IL-18 Protein, Rhesus Macaque
IL-18 protein is a pro-inflammatory cytokine that is critically involved in epithelial barrier repair and regulates immune responses, especially Th1 cell and NK cell immune responses. IL-18 binds to IL18R1 and IL18RAP receptors to form a signaling ternary complex, activate NF-κ-B, and induce inflammatory mediators. IL-18 Protein, Rhesus Macaque is the recombinant Rhesus Macaque-derived IL-18 protein, expressed by E. coli.
- Species: Rhesus Macaque
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
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Biological Activity
Description
IL-18 protein is a pro-inflammatory cytokine that is critically involved in epithelial barrier repair and regulates immune responses, especially Th1 cell and NK cell immune responses. IL-18 binds to IL18R1 and IL18RAP receptors to form a signaling ternary complex, activate NF-κ-B, and induce inflammatory mediators. IL-18 Protein, Rhesus Macaque is the recombinant Rhesus Macaque-derived IL-18 protein, expressed by E. coli.
Background
IL-18 Protein is a pro-inflammatory cytokine that plays a crucial role in epithelial barrier repair and the modulation of immune responses, specifically in polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to its receptors IL18R1 and IL18RAP, IL-18 forms a signaling ternary complex that activates NF-kappa-B, resulting in the synthesis of inflammatory mediators. It also works synergistically with IL-12/interleukin-12 to induce the synthesis of IFNG from Th1 cells and NK cells. Furthermore, IL-18 is involved in transducing inflammation downstream of pyroptosis, where its mature form is selectively released into the extracellular space through the gasdermin-D (GSDMD) pore.
Technical Parameters
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Species Rhesus Macaque
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Source E. coli
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Tag Tag Free
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Accession
Q9BGI5/NP_001028006.1 (Y37-D193)
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Gene ID574151
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Molecular Construction
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N-term
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IL-18 (Y37-D193)
Accession # Q9BGI5/NP_001028006.1 -
C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
IL18; Interleukin 18 (Interferon-Gamma-Inducing Factor); Interleukin 18; IFN-Gamma-Inducing Factor; IL-18; Interleukin-1 Gamma; IL1F4; Iboctadekin; IGIF; IL-1 Gamma; Interleukin-18; Interferon-Gamma-Inducing Factor; IL-1g; Interferon Gamma-Inducing Factor
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AA Sequence
AAEPAEDNCINFVAMKFIDSTLYFIAEDDENLESDYFGKLESKLSIIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIINMYKDSQPRGMAVAISVKCEKISTLSCENRIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLYKLILKKKDELGDRSIMFTVQNED
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Predicted Molecular Mass
18.3 kDa
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Molecular Weight
Approximately 15-17 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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