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IL-4R alpha protein, Canine (HEK293, His)

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Based on 1 Customer Validation

IL-4R alpha is the α subunit shared by the IL-4 and IL-13 receptors and was initially discovered in leukocytes. IL-4R alpha is coupled to the JAK1/2/3-STAT6 pathway and participates in promoting Th2 differentiation. IL-4R alpha binds to IL-4, causing phosphorylation of its C-terminal tyrosine residue. IL-4R alpha possesses an immunoreceptor tyrosine inhibitor motif (ITIM), and by phosphorylating ITIM, it can bind to the SH2 domain of a partial phosphatase. IL-4R alpha contains a transmembrane domain. IL-4R alpha protein, Canine is expressed by HEK293 cells and includes a type III fibronectin domain and a C-terminal C-6*His tag.

For research use only. We do not sell to patients.
  • Species: Canine
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for IL-4R alpha protein, Canine (HEK293, His)
  • Experimental Validation Results for IL-4R alpha protein, Canine (HEK293, His)
  • IL-4R alpha protein, Canine (HEK293, His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
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Biological Activity

Description

IL-4R alpha is the α subunit shared by the IL-4 and IL-13 receptors and was initially discovered in leukocytes. IL-4R alpha is coupled to the JAK1/2/3-STAT6 pathway and participates in promoting Th2 differentiation. IL-4R alpha binds to IL-4, causing phosphorylation of its C-terminal tyrosine residue. IL-4R alpha possesses an immunoreceptor tyrosine inhibitor motif (ITIM), and by phosphorylating ITIM, it can bind to the SH2 domain of a partial phosphatase. IL-4R alpha contains a transmembrane domain. IL-4R alpha protein, Canine is expressed by HEK293 cells and includes a type III fibronectin domain and a C-terminal C-6*His tag[1][2][3][4].

Verified Bioactivity

Measured by its ability to inhibit IL-4-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is ≤18.24 ng/mL in the presence 0.2 ng/mL of Recombinant Human IL-4, corresponding to a specific activity is ≥5.482×104 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to inhibit IL-4-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 18.24 ng/mL in the presence 0.2 ng/mL of Recombinant Human IL‑4, corresponding to a specific activity is 5.482×104 units/mg.

Technical Parameters

  • Species Canine
  • Source HEK293
  • Tag C-6*His
  • Accession

    XP_022275636.1 (V26-Q230)

  • Gene ID
    489957  [NCBI]
  • Molecular Construction
    • N-term
    • IL-4Rα (V26-Q230)
      Accession # XP_022275636.1
    • 6*His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    IL4R; IL4RA; Interleukin 4 Receptor; Interleukin-4 Receptor Alpha Chain; Interleukin-4 Receptor Subunit Alpha; IL-4R Subunit Alpha; CD124; IL4R Nirs Variant 1; IL-4 Receptor Subunit Alpha; CD124 Antigen; Interleukin 13 Receptor; IL-4R-Alpha; IL-4RA

  • AA Sequence

    VKVLHEPSCFSDYISTSVCQWKMDHPTNCSAELRLSYQLDFMGSENHTCVPENREDSVCVCSMPIDDAVEADVYQLDLWAGQQLLWSGSFQPSKHVKPRTPGNLTVHPNISHTWLLMWTNPYPTENHLHSELTYMVNVSNDNDPEDFKVYNVTYMGPTLRLAASTLKSGASYSARVRAWAQTYNSTWSDWSPSTTWLNYYEPWEQ

  • Predicted Molecular Mass

    26 kDa

  • Molecular Weight

    Approximately 45 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Keegan AD,et al. An IL-4 receptor region containing an insulin receptor motif is important for IL-4-mediated IRS-1 phosphorylation and cell growth. Cell. 1994 Mar 11;76(5):811-20. [Content Brief]

[2]. Zurawski SM, et al. The primary binding subunit of the human interleukin-4 receptor is also a component of the interleukin-13 receptor. J Biol Chem. 1995 Jun 9;270(23):13869-78. [Content Brief]

[3]. Rolling C, et al. IL4 and IL13 receptors share the gamma c chain and activate STAT6, STAT3 and STAT5 proteins in normal human B cells. FEBS Lett. 1996 Sep 9;393(1):53-6. [Content Brief]

[4]. Jung T, et al. Soluble human interleukin-4 receptor is produced by activated T cells under the control of metalloproteinases. Int Arch Allergy Immunol. 1999 May;119(1):23-30. [Content Brief]

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Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to inhibit IL-4-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 18.24 ng/mL in the presence 0.2 ng/mL of Recombinant Human IL‑4, corresponding to a specific activity is 5.482×104 units/mg.

  • IL-4R alpha protein, Canine (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for IL-4R alpha protein, Canine (HEK293, His)
  • Experimental Validation Results for IL-4R alpha protein, Canine (HEK293, His)
  • IL-4R alpha protein, Canine (HEK293, His) Product Image