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Jagged-1/JAG1 Protein, Human (HEK293, His)

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Jagged-1/JAG1 protein is a ligand for multiple Notch receptors and crucially mediates Notch signaling through interactions with NOTCH1, NOTCH2, and NOTCH3. Its role in hematopoiesis suggests its involvement in the regulation of blood cell development. Jagged-1/JAG1 Protein, Human (HEK293, His) is the recombinant human-derived Jagged-1/JAG1 protein, expressed by HEK293 , with C-10*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Jagged-1/JAG1 Protein, Human (HEK293, His)
  • Experimental Validation Results for Jagged-1/JAG1 Protein, Human (HEK293, His)
  • Jagged-1/JAG1 Protein, Human (HEK293, His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

Jagged-1/JAG1 protein is a ligand for multiple Notch receptors and crucially mediates Notch signaling through interactions with NOTCH1, NOTCH2, and NOTCH3. Its role in hematopoiesis suggests its involvement in the regulation of blood cell development. Jagged-1/JAG1 Protein, Human (HEK293, His) is the recombinant human-derived Jagged-1/JAG1 protein, expressed by HEK293 , with C-10*His labeled tag.

Background

The Jagged-1 (JAG1) protein functions as a ligand for multiple Notch receptors, playing a crucial role in mediating Notch signaling, as evidenced by its interactions with NOTCH1, NOTCH2, and NOTCH3. Its involvement in cell-fate decisions during hematopoiesis suggests a regulatory role in blood cell development. JAG1 is implicated in various stages of mammalian cardiovascular development and has been associated with both early and late phases of this process. Additionally, it inhibits myoblast differentiation and enhances fibroblast growth factor-induced angiogenesis in vitro. Notably, the interaction between JAG1 and NOTCH1 is calcium ion-dependent, highlighting the complexity of its regulatory mechanisms in cellular processes.

Verified Bioactivity

Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells and the ED50 is ≤20 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.3007 μg/mL in the presence of 500 ng/mL BMP-2, corresponding to a specific activity is 3.32×103 U/mg

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-10*His
  • Accession

    P78504-1/NP_000205.1 (Q34-S1046)

  • Gene ID
    182  [NCBI]
  • Molecular Construction
    • N-term
    • JAG1 (Q34-S146)
      Accession # P7854-1/NP_25.1
    • 1*His
    • C-term
  • Protein Length

    Partial Extracellular Domain

  • Synonyms

    JAG1; Jagged 1; Prev. JAGL1; Alagille Syndrome; Prev. AGS; Jagged-1 Protein; HJ1; CD339 Antigen; CD339; Jagged1; AHD; CMT2HH; AWS; AGS1; Protein Jagged-1; DCHE; Jagged Canonical Notch Ligand 1; Delta-Like Protein

  • AA Sequence

    QFELEILSMQNVNGELQNGNCCGGARNPGDRKCTRDECDTYFKVCLKEYQSRVTAGGPCSFGSGSTPVIGGNTFNLKASRGNDRNRIVLPFSFAWPRSYTLLVEAWDSSNDTVQPDSIIEKASHSGMINPSRQWQTLKQNTGVAHFEYQIRVTCDDYYYGFGCNKFCRPRDDFFGHYACDQNGNKTCMEGWMGPECNRAICRQGCSPKHGSCKLPGDCRCQYGWQGLYCDKCIPHPGCVHGICNEPWQCLCETNWGGQLCDKDLNYCGTHQPCLNGGTCSNTGPDKYQCSCPEGYSGPNCEIAEHACLSDPCHNRGSCKETSLGFECECSPGWTGPTCSTNIDDCSPNNCSHGGTCQDLVNGFKCVCPPQWTGKTCQLDANECEAKPCVNAKSCKNLIASYYCDCLPGWMGQNCDININDCLGQCQNDASCRDLVNGYRCICPPGYAGDHCERDIDECASNPCLNGGHCQNEINRFQCLCPTGFSGNLCQLDIDYCEPNPCQNGAQCYNRASDYFCKCPEDYEGKNCSHLKDHCRTTPCEVIDSCTVAMASNDTPEGVRYISSNVCGPHGKCKSQSGGKFTCDCNKGFTGTYCHENINDCESNPCRNGGTCIDGVNSYKCICSDGWEGAYCETNINDCSQNPCHNGGTCRDLVNDFYCDCKNGWKGKTCHSRDSQCDEATCNNGGTCYDEGDAFKCMCPGGWEGTTCNIARNSSCLPNPCHNGGTCVVNGESFTCVCKEGWEGPICAQNTNDCSPHPCYNSGTCVDGDNWYRCECAPGFAGPDCRININECQSSPCAFGATCVDEINGYRCVCPPGHSGAKCQEVSGRPCITMGSVIPDGAKWDDDCNTCQCLNGRIACSKVWCGPRPCLLHKGHSECPSGQSCIPILDDQCFVHPCTGVGECRSSSLQPVKTKCTSDSYYQDNCANITFTFNKEMMSPGLTTEHICSELRNLNILKNVSAEYSIYIACEPSPSANNEIHVAISAEDIRDDGNPIKEITDKIIDLVSKRDGNS

  • Molecular Weight

    Approximately 130-160 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol, 0.01% Tween 80.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
3.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.3007 μg/mL in the presence of 500 ng/mL BMP-2, corresponding to a specific activity is 3.32×103 U/mg

  • Jagged-1/JAG1 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Jagged-1/JAG1 Protein, Human (HEK293, His)
  • Experimental Validation Results for Jagged-1/JAG1 Protein, Human (HEK293, His)
  • Jagged-1/JAG1 Protein, Human (HEK293, His) Product Image