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IkB alpha Protein, Human (His)

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IκB α is a key cellular regulator that inhibits NF-κ-B/REL complexes by sequestering them in the cytoplasm, thereby impeding nuclear translocation and transcriptional activation. It masks the nuclear localization signals of RELA/p65 and NFKB1/p50. IkB alpha Protein, Human (His) is the recombinant human-derived IkB alpha protein, expressed by E. coli , with N-His labeled tag. The total length of IkB alpha Protein, Human (His) is 316 a.a., with molecular weight of ~38 kDa.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
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Biological Activity

Description

IκB α is a key cellular regulator that inhibits NF-κ-B/REL complexes by sequestering them in the cytoplasm, thereby impeding nuclear translocation and transcriptional activation. It masks the nuclear localization signals of RELA/p65 and NFKB1/p50. IkB alpha Protein, Human (His) is the recombinant human-derived IkB alpha protein, expressed by E. coli , with N-His labeled tag. The total length of IkB alpha Protein, Human (His) is 316 a.a., with molecular weight of ~38 kDa.

Background

IkB alpha, a pivotal regulator in cellular responses, exerts its inhibitory influence by sequestering dimeric NF-kappa-B/REL complexes in the cytoplasm, thereby impeding their nuclear translocation and subsequent activation of transcription. This intricate control involves masking the nuclear localization signals of RELA/p65 and NFKB1/p50. Upon cellular stimulation by immune and pro-inflammatory cues, IkB alpha undergoes phosphorylation, triggering ubiquitination and degradation. This orchestrated process liberates the dimeric RELA, allowing its translocation to the nucleus for transcriptional activation. IkB alpha engages in a complex interplay with various proteins, such as RELA, NKIRAS1, NKIRAS2, RWDD3 isoforms, PRMT2, PRKACA, MEFV, and DDRGK1, highlighting its versatile interactions and regulatory roles in diverse cellular pathways.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-His
  • Accession

    P25963/NP_065390.1 (F2-L317)

  • Gene ID
    4792  [NCBI]
  • Molecular Construction
    • N-term
    • His
    • IkB alpha (F2-L317)
      Accession # P25963/NP_065390.1
    • C-term
  • Protein Length

    Full length

  • Synonyms

    NFKBIA; MAD-3; Prev. NFKBI; Major Histocompatibility Complex Enhancer-Binding Protein MAD3; NF-Kappa-B Inhibitor Alpha; IkB-Alpha; IkappaBalpha; Nuclear Factor Of Kappa Light Chain Gene Enhancer In B-Cells; IKBA; EDAID2; I-Kappa-B-Alpha; MAD3; NFKB Inhibi

  • AA Sequence

    FQAAERPQEWAMEGPRDGLKKERLLDDRHDSGLDSMKDEEYEQMVKELQEIRLEPQEVPRGSEPWKQQLTEDGDSFLHLAIIHEEKALTMEVIRQVKGDLAFLNFQNNLQQTPLHLAVITNQPEIAEALLGAGCDPELRDFRGNTPLHLACEQGCLASVGVLTQSCTTPHLHSILKATNYNGHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNGRTALHLAVDLQNPDLVSLLLKCGADVNRVTYQGYSPYQLTWGRPSTRIQQQLGQLTLENLQMLPESEDEESYDTESEFTEFTEDELPYDDCVFGGQRLTL

  • Predicted Molecular Mass

    36.4 kDa

  • Molecular Weight

    Approximately 38 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of50 mM Tris, 0.5 M NaCl, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

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