IL-5 Protein, Cynomolgus (HEK293, His)
IL-5 Protein, produced by T-lymphocytes and NK cells, is crucial for eosinophil survival, differentiation, and chemotaxis. It stimulates immunoglobulin production and growth in B-cells. IL-5 Protein activates kinases (LYN, SYK, and JAK2) through a receptor (IL5RA and CSF2RB subunits), propagating signals via RAS-MAPK and JAK-STAT5 pathways. It exists as a disulfide-linked homodimer. IL-5 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived IL-5 protein, expressed by HEK293, with N-His labeled tag.
- Species: Cynomolgus
- Source: HEK293
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
IL-5 Protein, produced by T-lymphocytes and NK cells, is crucial for eosinophil survival, differentiation, and chemotaxis. It stimulates immunoglobulin production and growth in B-cells. IL-5 Protein activates kinases (LYN, SYK, and JAK2) through a receptor (IL5RA and CSF2RB subunits), propagating signals via RAS-MAPK and JAK-STAT5 pathways. It exists as a disulfide-linked homodimer. IL-5 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived IL-5 protein, expressed by HEK293, with N-His labeled tag.
Background
IL-5 Protein is a homodimeric cytokine primarily produced by T-lymphocytes and NK cells, playing a crucial role in eosinophil survival, differentiation, and chemotaxis. It also acts on B-cells, stimulating immunoglobulin production, growth, and differentiation. IL-5 Protein exerts its effects through a receptor composed of IL5RA and CSF2RB subunits, leading to the activation of various kinases such as LYN, SYK, and JAK2. This activation propagates signals through the RAS-MAPK and JAK-STAT5 pathways. IL-5 Protein exists as a disulfide-linked homodimer.
Technical Parameters
-
Species Cynomolgus
-
Source HEK293
-
Tag N-His
-
Accession
A0A2K5U1E7 (I20-S134)
-
Gene ID/
-
Molecular Construction
-
N-term
-
His
-
IL-5 (I20-S134)
Accession # A0A2K5U1E7 -
C-term
-
-
Protein Length
Full Length of Mature Protein
-
Synonyms
IL5; T-Cell Replacing Factor; Interleukin 5; EDF; Eosinophil Differentiation Factor; Colony-Stimulating Factor, Eosinophil; Interleukin-5; B-Cell Differentiation Factor I; IL-5; Interleukin 5 (Colony-Stimulating Factor, Eosinophil); TRF; B Cell Differentiation Factor I
-
AA Sequence
IPTEIPTSALVKETLALLSTHRTLLIGNETLRIPVPVHKHHQLCTEEIFQGIGTLESQTVQGGTVERLFKNLSLIKKYIDGQKKKCGEERRRVNQFLDYLQEFLGVMNTEWIIES
-
Predicted Molecular Mass
15 kDa
-
Molecular Weight
Approximately 19-23 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
-
Glycosylation
Yes
-
Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
