IL-2 Protein, Mouse (Biotinylated, HEK293, His-Avi)
Based on 1 Customer Validation
IL-2 Protein is usually produces by antigen-activated CD4+ T cells, CD8+ T cells, NK cells and NKT cells. IL-2 Protein is involved in signaling pathways including JAK/STAT, inosine phosphate 3-kinase /PI3K, and mitogen-activated protein kinase /MAPK. IL-2 Protein, Mouse (Biotinylated, HEK293, His-Avi) is the recombinant mouse-derived IL-2 protein, expressed by HEK293 , with N-His, N-Avi labeled tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
IL-2 Protein is usually produces by antigen-activated CD4+ T cells, CD8+ T cells, NK cells and NKT cells. IL-2 Protein is involved in signaling pathways including JAK/STAT, inosine phosphate 3-kinase /PI3K, and mitogen-activated protein kinase /MAPK. IL-2 Protein, Mouse (Biotinylated, HEK293, His-Avi) is the recombinant mouse-derived IL-2 protein, expressed by HEK293 , with N-His, N-Avi labeled tag.
Background
IL-2 is a receptor cytokine produced by activated CD4-positive helper T cells and plays its role by activating JAK/STAT, inosine phosphate 3-kinase /PI3K and mitogen-activated protein kinase /MAPK. IL-2 binds to receptor complexes consisting of high affinity trimers IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or low affinity dimers IL-2R (IL2RB and IL2RG). IL-2 can increase the cytolytic activity of NK cells. Promote strong proliferation of activated B cells and immunoglobulin production. IL-2 is involved in differentiation and homeostasis of effector T cell subsets, including Th1, Th2, Th17, and memory CD8-positive T cells. IL-2 synthesis is strictly regulated by TCR and CD28 signaling at the mRNA level. It mediates the activation induced cell death (AICD) process. IL-2 can be used in the research of cancer immunotherapy[1][2][3][4][5][6].
Verified Bioactivity
1.Immobilized Mouse IL-2 R alpha, His Tag at 2 μg/mL (100 μL/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 67.3-95.8 ng/mL determined by ELISA.
2.Immobilized Mouse IL-2 R alpha, His Tag at 5 μg/mL (100 μL/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 0.1-1.0 μg/mL determined by ELISA.
Technical Parameters
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Species Mouse
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Source HEK293
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Tag N-Avi;N-8*His
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Accession
P04351 (A21-Q169)
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Molecular Construction
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N-term
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8*His-Avi
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IL-2 (A21-Q169)
Accession # P04351 -
C-term
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Protein Length
Full Length of Mature Protein
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Conjugation
Biotin
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Conjugate Method
The single lysine residue in Avitag is biotinylated through an enzymatic reaction.
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Synonyms
IL2; T Cell Growth Factor; Interleukin 2; Aldesleukin; IL-2; Involved In Regulation Of T-Cell Clonal Expansion; TCGF; T-Cell Growth Factor; Interleukin-2; Lymphokine
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AA Sequence
APTSSSTSSSTAEAQQQQQQQQQQQQHLEQLLMDLQELLSRMENYRNLKLPRMLTFKFYLPKQATELKDLQCLEDELGPLRHVLDLTQSKSFQLEDAENFISNIRVTVVKLKGSDNTFECQFDDESATVVDFLRRWIAFCQSIISTSPQ
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Predicted Molecular Mass
20.1 kDa
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Molecular Weight
Approximately 27-30 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by Bis-Tris PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (263 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. G Reya T, et al. Thymic stromal-cell abnormalities and dysregulated T-cell development in IL-2-deficient mice. Dev Immunol. 1998;5(4):287-302. [Content Brief]
[2]. Zhu J, et al. Stat5 activation plays a critical role in Th2 differentiation. Immunity. 2003 Nov;19(5):739-48. [Content Brief]
[3]. Smith GA, et al. Essential biphasic role for JAK3 catalytic activity in IL-2 receptor signaling. Nat Chem Biol. 2016 May;12(5):373-9. [Content Brief]
[4]. Sim GC, et al. The IL-2 cytokine family in cancer immunotherapy. Cytokine Growth Factor Rev. 2014 Aug;25(4):377-90. [Content Brief]
[5]. Kim HP, et al. Both integrated and differential regulation of components of the IL-2/IL-2 receptor system. Cytokine Growth Factor Rev. 2006 Oct;17(5):349-66. [Content Brief]
[6]. Nelson BH. IL-2, regulatory T cells, and tolerance. J Immunol. 2004 Apr 1;172(7):3983-8. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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