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  4. Insulysin/IDE Protein, Human (HEK293, His)

Insulysin/IDE Protein, Human (HEK293, His)

Cat. No.: HY-P70291
COA Handling Instructions

Insulin/IDE protein is the key to the decomposition of cellular peptides. It participates in the degradation of insulin, APP peptides, IAPP peptides, natriuretic peptides, glucagon, bradykinin, kinins and other peptides, and affects intercellular peptide signaling. Substrate binding induces conformational changes that promote degradation of larger substrates such as insulin. Insulysin/IDE Protein, Human (HEK293, His) is the recombinant human-derived Insulysin/IDE protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Insulysin/IDE Protein, Human (HEK293, His) is 978 a.a., with molecular weight of ~120.0 kDa.

For research use only. We do not sell to patients.

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Description

Insulin/IDE protein is the key to the decomposition of cellular peptides. It participates in the degradation of insulin, APP peptides, IAPP peptides, natriuretic peptides, glucagon, bradykinin, kinins and other peptides, and affects intercellular peptide signaling. Substrate binding induces conformational changes that promote degradation of larger substrates such as insulin. Insulysin/IDE Protein, Human (HEK293, His) is the recombinant human-derived Insulysin/IDE protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Insulysin/IDE Protein, Human (HEK293, His) is 978 a.a., with molecular weight of ~120.0 kDa.

Background

Insulysin/IDE protein plays a crucial role in the intricate cellular breakdown of various peptides, including insulin, APP peptides, IAPP peptides, natriuretic peptides, glucagon, bradykinin, kallidin, and others. This multifaceted involvement underscores its significance in intercellular peptide signaling and the regulation of peptide hormone cascades. Substrate binding induces vital conformational changes, enabling the degradation of larger substrates, such as insulin. Insulysin/IDE contributes to the intricate regulation of blood glucose homeostasis by participating in the degradation of insulin, glucagon, and IAPP. Additionally, it plays a pivotal role in the clearance of amyloidogenic peptides derived from APP, impacting neuronal and microglial processes. The protein is also implicated in the degradation of natriuretic peptides, ANP, BNP, and CNP, thereby inactivating their ability to raise intracellular cGMP. Furthermore, Insulysin/IDE is involved in antigen processing, producing antigenic peptides presented to cytotoxic T lymphocytes. In microbial infection, the membrane-associated isoform acts as an entry receptor for varicella-zoster virus (VZV).

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Human

Source

HEK293

Tag

C-6*His

Accession

P14735 (M42-L1019)

Gene ID
Molecular Construction
N-term
Insulysin (M42-L1019)
Accession # P14735
6*His
C-term
Synonyms
rHuInsulin-degrading enzyme/IDE, His ; Insulin-Degrading Enzyme; Abeta-Degrading Protease; Insulin Protease; Insulinase; Insulysin; IDE
AA Sequence

MNNPAIKRIGNHITKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIAGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVRQELLKFHSAYYSSNLMAVCVLGRESLDDLTNLVVKLFSEVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNGKFKLPTKNEFIPTNFEILPLEKEATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNTEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPHINFMAAKL

Molecular Weight

Approximately 120.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 10% Glycerol, pH 7.5.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

Insulysin/IDE Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Insulysin/IDE Protein, Human (HEK293, His)
Cat. No.:
HY-P70291
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