IL-4R alpha protein, Canine (HEK293, His)

IL-4R alpha is the α subunit shared by the IL-4 and IL-13 receptors and was initially discovered in leukocytes. IL-4R alpha is coupled to the JAK1/2/3-STAT6 pathway and participates in promoting Th2 differentiation. IL-4R alpha binds to IL-4, causing phosphorylation of its C-terminal tyrosine residue. IL-4R alpha possesses an immunoreceptor tyrosine inhibitor motif (ITIM), and by phosphorylating ITIM, it can bind to the SH2 domain of a partial phosphatase. IL-4R alpha contains a transmembrane domain. IL-4R alpha protein, Canine is expressed by HEK293 cells and includes a type III fibronectin domain and a C-terminal C-6*His tag^[1][2][3][4].

Measured by its ability to inhibit IL-4-dependent proliferation of TF 1 human erythroleukemic cells. The ED₅₀ for this effect is ≤18.24 ng/mL in the presence 0.2 ng/mL of Recombinant Human IL-4, corresponding to a specific activity is ≥5.482×10⁴ units/mg.

• 
  Measured by its ability to inhibit IL-4-dependent proliferation of TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 18.24 ng/mL in the presence 0.2 ng/mL of Recombinant Human IL‑4, corresponding to a specific activity is 5.482×10⁴ units/mg.

Canine

HEK293

C-6*His

XP_022275636.1 (G2-Q230)

489957  [NCBI]

Partial

GRLCSGLTFPVSCLVLVWVASSGSVKVLHEPSCFSDYISTSVCQWKMDHPTNCSAELRLSYQLDFMGSENHTCVPENREDSVCVCSMPIDDAVEADVYQLDLWAGQQLLWSGSFQPSKHVKPRTPGNLTVHPNISHTWLLMWTNPYPTENHLHSELTYMVNVSNDNDPEDFKVYNVTYMGPTLRLAASTLKSGASYSARVRAWAQTYNSTWSDWSPSTTWLNYYEPWEQ

Approximately 40-60 kDa

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Keegan AD,et al. An IL-4 receptor region containing an insulin receptor motif is important for IL-4-mediated IRS-1 phosphorylation and cell growth. Cell. 1994 Mar 11;76(5):811-20.  [Content Brief]

  [2]. Zurawski SM, et al. The primary binding subunit of the human interleukin-4 receptor is also a component of the interleukin-13 receptor. J Biol Chem. 1995 Jun 9;270(23):13869-78.  [Content Brief]

  [3]. Rolling C, et al. IL4 and IL13 receptors share the gamma c chain and activate STAT6, STAT3 and STAT5 proteins in normal human B cells. FEBS Lett. 1996 Sep 9;393(1):53-6.  [Content Brief]

  [4]. Jung T, et al. Soluble human interleukin-4 receptor is produced by activated T cells under the control of metalloproteinases. Int Arch Allergy Immunol. 1999 May;119(1):23-30.  [Content Brief]