ISG15 E1/UBE1L Protein, Human (sf9, His, StrepⅡ)
Based on 1 Customer Validation
The ISG15 E1/UBE1L protein initiates ubiquitination by adenylating the C-terminal glycine of ubiquitin with ATP to form a thioester bond with its cysteine residue. This produces ubiquitin-E1 thioester and free AMP. ISG15 E1/UBE1L Protein, Human (sf9, His, Strep) is the recombinant human-derived ISG15 E1/UBE1L protein, expressed by Sf9 insect cells , with N-8*His, N-Strep labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The ISG15 E1/UBE1L protein initiates ubiquitination by adenylating the C-terminal glycine of ubiquitin with ATP to form a thioester bond with its cysteine residue. This produces ubiquitin-E1 thioester and free AMP. ISG15 E1/UBE1L Protein, Human (sf9, His, Strep) is the recombinant human-derived ISG15 E1/UBE1L protein, expressed by Sf9 insect cells , with N-8*His, N-Strep labeled tag.
Background
ISG15 E1/UBE1L protein initiates the ubiquitination process by activating ubiquitin through a two-step mechanism. It begins by adenylating the C-terminal glycine residue of ubiquitin with ATP and subsequently forms a thioester bond between this glycine residue and a cysteine residue in E1. This results in the generation of a ubiquitin-E1 thioester and free AMP. In addition to its role in ubiquitin activation, ISG15 E1/UBE1L catalyzes the ISGylation of the influenza A virus NS1 protein, highlighting its involvement in antiviral responses. The protein is actively engaged in protein modification processes, particularly protein ubiquitination, underscoring its significance in regulating cellular pathways through post-translational modifications.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-StrepⅡ;N-8*His
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Accession
P41226 (D2-L1012)
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Molecular Construction
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N-term
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8*His-StrepⅡ
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UBE1L (D2-L1012)
Accession # P41226 -
C-term
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Protein Length
Full Length of Ubiquitin-like modifier-activating enzyme 7 Chain
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Synonyms
UBA7; Ubiquitin Like Modifier Activating Enzyme 7; UBE1L; UBE2; D8; UBA1B; Ubiquitin-Like Modifier-Activating Enzyme 7; Ubiquitin-Activating Enzyme E1 Homolog; UBA7, Ubiquitin-Activating Enzyme E1; Ubiquitin-Activating Enzyme 7; UBA1, Ubiquitin-Activating
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AA Sequence
DALDASKLLDEELYSRQLYVLGSPAMQRIQGARVLVSGLQGLGAEVAKNLVLMGVGSLTLHDPHPTCWSDLAAQFLLSEQDLERSRAEASQELLAQLNRAVQVVVHTGDITEDLLLDFQVVVLTAAKLEEQLKVGTLCHKHGVCFLAADTRGLVGQLFCDFGEDFTVQDPTEAEPLTAAIQHISQGSPGILTLRKGANTHYFRDGDLVTFSGIEGMVELNDCDPRSIHVREDGSLEIGDTTTFSRYLRGGAITEVKRPKTVRHKSLDTALLQPHVVAQSSQEVHHAHCLHQAFCALHKFQHLHGRPPQPWDPVDAETVVGLARDLEPLKRTEEEPLEEPLDEALVRTVALSSAGVLSPMVAMLGAVAAQEVLKAISRKFMPLDQWLYFDALDCLPEDGELLPSPEDCALRGSRYDGQIAVFGAGFQEKLRRQHYLLVGAGAIGCELLKVFALVGLGAGNSGGLTVVDMDHIERSNLSRQFLFRSQDVGRPKAEVAAAAARGLNPDLQVIPLTYPLDPTTEHIYGDNFFSRVDGVAAALDSFQARRYVAARCTHYLKPLLEAGTSGTWGSATVFMPHVTEAYRAPASAAASEDAPYPVCTVRYFPSTAEHTLQWARHEFEELFRLSAETINHHQQAHTSLADMDEPQTLTLLKPVLGVLRVRPQNWQDCVAWALGHWKLCFHYGIKQLLRHFPPNKVLEDGTPFWSGPKQCPQPLEFDTNQDTHLLYVLAAANLYAQMHGLPGSQDWTALRELLKLLPQPDPQQMAPIFASNLELASASAEFGPEQQKELNKALEVWSVGPPLKPLMFEKDDDSNFHVDFVVAAASLRCQNYGIPPVNRAQSKRIVGQIIPAIATTTAAVAGLLGLELYKVVSGPRPRSAFRHSYLHLAENYLIRYMPFAPAIQTFHHLKWTSWDRLKVPAGQPERTLESLLAHLQEQHGLRVRILLHGSALLYAAGWSPEKQAQHLPLRVTELVQQLTGQAPAPGQRVLVLELSCEGDDEDTAFPPLHYEL
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Predicted Molecular Mass
114.7 KDa
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH7.5, 200 mM NaCl, 1 mM DTT, 20% glycerol.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (242 KB)
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SDS (311 KB)
- English - EN (311 KB)
- Français - FR (311 KB)
- Deutsch - DE (311 KB)
- Norwegian - NO (311 KB)
- Español - ES (311 KB)
- Swedish - SV (311 KB)
- Italian - IT (311 KB)
- Korean - KR (311 KB)
- Portuguese - PT (311 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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