IL-2R beta/CD122 Protein, Human (HEK293, His, SPR verified)
Based on 1 Customer Validation
IL-2R beta (CD122), a type Ⅰ cytokine receptor expressed on T lymphocytes, is a receptor for IL-2 (Kd: 1 nM approximately). IL-2R beta mediates T cell immune responses, such as stimulating T cell proliferation and activating lymphokine-activated killer cells. IL-2R beta/CD122 Protein, Human (HEK293, His) is a recombinant human extracellular region of IL-2R beta (A27-D239) with a C-Terminal 10*His tags, which is produced in HEK293 cells.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
IL-2R beta (CD122), a type Ⅰ cytokine receptor expressed on T lymphocytes, is a receptor for IL-2 (Kd: 1 nM approximately)[1]. IL-2R beta mediates T cell immune responses, such as stimulating T cell proliferation and activating lymphokine-activated killer cells[2]. IL-2R beta/CD122 Protein, Human (HEK293, His) is a recombinant human extracellular region of IL-2R beta (A27-D239) with a C-Terminal 10*His tags, which is produced in HEK293 cells.
Background
IL-2R beta (CD122) is a type I cytokine receptor, and belongs to Type 4 subfamily. IL-2R beta is also a key component of the IL-15 receptor. IL-2R beta is broadly expressed in spleen, blood, and lymph node, such as B and T lymphocytes[1][3].
The sequence of amino acids in IL-2R beta differs in different species. Human IL-2R beta shares <60% aa sequence identity with mouse and rats.
IL-2R beta cytoplasmic domain heterodimerizes with IL-2 and leads to the activation of signaling pathways: phosphoinositol 3-kinase (PI3-K)/AKT, Ras-MAP kinase, and the JAK-STAT pathways[4]. IL-2R beta binds IL-2 with intermediate affinity. IL-2R beta mediates IL-2 internalization and signal transduction, such as cell proliferation or differentiation[5]. IL-2R beta interacts with IL-2 and increases the proportion of CD4+ T lymphocytes[1]. IL-2R stimulates T cell proliferation and activating lymphokine-activated killer cells[2].
IL-2R beta mediates T cell immune responses, and also mediates endocytosis, as well as transducing the mitogenic signals of IL-2.
Verified Bioactivity
1.Human IL-2 R beta inhibits the IL-15 (HY-P7034) dependent proliferation of Mo7e cells. The ED50 for this effect is 0.391 µg/mL. corresponding to a specific activity is 2558.199 U/mg.
2.Human IL-2 R beta Protein immobilized on CM5 Chip via anti-His antibody can bind Human IL-2 Protein (HY-P7037) with an affinity constant of 0.116 μM as determined in a SPR assay.
3.Human IL-2 R beta Protein immobilized on CM5 via anti-His antibody can bind Human IL-15 Protein (HY-P7034) with an affinity constant of 5.51 nM as determined in a SPR assay.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-10*His
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Accession
P14784/NP_000869.1 (A27-D239)
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Gene ID3560
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Molecular Construction
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N-term
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IL-2Rβ (A27-D239)
Accession # P14784/NP_000869.1 -
10*His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
IL2RB; Interleukin 2 Receptor, Beta; Prev. IL15RB; IL-2R Subunit Beta; High Affinity IL-2 Receptor Subunit Beta; CD122 Antigen; Interleukin-2 Receptor Subunit Beta; IL-2RB; P70-75; P75; CD122; High Affinity IL-2 Receptor Beta Subunit; Interleukin-15 Receptor Subunit Beta; IMD63; Interleukin 15 Receptor, Beta; Interleukin 2 Receptor Subunit Beta; IL-2 Receptor Subunit Beta
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AA Sequence
AVNGTSQFTCFYNSRANISCVWSQDGALQDTSCQVHAWPDRRRWNQTCELLPVSQASWACNLILGAPDSQKLTTVDIVTLRVLCREGVRWRVMAIQDFKPFENLRLMAPISLQVVHVETHRCNISWEISQASHYFERHLEFEARTLSPGHTWEEAPLLTLKQKQEWICLETLTPDTQYEFQVRVKPLQGEFTTWSPWSQPLAFRTKPAALGKD
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Predicted Molecular Mass
26 kDa
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Molecular Weight
Approximately 35-48 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.; ≥ 95%, as determined by HPLC.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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