IL-2 Protein, Mouse (Biotinylated, HEK293, His-Avi)

IL-2 Protein is usually produces by antigen-activated CD4+ T cells, CD8+ T cells, NK cells and NKT cells. IL-2 Protein is involved in signaling pathways including JAK/STAT, inosine phosphate 3-kinase /PI3K, and mitogen-activated protein kinase /MAPK. IL-2 Protein, Mouse (Biotinylated, HEK293, His-Avi) is the recombinant mouse-derived IL-2 protein, expressed by HEK293 , with N-His, N-Avi labeled tag.

IL-2 is a receptor cytokine produced by activated CD4-positive helper T cells and plays its role by activating JAK/STAT, inosine phosphate 3-kinase /PI3K and mitogen-activated protein kinase /MAPK. IL-2 binds to receptor complexes consisting of high affinity trimers IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or low affinity dimers IL-2R (IL2RB and IL2RG). IL-2 can increase the cytolytic activity of NK cells. Promote strong proliferation of activated B cells and immunoglobulin production. IL-2 is involved in differentiation and homeostasis of effector T cell subsets, including Th1, Th2, Th17, and memory CD8-positive T cells. IL-2 synthesis is strictly regulated by TCR and CD28 signaling at the mRNA level. It mediates the activation induced cell death (AICD) process. IL-2 can be used in the research of cancer immunotherapy^{[1][2][3][4][5][6]}.

1.Immobilized Mouse IL-2 R alpha, His Tag at 2 μg/mL (100 μL/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC₅₀ of 67.3-95.8 ng/mL determined by ELISA.
2.Immobilized Mouse IL-2 R alpha, His Tag at 5 μg/mL (100 μL/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC₅₀ of 0.1-1.0 μg/mL determined by ELISA.

Mouse

HEK293

N-Avi;N-8*His

P04351 (A21-Q169)

16183  [NCBI]

Full Length of Mature Protein

Biotin

The single lysine residue in Avitag is biotinylated through an enzymatic reaction.

APTSSSTSSSTAEAQQQQQQQQQQQQHLEQLLMDLQELLSRMENYRNLKLPRMLTFKFYLPKQATELKDLQCLEDELGPLRHVLDLTQSKSFQLEDAENFISNIRVTVVKLKGSDNTFECQFDDESATVVDFLRRWIAFCQSIISTSPQ

Approximately 27-30 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

≥ 95%, as determined by Bis-Tris PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. /biology-dictionary/baf3-cell.html  [Content Brief]

  [2]. G Reya T, et al. Thymic stromal-cell abnormalities and dysregulated T-cell development in IL-2-deficient mice. Dev Immunol. 1998;5(4):287-302.  [Content Brief]

  [3]. Zhu J, et al. Stat5 activation plays a critical role in Th2 differentiation. Immunity. 2003 Nov;19(5):739-48.  [Content Brief]

  [4]. Smith GA, et al. Essential biphasic role for JAK3 catalytic activity in IL-2 receptor signaling. Nat Chem Biol. 2016 May;12(5):373-9.  [Content Brief]

  [5]. Sim GC, et al. The IL-2 cytokine family in cancer immunotherapy. Cytokine Growth Factor Rev. 2014 Aug;25(4):377-90.  [Content Brief]

  [6]. Kim HP, et al. Both integrated and differential regulation of components of the IL-2/IL-2 receptor system. Cytokine Growth Factor Rev. 2006 Oct;17(5):349-66.  [Content Brief]

  [7]. Nelson BH. IL-2, regulatory T cells, and tolerance. J Immunol. 2004 Apr 1;172(7):3983-8.  [Content Brief]