1. Recombinant Proteins
  2. Fc Receptors Biotinylated Proteins
  3. Immunoglobulin Fc Region
  4. IgG3
  5. IgG3 Fc Protein, Human (Biotinylated, HEK293, Avi)

IgG3 Fc Protein, Human (Biotinylated, HEK293, Avi)

Art. -Nr.: HY-P705220
Handling Instructions Technical Support

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  • Biologische Aktivität

  • Technical Parameters

  • Properties

  • Beschreibung

Biologische Aktivität

Measured by its binding ability in a functional ELISA.Immobilized Human CD64, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human IgG3 Fc Protein, Avitag.

Species

Human

Source

HEK293

Tag

N-Avi

Accession

P01860-1 (E99-K377)

Gene ID

3502

Molecular Construction
N-term
Avi
IgG3 Fc (E99-K377)
Accession # P01860-1
C-term
Protein Length

Partial

Conjugation

Biotin

Synonyms
Ig gamma-3 chain C region; IGHG3; IgG3 Fc; HDC
AA Sequence

ELKTPLGDTTHTCPRCPEPKSCDTPPPCPRCPEPKSCDTPPPCPRCPEPKSCDTPPPCPRCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVQFKWYVDGVEVHNAKTKPREEQYNSTFRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKTKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESSGQPENNYNTTPPMLDSDGSFFLYSKLTVDKSRWQQGNIFSCSVMHEALHNRFTQKSLSLSPGK

Predicted Molecular Mass
33.4 kDa.
Molekulargewicht

Approximately 38-43 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

Glycosylation
Yes
Reinheit

≥ 95%, as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from 0.22 μm filtered solution of PBS, pH7.4 with 10% trehalose as protectant.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Versand

Room temperature in continental US; may vary elsewhere.

Dokumentation

IgG3 Fc Protein, Human (Biotinylated, HEK293, Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IgG3 Fc Protein, Human (Biotinylated, HEK293, Avi)

 

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Produktname:
IgG3 Fc Protein, Human (Biotinylated, HEK293, Avi)
Art. -Nr.:
HY-P705220
Menge:
MCE Japan Authorized Agent: