Insulysin/IDE Protein, Human (HEK293, His)
Based on 1 Customer Validation
Insulin/IDE protein is the key to the decomposition of cellular peptides. It participates in the degradation of insulin, APP peptides, IAPP peptides, natriuretic peptides, glucagon, bradykinin, kinins and other peptides, and affects intercellular peptide signaling. Substrate binding induces conformational changes that promote degradation of larger substrates such as insulin. Insulysin/IDE Protein, Human (HEK293, His) is the recombinant human-derived Insulysin/IDE protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Insulin/IDE protein is the key to the decomposition of cellular peptides. It participates in the degradation of insulin, APP peptides, IAPP peptides, natriuretic peptides, glucagon, bradykinin, kinins and other peptides, and affects intercellular peptide signaling. Substrate binding induces conformational changes that promote degradation of larger substrates such as insulin. Insulysin/IDE Protein, Human (HEK293, His) is the recombinant human-derived Insulysin/IDE protein, expressed by HEK293 , with C-6*His labeled tag.
Background
Insulysin/IDE protein plays a crucial role in the intricate cellular breakdown of various peptides, including insulin, APP peptides, IAPP peptides, natriuretic peptides, glucagon, bradykinin, kallidin, and others. This multifaceted involvement underscores its significance in intercellular peptide signaling and the regulation of peptide hormone cascades. Substrate binding induces vital conformational changes, enabling the degradation of larger substrates, such as insulin. Insulysin/IDE contributes to the intricate regulation of blood glucose homeostasis by participating in the degradation of insulin, glucagon, and IAPP. Additionally, it plays a pivotal role in the clearance of amyloidogenic peptides derived from APP, impacting neuronal and microglial processes. The protein is also implicated in the degradation of natriuretic peptides, ANP, BNP, and CNP, thereby inactivating their ability to raise intracellular cGMP. Furthermore, Insulysin/IDE is involved in antigen processing, producing antigenic peptides presented to cytotoxic T lymphocytes. In microbial infection, the membrane-associated isoform acts as an entry receptor for varicella-zoster virus (VZV).
Verified Bioactivity
The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
P14735-1 (M42-L1019)
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Molecular Construction
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N-term
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Insulysin (M42-L1019)
Accession # P14735-1 -
6*His
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C-term
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Protein Length
Partial
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Synonyms
IDE; Insulin Protease; Insulin Degrading Enzyme; Abeta-Degrading Protease; Insulin-Degrading Enzyme; Insulinase; Insulysin; CDNA FLJ35968 Fis, Clone TESTI2013053, Highly Similar To INSULIN-DEGRADING ENZYME
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AA Sequence
MNNPAIKRIGNHITKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIAGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVRQELLKFHSAYYSSNLMAVCVLGRESLDDLTNLVVKLFSEVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNGKFKLPTKNEFIPTNFEILPLEKEATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNTEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPHINFMAAKL
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Predicted Molecular Mass
114.3 kDa
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Molecular Weight
Approximately 100-130 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 10% Glycerol, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (241 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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