGLIRTFQRPDRVELVPLPSWQPVGENFTLSCRVPGAGPRASLTLTLLRGGQELIRRSFVGEPPRARGAMLTARVLARREDHRVNFSCLAELDLRPHGLGLFANSSAPRQLRTFAMPPHSPSLIAPRVLEVDSERPVTCTLDGLFPAPEAGVYLSLGDQRLNPNVTLDGDSLVATATATASAEQEGTKQLMCVVTLGGETRETQENLTVYSFPTPLLTLSEPEAPEGKMVTISCWAGARALVTLEGIPAAVPGQPAELQLNVTKNDDKRGFFCDAALDVDGETLRKNQSSELRVLYAPRLDDLDCPRSWTWPEGPEQTLHCEARGNPEPSVHCARPEGGAVLALGLLGPVTRALAGTYRCTAVNGQGQAVKDVTLTVEYAPALDSVGCPEHITWLEGTEASLSCVAPGVPPPSVSCVRSGKEEVMEGPLRVAREHAGTYRCEAINARGSAAKNVAVTVEYGPSFEELGCPSNWTWVEGSGKLFSCEVDGKPEPRVECVGSEGASEGIVLPLVSSNSGPRNSMTPGNLSPGIYLCNATNRHGSTVKTVVVSAESPPQMDESSCPSHQTWLEGAEATALACSARGRPSPRVHCSREGAARLERLQVSREDAGTYRCVATNAHGTDSRTVTVGVEYRPVVAELAASPPSVRPGGNFTLTCRAEAWPPAQISWRAPPGALNLGLSSNNSTLSVAGAMGSHGGEYECAATNAHGRHARRITVR
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Predicted Molecular Mass
85.6 kDa
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Molecular Weight
Approximately 130-140 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-Citrate, 300 mM NaCl, pH 7.4, 8% trehalose.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (240 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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