IkB alpha Protein, Human (His)

IκB α is a key cellular regulator that inhibits NF-κ-B/REL complexes by sequestering them in the cytoplasm, thereby impeding nuclear translocation and transcriptional activation. It masks the nuclear localization signals of RELA/p65 and NFKB1/p50. IkB alpha Protein, Human (His) is the recombinant human-derived IkB alpha protein, expressed by E. coli , with N-His labeled tag. The total length of IkB alpha Protein, Human (His) is 316 a.a., with molecular weight of ~38 kDa.

IkB alpha, a pivotal regulator in cellular responses, exerts its inhibitory influence by sequestering dimeric NF-kappa-B/REL complexes in the cytoplasm, thereby impeding their nuclear translocation and subsequent activation of transcription. This intricate control involves masking the nuclear localization signals of RELA/p65 and NFKB1/p50. Upon cellular stimulation by immune and pro-inflammatory cues, IkB alpha undergoes phosphorylation, triggering ubiquitination and degradation. This orchestrated process liberates the dimeric RELA, allowing its translocation to the nucleus for transcriptional activation. IkB alpha engages in a complex interplay with various proteins, such as RELA, NKIRAS1, NKIRAS2, RWDD3 isoforms, PRMT2, PRKACA, MEFV, and DDRGK1, highlighting its versatile interactions and regulatory roles in diverse cellular pathways.

Human

E. coli

N-His

P25963/NP_065390.1 (F2-L317)

4792  [NCBI]

Full length

FQAAERPQEWAMEGPRDGLKKERLLDDRHDSGLDSMKDEEYEQMVKELQEIRLEPQEVPRGSEPWKQQLTEDGDSFLHLAIIHEEKALTMEVIRQVKGDLAFLNFQNNLQQTPLHLAVITNQPEIAEALLGAGCDPELRDFRGNTPLHLACEQGCLASVGVLTQSCTTPHLHSILKATNYNGHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNGRTALHLAVDLQNPDLVSLLLKCGADVNRVTYQGYSPYQLTWGRPSTRIQQQLGQLTLENLQMLPESEDEESYDTESEFTEFTEDELPYDDCVFGGQRLTL

Approximately 38 kDa, based on SDS-PAGE under reducing conditions.

≥ 90%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of50 mM Tris, 0.5 M NaCl, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.